| 1. |
- Jansson, Henrik, et al.
(författare)
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Analysis of the interleukin-1 and interleukin-6 polymorphisms in patients with chronic periodontitis. A pilot study
- 2006
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Ingår i: Swedish Dental Journal. - 0347-9994. ; 30:1, s. 17-23
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to analyse whether the interleukin-1 (IL-1) and IL-6 gene polymorphisms were associated with the susceptibility of chronic periodontitis. Genomic DNA was obtained from 20 patients with chronic periodontitis and 31 periodontally healthy subjects. All subjects were of North European heritage. The test subjects were kept in a maintenance program after periodontal treatment but yet showing signs of recurrent disease. Genotyping of the IL-1 alpha[+4845C>T], IL-1 beta [-3954C>T] and IL-6 [-174G>C] polymorphisms was carried out using an allelic discrimination Assay-by-Design method on ABI PRISM 7900 Sequence Detection System. All genotypes were analyzed using the GeneMapper 2.0 software. A similar distribution of Single Nucleotide Polymorphism (SNP) was seen in both groups. Analysis by logistic regression including gender, IL-1 alpha [+4845C>T], IL-1 beta [-3954C>T], IL-6 [-174G>C] genotypes, the composite IL-1 genotype, the combination of the composite IL-1 genotype and the IL-6 -174G>C genotype and adjusting for smoking did not result in any statistically significant difference. SNPs in IL-1 alpha[+4845C>T], IL-1 beta [-3954C>T] and IL-6 [-174G>C] do not seem to increase the susceptibility to chronic periodontitis in this group of subjects.
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| 2. |
- Jansson, Henrik, et al.
(författare)
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The Microbial Outcome Observed with Polymerase Chain Reaction in Subjects with Recurrent Periodontal Disease following local treatment with 25% metronidazole gel
- 2004
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Ingår i: Swedish Dental Journal. - 0347-9994. ; 28:2, s. 67-76
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this study was to evaluate the microbial outcome in patients with recurrent periodontal disease following treatment with 25% metronidazole gel using the polymerase chain reaction (PCR). Twenty subjects in a maintenance care program but with recurrent periodontal disease participated. Three months after scaling and root planing a total of 40 sites, 2 in each patient, with pocket probing depth of > or = 5 mm were selected. One site randomly selected was treated with 25% metronidazole gel (test) and the other site with a placebo gel (control). A bacterial sample was collected on paperpoint from each test and control site at baseline and 12 weeks after treatment. The following pathogens were analysed and detected with PCR:Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.) and Prevotella nigrescens (P.n.). At baseline, A.a., P.g. and P.n. were detected in 30, 60 and 70% of all test sites and in 32, 58 and 21% of all control sites. There was a statistically significant difference between the test and control sites for P.n. at baseline. The major difference after treatment with 25% metronidazole gel was the increase of positive control sites for P.g. and P.n. However, there were no statistically significant differences in the occurrence rate of A.a., P.g. and P.n. at test and control sites after treatment. This study has shown that 25% metronidazole gel treatment did not seem to influence the microbial outcome, when PCR was used to analyse the presence/absence of A.a., P.g. and P.n. in this group of subjects with recurrent periodontal disease.
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| 3. |
- Sonesson, Mikael, et al.
(författare)
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Salivary IgA in minor-gland saliva of children, adolescents, and young adults
- 2010
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Ingår i: Swedish Dental Journal. - 0347-9994. ; 34:4, s. 236-236
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Immunoglobulin A (IgA) is the principal immunoglobulin in human saliva and minor salivary glands contribute with approximately 30-35 % of the total salivary IgA in whole saliva. Salivary IgA is considered to be a vital component of the defence of the oral tissues and the concentration seems to increase with age. However, data on the salivary IgA-concentration in minor gland saliva of children, compared to adults, is not available. Objectives: To study the salivary IgA-concentration in minor gland saliva in defined mucosal areas, and in un-stimulated whole saliva, in children in different ages and young adults. Methods: 90 individuals were recruited: 3-year-old children (n = 30), 14-year-old children (n = 30), and young adults (n = 30). Minor gland saliva was collected on labial and buccal mucosa with filter papers and the volume was determined using a Periotron 8000. The whole saliva was collected by draining into a tube. Assessment of the salivary IgA-concentration was carried out in a sandwich ELISA. One-way ANOVA was used testing the differences between the age-groups (at the 0.05 level of significance). Results: The salivary IgA-concentration in labial gland saliva was significantly lower (p<0.05) in the 3-year-olds (3.7 mg/100 ml +/- 3.5) compared with the 14-year-olds (12.6 mg/100ml +/- 12.8) and the adults (12.8 mg/100ml +/- 13.4). In the un-stimulated whole saliva, a significantly lower salivary IgA-concentration was noted for the youngest group (9.0 mg/100ml +/-9.1) compared with the 14-year-olds (17.9 mg/100ml +/-14.9) and the adults (17.0 mg/100ml +/-9.9). Conclusion: The salivary IgA-concentration in labial and un-stimulated whole saliva seems to increase with age. This may reflect a developing specific immune response in saliva of young children. Approved by the ethical committee, Lund University. Funded by The Crafoord foundation (20090733), Swedish Patent Revenue Foundation and Faculty of Odontology, Malmö University (OD27-2009/275).
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| 4. |
- Wallin-Bengtsson, Viveca, et al.
(författare)
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Alpha-1-antitrypsin deficiency and periodontitis, a pilot study
- 2011
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Ingår i: Swedish Dental Journal. - 0347-9994. ; 35:1, s. 33-40
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to investigate if periodontal parameters and elastase in gingival crevicular fluid (GCF) are different in alpha-1-antitrypsin deficient (AATD) subjects compared to subjects with normal AAT level. Thirty subjects were included, 20 of whom with severe AATD, phenotype PiZZ. Ten AATD subjects suffered from chronic obstructive pulmonary disease (COPD, group 1) and 10 were asymptomatic (group 2). Ten control subjects, phenotype PiMM, (group 3) were recruited from a public dental clinic. The examination comprised of sampling of GCF, Gingival Index (GI), Plaque Index (PII), probing pocket depth (PPD) and radiography. GCF was collected with paper strips (Periopaper (R)). Plasma AAT concentration was measured by nephelometry and AAT in GCF with ELISA. Elastase activity and protein in GCF were determined by spectrophotometry. The mean values for GI, PII, PPD and the radiological measurements did not show any statistically significant differences between the groups. AAT in plasma and GCF demonstrated very low values in groups 1 and 2 with no significant difference between these groups but a statistical difference in comparison with group 3. Elastase in GCF did not show any difference between the three groups. In conclusion, neither the periodontal parameters nor the elastase in GCF were different in AATD subjects, phenotype PiZZ, when compared to subjects with normal AAT level, phenotype PiMM, in this material.
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