SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Förlin Lars 1950 ) ;lar1:(gu);pers:(Förlin Lars 1950)"

Sökning: WFRF:(Förlin Lars 1950 ) > Göteborgs universitet > Förlin Lars 1950

  • Resultat 11-20 av 164
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
11.
  • Albertsson, Eva, 1979, et al. (författare)
  • Carbonyl reductase mRNA abundance and enzymatic activity as potential biomarkers of oxidative stress in marine fish
  • 2012
  • Ingår i: Marine Environmental Research. - : Elsevier BV. - 0141-1136. ; 80, s. 56-61
  • Tidskriftsartikel (refereegranskat)abstract
    • Carbonyl reductase (CBR) is an enzyme involved in protection from oxidative stress. In rainbow trout (Oncorhynchus mykiss), the hepatic mRNA abundance of the two isoforms (A and B) is increased after exposure to treated sewage effluents, as well as after exposure with beta-naphthoflavone (beta-NF) and the pro-oxidant paraquat In this study, we show that the same chemicals similarly increase the single known hepatic CBR mRNA level and CBR catalytic activity in the coastal living eelpout (Zoarces viviparus). Hepatic CBR mRNA abundance and catalytic activity were also compared between eelpout collected at contaminated and reference sites on the Swedish west coast, but no differences were observed. In conclusion, CBR is a potential biomarker candidate for monitoring the exposure and effects of AhR agonists and/or pro-oxidants in the marine environment, but more research is needed to investigate temporal regulation as well as dose dependency for different chemicals. The mRNA and enzymatic assays presented in this study provide two additional tools for researchers interested in expanding their biomarker battery. (c) 2012 Elsevier Ltd. All rights reserved.
  •  
12.
  •  
13.
  • Albertsson, Eva, 1979, et al. (författare)
  • Induction of hepatic carbonyl reductase/20beta-hydroxysteroid dehydrogenase mRNA in rainbow trout downstream from sewage treatment works--possible roles of aryl hydrocarbon receptor agonists and oxidative stress.
  • 2010
  • Ingår i: Aquatic toxicology (Amsterdam, Netherlands). - : Elsevier BV. - 1879-1514 .- 0166-445X. ; 97:3, s. 243-9
  • Tidskriftsartikel (refereegranskat)abstract
    • Carbonyl reductase/20beta-hydroxysteroid dehydrogenase (CR/20beta-HSD) serves both as a key enzyme in the gonadal synthesis of maturing-inducing hormone in salmonids, and as an enzyme protecting against certain reactive oxygen species. We have previously shown that mRNA of the hepatic CR/20beta-HSD B isoform is increased in rainbow trout caged downstream from a Swedish sewage treatment plant. Here, we report an increase of both the A as well as B form in fish kept downstream from a second sewage treatment plant. The two mRNAs were also induced in fish hepatoma cells in vitro after exposure to effluent extract. This indicates that the effects observed in vivo could be a direct effect on the liver, i.e. the mRNA induction does not require a signal from any other organ. When fish were exposed in vivo to several effluents treated with more advanced methods (ozone, moving bed biofilm reactor or membrane bioreactor) the expression of hepatic mRNA CR/20beta-HSD A and B was significantly reduced. Their abundance did not parallel the reduction of estrogen-responsive transcripts, in agreement with our previous observations that ethinylestradiol is not a potent inducer. Treatment with norethisterone, methyltestosterone or hydrocortisone in vivo did not induce the hepatic CR/20beta-HSD A and B mRNA expression. In contrast, both isoforms were markedly induced by the aryl hydrocarbon receptor agonist beta-naphthoflavone as well as by the pro-oxidant herbicide paraquat. We hypothesize that the induction of CR/20beta-HSD A and B by sewage effluents could be due to anthropogenic contaminants stimulating the aryl hydrocarbon receptor and/or causing oxidative stress.
  •  
14.
  •  
15.
  • Albertsson, Eva, 1979, et al. (författare)
  • Proteomic analyses indicate induction of hepatic carbonyl reductase/20beta-hydroxysteroid dehydrogenase B in rainbow trout exposed to sewage effluent.
