| 1. |
|
|
| 2. |
- Huang, Ranyang, et al.
(författare)
-
Expression of a Mast Cell Tryptase in the Human Monocytic Cell Lines U-937 and Mono Mac 6
- 1993
-
Ingår i: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 38:4, s. 359-367
-
Tidskriftsartikel (refereegranskat)abstract
- Expression of a mast cell tryptase mRNA was detected in two human monocytic cell lines, the U-937 and the Mono Mac 6, and in normal human peripheral blood(PB) monocytes. In the U-937 cell line but not in normal PB monocytes, the tryptase expression was upregulated 3-50 fold following phorbol ester (PMA)-induced differentiation, but no such induction was seen after retinoic acid, interferon-gamma or vitamin D3 exposure. The tryptases expressed in PMA-induced and non-induced U-937 and in Mono Mac 6 were characterized by PCR amplification and nucleotide sequence analysis. The U-937 cell line was found to express a tryptase identical to one of the previously cloned mast-cell beta tryptases (Tryptase I), and the tryptase expressed in Mono Mac 6 was found to be nearly identical to the previously cloned alpha tryptase. By northern blot analysis with oligonucleotide probes specific for the alpha and beta tryptases both cell lines were found to express only one type of tryptase. Densitometric quantifications of tryptase mRNA levels, in the two cell lines, showed approximately 80 times higher mRNA levels in Mono Mac 6 compared to non-induced U-937. Immunohistochemical staining for tryptase showed a marked heterogeneity in the Mono Mac 6 cell line. Only one out of 10 cells were positive for the protein but the levels in these cells were very high, equivalent, or even higher than the levels seen in the human mast cell line HMC-1. This shows that the expression of a single tryptase, in this case the alpha tryptase, is sufficient for the production of a stable protein and probably also a stable proteolytically active tetramer. The family of human mast-cell tryptases has been considered to represent a class of proteases specifically expressed in mast cells and basophilic leucocytes. The expression of tryptases in two monocytic cell lines and in normal PB monocytes indicate that in humans, the lineage specificity of these serine proteases is less restricted than earlier expected. The cloning of a full length cDNA for the murine counterpart to the human mast cell tryptases, the MMCP-6, is presented. No expression of the MMCP-6 was detected in a panel of mouse monocyte or macrophage cell lines indicating a species difference in the lineage specificity of the 'mast cell tryptases'.
|
|
| 3. |
- Jernelöv, Arne, et al.
(författare)
-
Tankar kring begreppet - Sustainable Development
- 1990
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Begreppet 'sustainable development' har fått omfattande politisk acceptans men är inte ett operationellt koncept eftersom det är odefinierat och opreciserat. I artikeln diskuteras frågan om tidshorisont, huruvida begreppet bör avse teknologier eller projekt och hur en analys av 'sustainability' kan gå till.
|
|
| 4. |
- Larsson, Mats, et al.
(författare)
-
Archaeological field survey - methods and problems
- 1992
-
Ingår i: The Archaeology of the Cultural Landscape. Field work and research in a south Swedish rural region. - 0065-1001. - 9122015507 ; 19
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The advantages and disadvantages of field survey as a method for locating possible Stone and Bronze Age sites are described. Survey in the spring was favorable for finding Stone Age sites, while autumn survey was more successful for locating Bronze Age sites.
|
|
| 5. |
- Lindberg, Lars, et al.
