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Träfflista för sökning "WFRF:(Larsson Anders 1957 ) ;srt2:(1995-1999)"

Sökning: WFRF:(Larsson Anders 1957 ) > (1995-1999)

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1.
  • Odin, Elisabeth, 1955, et al. (författare)
  • Rapid method for relative gene expression determination in human tissues using automated capillary gel electrophoresis and multicolor detection
  • 1999
  • Ingår i: J Chromatogr B Biomed Sci Appl. - 1387-2273. ; 734:1, s. 47-53
  • Tidskriftsartikel (refereegranskat)abstract
    • The aim of this study was to evaluate a direct and automated post-polymerase chain reaction (PCR) detection system to simultaneously determine the relative gene expression levels of nine cancer-related human genes. Total RNA was prepared from flash-frozen biopsies derived from human colorectal tumors or normal mucosa and reverse-transcribed to cDNA which was PCR-amplified using primer pairs corresponding to the studied genes. In each reaction, the forward primer was labeled with a fluorescent dye. The PCR products were pooled and an internal size standard with a uniquely colored fluorescent dye was added. The samples were then subjected to automated capillary gel electrophoresis. Fragment analysis software was used to calculate the relative gene expression using beta-actin as the reference gene. We found that automated capillary gel electrophoresis with multicolor detection is a rapid, accurate and highly reproducible method for separation and quantification of PCR-amplified cDNA.
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