  • 2007
  • Ingår i: Ecotoxicology and environmental safety. - : Elsevier BV. - 0147-6513. ; 68:1, s. 33-9
  • Tidskriftsartikel (refereegranskat)abstract
    • Proteomic analyses were performed to identify regulated liver proteins in rainbow trout (Oncorhynchus mykiss) caged upstream and downstream from a sewage treatment works (STW). Two-dimensional gel electrophoresis, image analysis and FT-ICR mass-spectrometry revealed four regulated protein spots. The three down-regulated spots contained betaine aldehyde dehydrogenase, lactate dehydrogenase and an unidentified protein respectively. The only up-regulated spot consisted of both mitochondrial ATP synthase alpha-subunit and carbonyl reductase/20beta-hydroxysteroid dehydrogenase (CR/20beta-HSD). Further studies using quantitative PCR revealed a 13.5-fold induction of CR/20beta-HSD B mRNA following STW effluent exposure. The CR/20beta-HSD B gene was not regulated by 17alpha-ethinylestradiol, suggesting that its induction downstream from the STW is due to other factors than exposure to estrogens. Image analysis was initially performed on four gels from each group. These analyses suggested 15 regulated spots. However, validation of the 15 spots by increasing the number of replicates confirmed only four regulated spots. Hence, the present study also demonstrates the need for sufficient biological/technical replication in the interpretation of proteomic data.
  •  
16.
  •  
17.
  •  
18.
  • Asker, Noomi, 1968, et al. (författare)
  • A gene to organism approach-assessing the impact of environmental pollution in eelpout (Zoarces viviparus) females and larvae
  • 2015
  • Ingår i: Environmental Toxicology and Chemistry. - : Wiley. - 0730-7268 .- 1552-8618. ; 34:7, s. 1511-1523
  • Tidskriftsartikel (refereegranskat)abstract
    • A broad biomarker approach was applied to study the effects of marine pollution along the Swedish west coast using the teleost eelpout (Zoarces viviparus) as the sentinel species. Measurements were performed on different biological levels, from the molecular to the organismal, including measurements of messenger RNA (mRNA), proteins, cellular and tissue changes, and reproductive success. Results revealed that eelpout captured in Stenungsund had significantly higher hepatic ethoxyresorufin O-deethylase activity, high levels of both cytochrome P4501A and diablo homolog mRNA, and high prevalence of dead larvae and nuclear damage in erythrocytes. Eelpout collected in Göteborg harbor displayed extensive macrovesicular steatosis, whereby the majority of hepatocytes were affected throughout the liver, which could indicate an effect on lipid metabolism. Results also indicate that eelpouts collected at polluted sites might have an affected immune system, with lower mRNA expression of genes involved in the innate immune system and a higher number of lymphocytes. Biomarker assessment also was performed on livers dissected from unborn eelpout larvae collected from the ovary of the females. No significant differences were noted, which might indicate that the larvae to some extent are protected from effects of environmental pollutants. In conclusion, usage of the selected set of biological markers, covering responses from gene to organism, has demonstrated site-specific biomarker patterns that provided a broad and comprehensive picture of the impact of environmental stressors.
  •  
19.
