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Träfflista för sökning "WAKA:kon ;lar1:(hb);srt2:(1990-1994)"

Sökning: WAKA:kon > Högskolan i Borås > (1990-1994)

  • Resultat 1-7 av 7
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1.
  • Block, K, et al. (författare)
  • Characterization of a minichromosome derived from the transposing element TE1 in Drosophila melanogaster
  • 1991
  • Ingår i: Hereditas. - : Wiley-Blackwell Publishing, Inc.. - 0018-0661. ; , s. 82-83
  • Konferensbidrag (refereegranskat)abstract
    • The transposing element TEI contains the structural genes white and roughest from the Drosophilr X-chromosome. These genes are flanked by FB-elements, which are responsible for the mobility. At one occasion the TE, probably together with an adjacent segment in chromosome 2. has formed a minichromosome. This chromosome contains both the structural genes, the FB-elements,some centromeric and/or telomeric heterochromatin. It probably has a centromere as well, as it is transferred to the offspring at a high rate. From this minichromosome a smaller one has originated, probably through the loss of the region from chromosome 2 and some heterochromatin. This smaller minichromosome has been characterized in the following way: 1. Size determination hy pulsed field gel electrophoresis. -The chromosome turned out to be little more than one megabase. 2. y-irradiation of DNA from the minichromosome. ~ The aim of this experiment is to find out if the chromosome is circular or linear. A radiation dose which causes one break within a circle ought to accumulate DNA of the same size as the minichromosome. In this case no accumulation occurred and thus the chromosome is probably linear. 3. Cloning of sequences from the minichromosome. ~ A low melting agarose gel was run and a fragment was cut out from a region which contained DNA fragments of the same size as the minichromosome. The DNA was cut simultaneously by EcoR I and Pst I and ligated into the vector pBS containing T7 and T3 primers. The ligated DNA was amplified by the PCR method, which rendered several fragments of varying size. These fragments were ligated into the vector pCR1000TM. Positive clones are being analysed at present. With these clones we intend to construct a map of the minichromosome.
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  • Luepker, RV, et al. (författare)
  • Strategies for change : Public education
  • 1991
  • Ingår i: Proceedings of the National Heart, Lung and Blood Institute Symposium on Rapid Identification and Treatment of Acute Myocardial Infarction: Issues and Answers. - : DIANE Publishing Company. - 0788128256 ; , s. 117-121
  • Konferensbidrag (refereegranskat)
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7.
  • Ståhl, Fredrik, et al. (författare)
  • OFAGE analysis of large DNA restriction fragments from multidrug resistant SEWA mouse cells
  • 1990
  • Ingår i: Hereditas. - : Wiley-Blackwell Publishing, Inc.. - 0018-0661. ; , s. 9-
  • Konferensbidrag (refereegranskat)abstract
    • The aim of this study is to detect similarities and differences in the gene amplification process, when MDR cells develop after selection in different drugs. For this purpose, nine independent MDR sublines of the murine SEWA TC13K cell line were established by stepwise selection in actinomycinD( Al,A2,A4),colcemid(Cl,C2,C3),or vincristine (Vl, V3, V4). All MDR sublines displayed numerous DMs. DNA from all lines was digested with EcoRl and the infrequently cutting enzyme SfiI. DNA-fragments from the SfiI-digests were separated by orthogonal field alterating gel electrophoresis (OFAGE). Hybridizations were made with two probes: (1) cp28, a Chinese hamster P-glycoprotein cDNA sequence, kindly supplied by Dr Alexander Van der Bliek, The Netherlands Cancer Institute in Amsterdam; (2) Ie7, a genomic clone from a DM mini-library (Stihl et al., Hereditas 106:97, 1987). The hybridization patterns of the Sfildigests were very similar for both probes, while the patterns of the EcoRI-digests differed considerably. No drugspecific amplification pattern was revealed. The Ie7 probe did not cross-hybridize to the cp28 cDNA-sequence. However, low stringency hybridization of Ie7 to a 4.5 kb mRNA (hamster) has been reported (Diddens et al., Int. J. Cancer 40:635, 1987). Still, the similarities in the hybridization patterns suggest that Ie7 DNA-sequence is located close to the cp28 DNA-sequence, perhaps as a part of an intron. In the SfiI digest the cp28 probe hybridized to seven DNA-fragments (six of which were detected also with Ie7) that were amplified in most of the lines. The same DNAfragments were present but not amplified in the control line TC13K. The presence of several hybridizing fragments also in the control line is probably due to homology within the P-glycoprotein gene family. Other amplified sequences were unique to each cell line and are presumably a result of so-called novel joints. The large number of new hybridizing fragments thus reflects a recombinational process during amplicon formation. The presence of specific cytogenetic markers in some of the cell lines indicated that the MDR cells were of monoclonal origin. Therefore, it is possible that each line contains a homogeneous population of DMs, each contributing the same complex pattern of novel fragments in the SfiI analysis. Another explanation of this complexity would be thai different amplicons (with different novel joints) are located on different DMs in a heterogeneous DM population.
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  • Resultat 1-7 av 7
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