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Träfflista för sökning "L773:0095 1137 srt2:(1995-1999)"

Sökning: L773:0095 1137 > (1995-1999)

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  • Albert, MJ, et al. (författare)
  • Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR
  • 1997
  • Ingår i: Journal of clinical microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 35:6, s. 1633-1635
  • Tidskriftsartikel (refereegranskat)abstract
    • In a previous study using pure bacterial cultures in a PCR assay, a primer pair corresponding to a unique chromosomal region of Vibrio cholerae O139 Bengal generated an amplicon from only V. cholerae O139 Bengal. PCR with the same primer pair was used to screen 180 diarrheal stool specimens. All the 67 V. cholerae O139 culture-positive stool specimens were positive by PCR, and the remaining specimens, which contained either other recognized enteric pathogens or no pathogens, were all negative by PCR.
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  • Boman, Jens, 1957-, et al. (författare)
  • Failure to detect Chlamydia trachomatis in cell culture by using a monoclonal antibody directed against the major outer membrane protein
  • 1997
  • Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 35:10, s. 2679-2680
  • Tidskriftsartikel (refereegranskat)abstract
    • Two commercially available monoclonal antibodies for cell culture confirmation of Chlamydia trachomatis were compared in two prospective studies and one large retrospective study. In total, more than 33,000 genital specimens were cultured in parallel and stained with both antibodies, one of which was directed against the major outer membrane protein (MOMP) and one uf which was directed against the lipopolysaccharide (LPS). We found the anti-LPS-based assay to be more sensitive and as specific as the anti-MOMP-based assay for C. trachomatis cell culture confirmation of genital specimens.
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  • Bunikis, J, et al. (författare)
  • Molecular polymorphism of the Lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic
  • 1996
  • Ingår i: Journal of Clinical Microbiology. - Washington, DC, United States : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 34:2, s. 364-368
  • Tidskriftsartikel (refereegranskat)abstract
    • Lyme disease Borrelia species are distributed in temperate areas of North America and Eurasia. To elucidate the distribution of borreliae in subarctic regions, strains isolated from Ixodes ricinus and Ixodes uriae ticks found on islands in the northern Atlantic and Baltic Sea were molecularly characterized. All isolates were verified as Borrelia garinii by 16S rRNA gene analysis and immunoblotting with monoclonal antibodies specific for the outer surface proteins A and C. Three ribotypes (RTs) of B. garinii were delineated. I. ricinus complex-associated RT1 was phenotypically most heterogeneous. Two newly identified ribotypes were shared by different tick species and conformed to two established OspA serotypes. RT2 was restricted to the islands in the northern Baltic Sea, whereas RT3 was recovered also from ticks found in the North Atlantic. In conclusion, molecular polymorphism of the studied borrelia isolates suggests a complex enzootic potential of B. garinii in northern Europe and implies a novel, seabird tick I. uriae-associated enzootic focus of Lyme disease borreliae in the North Atlantic. 
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  • Resultat 1-10 av 67

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