  • Asker, Noomi, 1968, et al. (författare)
  • Hepatic transcriptome profiling indicates differential mRNA expression of apoptosis and immune related genes in eelpout (Zoarces viviparus) caught at Göteborg harbor, Sweden
  • 2013
  • Ingår i: Aquatic Toxicology. - : Elsevier BV. - 0166-445X .- 1879-1514. ; 130-131, s. 58-67
  • Tidskriftsartikel (refereegranskat)abstract
    • The physiology and reproductive performance of eelpout (Zoarces viviparus) have been monitored along the Swedish coast for more than three decades. In this study, transcriptomic profiling was applied for the first time as an exploratory tool to search for new potential candidate biomarkers and to investigate possible stress responses in fish collected from a chronically polluted area. An oligonucleotide microarray with more than 15,000 sequences was used to assess differentially expressed hepatic mRNA levels in female eelpout collected from the contaminated area at Göteborg harbor compared to fish from a national reference site, Fjällbacka. Genes involved in apoptosis and DNA damage (e.g., SMAC/diablo homolog and DDIT4/DNA-damage-inducible protein transcript 4) had higher mRNA expression levels in eelpout from the harbor compared to the reference site, whereas mRNA expression of genes involved in the innate immune system (e.g., complement components and hepcidin) and protein transport/folding (e.g., signal recognition particle and protein disulfide-isomerase) were expressed at lower levels. Gene Ontology enrichment analysis revealed that genes involved biological processes associated with protein folding, immune responses and complement activation were differentially expressed in the harbor eelpout compared to the reference site. The differential mRNA expression of selected genes involved in apoptosis/DNA damage and in the innate immune system was verified by quantitative PCR, using the same fish in addition to eelpout captured four years later. Thus, our approach has identified new potential biomarkers of pollutant exposure and has generated hypotheses on disturbed physiological processes in eelpout. Despite a higher mRNA expression of genes related to apoptosis (e.g., diablo homolog) in eelpout captured in the harbor there were no significant differences in the number of TUNEL-positive apoptotic cells between sites. The mRNA level of genes involved in apoptosis/DNA damage and the status of the innate immune system in fish species captured in polluted environments should be studied in more detail to lay the groundwork for future biomonitoring studies.
  •  
20.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 11-20 av 164
Typ av publikation
tidskriftsartikel (96)
konferensbidrag (31)
rapport (17)
annan publikation (7)
forskningsöversikt (7)
bokkapitel (6)
visa fler...
visa färre...
Typ av innehåll
refereegranskat (107)
övrigt vetenskapligt/konstnärligt (57)
Författare/redaktör
Larsson, Åke, 1944 (52)
Larsson, D. G. Joaki ... (45)
Parkkonen, Jari, 195 ... (45)
Sturve, Joachim, 196 ... (30)
Hanson, Niklas, 1976 (24)
visa fler...
Kristiansson, Erik, ... (16)
Asker, Noomi, 1968 (16)
Olsson, Jens (14)
Albertsson, Eva, 197 ... (13)
Lennquist, Anna, 197 ... (12)
Bignert, A. (12)
Carney Almroth, Beth ... (11)
Gunnarsson, Lina-Mar ... (10)
Kling, Peter, 1968 (9)
Sandström, Olof (9)
Nyberg, Elisabeth (8)
Olsson, J. (7)
Franzén, Fredrik (7)
Mustamäki, Noora (7)
Landner, Lars (6)
Gercken, J. (6)
Fick, Jerker (5)
Adolfsson-Erici, M. (5)
Ek, Caroline (5)
Balk, Lennart (5)
Ericson, Y. (5)
Bignert, Anders (5)
Danielsson, Sara (5)
Granmo, Åke, 1943 (5)
Karlsson, M (4)
Heimbrand, Yvette (4)
Asplund, Lillemor (4)
Ericson, Ylva (4)
Celander, Malin C., ... (4)
Balk, L. (4)
Danielsson, S (4)
Dave, Göran, 1945 (3)
Adolfsson-Erici, Mar ... (3)
Andersson, Jan (3)
Appelberg, Magnus (3)
Norrgren, L (3)
Norrgren, Leif (3)
Birgersson, Göran (3)
Hilvarsson, Annelie, ... (3)
Frenzilli, G. (3)
Broeg, K. (3)
Barsiene, J. (3)
Schiedek, D. (3)
Ekelund, Rolf (3)
visa färre...
Lärosäte
Chalmers tekniska högskola (17)
Stockholms universitet (7)
Umeå universitet (4)
Kungliga Tekniska Högskolan (2)
Sveriges Lantbruksuniversitet (2)
visa fler...
Lunds universitet (1)
Naturvårdsverket (1)
Havs- och vattenmyndigheten (1)
visa färre...
Språk
Engelska (120)
Svenska (44)
Forskningsämne (UKÄ/SCB)
Naturvetenskap (152)
Medicin och hälsovetenskap (18)
Teknik (7)
Lantbruksvetenskap (3)

År

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy