| 1. |
- Bohgard, Mats, et al.
(författare)
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Characterizations of Radon Daughters in Indoor Air
- 1989
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Ingår i: Proceedings of Contribution to the symposium of the Nordic Society for Aerosol Research, Copenhagen, 1989.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Dierckx, Anke, 1986
(författare)
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Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions
- 2014
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Ever since unravelling the structure of DNA, an expanding research field has emerged with ongoing efforts dedicated to increase our understanding of the molecule of life. Since the natural nucleobases are virtually non-emissive, it has been a challenge for decades to ‘light up’ DNA/RNA in order to investigate their properties utilizing fluorescence techniques. This thesis focuses on fluorescent nucleobase analogues (FBAs) as probes for fluorescently labeling DNA and investigating its interactions, for example, with proteins. These artificial nucleobases attempt to closely mimic the characteristics of natural bases, while introducing fluorescence properties to the system. The first part of this work comprises the characterization of both new and established FBAs. Photophysical and base-mimicking properties of two fluorescent adenine analogues, triazole adenine (AT) and quadracyclic adenine (qA) are presented. Both exhibit promising features compared to the widely used commercially available adenine mimic 2-aminopurine (2-AP). Even though AT shows promising emission as a monomer and in certain DNA surroundings, it destabilizes the B-DNA duplex structure, most likely due to its C8-triazole extension. In order to overcome this effect, a new family of triazole adenine analogues extended on the 7-position was synthesized and photophysically characterized. The second thoroughly characterized adenine analogue, qA, is moderately fluorescent both as a monomer and inside DNA but in contrast to AT, the two-ring extension on qA is suggested to be well accommodated in the major groove and renders the DNA-duplex unperturbed or even stabilized, depending on the surrounding sequence. Finally, the photostability of tC, an already established FBA of the tricyclic cytosine family, was investigated. The latter yields a single photoproduct with a decreased fluorescence, which destabilizes DNA duplexes.In the second part of this work, the tricyclic cytosine FBA FRET-pair, tCO-tCnitro was applied in exploring the role of the mammalian transcription factor A in mitochondrial transcription. Furthermore, it was called upon to help resolve the order of events in which the different components of the transcription machinery initiate transcription.
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| 3. |
- Gudmundson, Peter
(författare)
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Foreword (President, KTH)
- 2014
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Ingår i: One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014. - : CRC Press/Balkema. ; , s. xxxiii-xxxiv
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Konferensbidrag (refereegranskat)
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| 4. |
- Gudmundson, Peter
(författare)
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Foreword (President, KTH)
- 2010
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Ingår i: Arsenic in Geosphere and Human Diseases, As 2010 - 3rd International Congress: Arsenic in the Environment. ; , s. xxv-xxvi
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Konferensbidrag (refereegranskat)
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| 5. |
- Gudmundson, Peter
(författare)
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Foreword (President, KTH)
- 2012
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Ingår i: Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. ; , s. xxi-xxii
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Konferensbidrag (refereegranskat)
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| 6. |
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| 7. |
- Wienberg, Jes
(författare)
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Sveriges kyrkor och Hallands sockenkyrkor
- 2003
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Ingår i: META: Medeltidsarkeologisk tidskrift. - 0348-7903. ; :1, s. 57-60
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Recension (övrigt vetenskapligt/konstnärligt)abstract
- Churches of Sweden and the Parish Churches of Halland: Comments on the “Churches of Sweden”, the Parish Church Project and the first report from the project, which presents the parish churches of Halland.
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| 8. |
- Wienberg, Jes
(författare)
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Vindmøller, men ingen mel
- 1999
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Ingår i: META: Medeltidsarkeologisk tidskrift. - 0348-7903. ; :3, s. 29-30
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Windmills, but no flour: A reply on Stefan Larssons comments on my article, "Flugten fra middelalderen" (The escape from the Middle Ages) in META 1999: 1
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| 9. |
- Wienecke, J, et al.
(författare)
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Global gene expression analysis of rodent motor neurons following spinal cord injury associates molecular mechanisms with development of postinjury spasticity
- 2010
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Ingår i: Journal of neurophysiology. - : American Physiological Society. - 1522-1598 .- 0022-3077. ; 103:2, s. 761-778
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Tidskriftsartikel (refereegranskat)abstract
- Spinal cord injury leads to severe problems involving impaired motor, sensory, and autonomic functions. After spinal injury there is an initial phase of hyporeflexia followed by hyperreflexia, often referred to as spasticity. Previous studies have suggested a relationship between the reappearance of endogenous plateau potentials in motor neurons and the development of spasticity after spinalization. To unravel the molecular mechanisms underlying the increased excitability of motor neurons and the return of plateau potentials below a spinal cord injury we investigated changes in gene expression in this cell population. We adopted a rat tail-spasticity model with a caudal spinal transection that causes a progressive development of spasticity from its onset after 2 to 3 wk until 2 mo postinjury. Gene expression changes of fluorescently identified tail motor neurons were studied 21 and 60 days postinjury. The motor neurons undergo substantial transcriptional regulation in response to injury. The patterns of differential expression show similarities at both time points, although there are 20% more differentially expressed genes 60 days compared with 21 days postinjury. The study identifies targets of regulation relating to both ion channels and receptors implicated in the endogenous expression of plateaux. The regulation of excitatory and inhibitory signal transduction indicates a shift in the balance toward increased excitability, where the glutamatergic N-methyl-d-aspartate receptor complex together with cholinergic system is up-regulated and the γ-aminobutyric acid type A receptor system is down-regulated. The genes of the pore-forming proteins Cav1.3 and Nav1.6 were not up-regulated, whereas genes of proteins such as nonpore-forming subunits and intracellular pathways known to modulate receptor and channel trafficking, kinetics, and conductivity showed marked regulation. On the basis of the identified changes in global gene expression in motor neurons, the present investigation opens up for new potential targets for treatment of motor dysfunction following spinal cord injury.
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