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Träfflista för sökning "WFRF:(Larsson Lars) ;pers:(Kristiansson Erik 1978);pers:(Larsson D. G. Joakim 1969)"

Sökning: WFRF:(Larsson Lars) > Kristiansson Erik 1978 > Larsson D. G. Joakim 1969

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11.
  • Kristiansson, Erik, 1978, et al. (författare)
  • Characterization of the Zoarces viviparus liver transcriptome using massively parallel pyrosequencing
  • 2009
  • Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 10:345
  • Tidskriftsartikel (refereegranskat)abstract
    • Background The teleost Zoarces viviparus (eelpout) lives along the coasts of Northern Europe and has long been an established model organism for marine ecology and environmental monitoring. The scarce information about this species genome has however restrained the use of efficient molecular-level assays, such as gene expression microarrays. Results In the present study we present the first comprehensive characterization of the Zoarces viviparus liver transcriptome. From 400,000 reads generated by massively parallel pyrosequencing, more than 50,000 pieces of putative transcripts were assembled, annotated and functionally classified. The data was estimated to cover roughly 40% of the total transcriptome and homologues for about half of the genes of Gasterosteus aculeatus (stickleback) were identified. The sequence data was consequently used to design an oligonucleotide microarray for large-scale gene expression analysis. Conclusion Our results show that one run using a Genome Sequencer FLX from 454 Life Science/Roche generates enough genomic information for adequate de novo assembly of a large number of genes in a higher vertebrate. The generated sequence data, including the validated microarray probes, are publicly available to promote genome-wide research in Zoarces viviparus.
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16.
  • Lennquist, Anna, 1978, et al. (författare)
  • Physiology and mRNA expression in rainbow trout (Oncorhynchus mykiss) after long-term exposure to the new antifoulant medetomidine.
  • 2011
  • Ingår i: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. - : Elsevier BV. - 1532-0456. ; 154:3, s. 234-41
  • Tidskriftsartikel (refereegranskat)abstract
    • Medetomidine is under evaluation for use as an antifouling agent, and its effects on non-target aquatic organisms are therefore of interest. In this study, rainbow trout was exposed to low (0.5 and 5.0nM) concentrations of medetomidine for up to 54days. Recently we have reported on effects on paleness and melanophore aggregation of medetomidine in these fish. Here, specific growth rates were investigated together with a broad set of physiological parameters including plasma levels of growth hormone (GH), insulin-like growth factor-I (IGF-I) and leptin, glucose and haemoglobin (Hb), hematocrit (Ht), condition factor, liver and heart somatic indexes (LSI, HSI). Hepatic enzyme activities of CYP1A (EROD activity), glutathione S-transferases (GST) and glutathione reductase (GR) were also measured. Additionally, hepatic mRNA expression was analysed through microarray and quantitative PCR in fish sampled after 31days of exposure. Medetomidine at both concentrations significantly lowered blood glucose levels and the higher concentration significantly reduced the LSI. The mRNA expression analysis revealed few differentially expressed genes in the liver and the false discovery rate was high. Taken together, the results suggest that medetomidine at investigated concentrations could interfere with carbohydrate metabolism of exposed fish but without any clear consequences for growth.
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  • Resultat 11-16 av 16

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