(författare)
-
Nitric oxide gives maximal response after coronary artery bypass surgery
- 1994
-
Ingår i: Journal of Cardiothoracic and Vascular Anesthesia. - : Elsevier BV. - 1532-8422 .- 1053-0770. ; 8:2, s. 182-187
-
Tidskriftsartikel (refereegranskat)abstract
- The dose-response to inhalation of nitric oxide (NO) after coronary artery bypass surgery was studied in seven patients with normal preoperative lung function and chest radiograms. During postoperative controlled ventilation with PEEP 5 and 10 cmH2O, the patients inhaled NO in concentrations of 2 to 25 ppm, in random order, for 6 to 10 minutes. Hemodynamic and oximetric data were analyzed before, 5 minutes after start of the NO inhalation, and 5 minutes after the cessation. The response was the same at all concentrations; mean pulmonary artery pressure decreased by 11 +/- 1% (P < 0.05) and pulmonary vascular resistance decreased by 22 +/- 2% (P < 0.05). Systemic hemodynamics did not change, but oximetric parameters tended to improve. Changes in PEEP did not affect the response. It is concluded that, in patients who have undergone coronary artery bypass grafting, inhalation of 2 to 25 ppm NO causes a dose-independent decrease in pulmonary artery pressure and pulmonary vascular resistance. In order to investigate the dose-response curve, concentrations lower than 2 ppm of NO must be used.
|
|
| 6. |
- Odin, Elisabeth, 1955, et al.
(författare)
-
Rapid method for relative gene expression determination in human tissues using automated capillary gel electrophoresis and multicolor detection
- 1999
-
Ingår i: J Chromatogr B Biomed Sci Appl. - 1387-2273. ; 734:1, s. 47-53
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to evaluate a direct and automated post-polymerase chain reaction (PCR) detection system to simultaneously determine the relative gene expression levels of nine cancer-related human genes. Total RNA was prepared from flash-frozen biopsies derived from human colorectal tumors or normal mucosa and reverse-transcribed to cDNA which was PCR-amplified using primer pairs corresponding to the studied genes. In each reaction, the forward primer was labeled with a fluorescent dye. The PCR products were pooled and an internal size standard with a uniquely colored fluorescent dye was added. The samples were then subjected to automated capillary gel electrophoresis. Fragment analysis software was used to calculate the relative gene expression using beta-actin as the reference gene. We found that automated capillary gel electrophoresis with multicolor detection is a rapid, accurate and highly reproducible method for separation and quantification of PCR-amplified cDNA.
|
|
| 7. |
|
|
| 8. |
- Sandström, Olof, et al.
(författare)
-
Miljöpåverkan av skogsindustriutsläpp
- 1997
-
Ingår i: Naturvårdsverkets rapportserie. Rapport 4695. ; , s. 1-89
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 9. |
|
|
| 10. |
- Adlerberth, Ingegerd, 1959, et al.
(författare)
-
High turnover rate of Escherichia coli strains in the intestinal flora of infants in Pakistan.
- 1998
-
Ingår i: Epidemiology and infection. - 0950-2688. ; 121:3, s. 587-98
-
Tidskriftsartikel (refereegranskat)abstract
- The Escherichia coli flora of infants in developed countries is dominated by one or a few strains which persist for prolonged periods of time, but no longitudinal studies have been performed in developing countries. To this end, we studied the rectal enterobacterial flora in 22 home-delivered Pakistani infants during their first 6 months of life. Three colonies were isolated and species typed on each of 11 sampling occasions. E. coli isolates were strain typed using electromorphic typing of cytoplasmic enzymes, and their O serogroups were determined. There was a very rapid turnover of enterobacterial strains in the rectal flora of individual infants. On average, 8.5 different E. coli strains were found per infant, and several biotypes of other enterobacteria. Less than 50% of the infants were colonized with E. coli from their mothers, but strains of maternal origin were four times more likely to persists in the infants' flora than other E. coli strains. Enterobacteria other than E. coli were always of non-maternal origin, and Enterobacter cloacae and Klebsiella pneumoniae biotypes recovered from contaminated feeds were later identified in the infants' rectal flora. An early colonization with klebsiella or enterobacter was significantly associated with diarrhoea during the neonatal period, although these bacteria were not likely to be the cause of the disease. The results suggest that poor hygienic conditions result in an unstable and diverse enterobacterial flora, which may influence infant health.
|
|
