| 971. |
- Gremel, Gabriela, et al.
(författare)
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A systematic analysis of commonly used antibodies in cancer diagnostics
- 2014
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Ingår i: Histopathology. - : Wiley. - 0309-0167 .- 1365-2559. ; 64:2, s. 293-305
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Tidskriftsartikel (refereegranskat)abstract
- AimsImmunohistochemistry plays a pivotal role in cancer differential diagnostics. To identify the primary tumour from a metastasis specimen remains a significant challenge, despite the availability of an increasing number of antibodies. The aim of the present study was to provide evidence-based data on the diagnostic power of antibodies used frequently for clinical differential diagnostics. Methods and resultsA tissue microarray cohort comprising 940 tumour samples, of which 502 were metastatic lesions, representing tumours from 18 different organs and four non-localized cancer types, was analysed using immunohistochemistry with 27 well-established antibodies used in clinical differential diagnostics. Few antibodies, e.g. prostate-specific antigen and thyroglobulin, showed a cancer type-related sensitivity and specificity of more than 95%. A majority of the antibodies showed a low degree of sensitivity and specificity for defined cancer types. Combinations of antibodies provided limited added value for differential diagnostics of cancer types. ConclusionsThe results from analysing 27 diagnostic antibodies on consecutive sections of 940 defined tumours provide a unique repository of data that can empower a more optimal use of clinical immunohistochemistry. Our results highlight the benefit of immunohistochemistry and the unmet need for novel markers to improve differential diagnostics of cancer.
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| 972. |
- Grespi, A., et al.
(författare)
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Probing the electrode-liquid interface using operando total-reflection X-ray absorption spectroscopy
- 2024
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Ingår i: Surface Science. - : Elsevier B.V.. - 0039-6028 .- 1879-2758. ; 748
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Tidskriftsartikel (refereegranskat)abstract
- Traditional methods to study electrochemical (EC) processes, although successful, are based on current/voltage measurements, providing information about performances rather than offering a direct observation of chemical and structural changes occurring at the electrode surface. These processes are localized at the electrode-electrolyte interface, the structure of which is the main determinant of their behavior, but most surface sensitive experimental techniques are limited to ex situ conditions, owing to the need for an ultra-high vacuum environment. In this contribution, we report operando X-ray absorption spectroscopy in total external reflection geometry (Refle-XAFS) at P64 beamline (DESY, Hamburg), using a simple and versatile EC flow cell designed for multimodal surface sensitive studies with hard X-ray scattering and spectroscopy techniques. We show that the Refle-XAFS method can be used to study chemical surface changes of industrial alloys and model electrodes in harsh electrochemical environments, without being limited to thin film samples. The surface passive film development and breakdown of a corrosion-resistant Ni-Cr-Mo alloy and the electro-oxidation of polycrystalline gold (poly-Au), relevant for fundamental studies on water electrolysis, were investigated. Despite the strong attenuation of the beam by the electrolyte and the PEEK walls of the EC cell, nanoscale surface oxide films were detected using beam energies down to 8 keV. The passivity breakdown region of Ni alloy 59 in 1 M NaCl at pH 7 and pH 12 was identified, showing differences in the composition of the surface oxides during anodic polarization. The electro-oxidation of poly-Au in 0.05 M H2SO4 was observed, showing a progression from two-dimensional Au1+/3+ to three-dimensional thick Au3+ surface oxide/hydroxide during OER.
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| 973. |
- Grosshans, Holger, et al.
(författare)
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Computational sensitivity study of spray dispersion and mixing on the fuel properties in a gas turbine combustor
- 2017
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Ingår i: Fluid Dynamics Research. - : Institute of Physics Publishing (IOPP). - 0169-5983 .- 1873-7005. ; 49:2
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Tidskriftsartikel (refereegranskat)abstract
- A swirl stabilized gas turbine burner has been simulated in order to assess the effects of the fuel properties on spray dispersion and fuel-air mixing. The properties under consideration include fuel surface tension, viscosity and density. The turbulence of the gas phase is modeled applying the methodology of large eddy simulation whereas the dispersed liquid phase is described by Lagrangian particle tracking. The exchange of mass, momentum and energy between the two phases is accounted for by two-way coupling. Bag and stripping breakup regimes are considered for secondary droplet breakup, using the Reitz-Diwakar and the Taylor analogy breakup models. Moreover, a model for droplet evaporation is included. The results reveal a high sensitivity of the spray structure to variations of all investigated parameters. In particular, a decrease in the surface tension or the fuel viscosity, or an increase in the fuel density, lead to less stable liquid structures. As a consequence, smaller droplets are generated and the overall spray surface area increases, leading to faster evaporation and mixing. Furthermore, with the trajectories of the small droplets being strongly influenced by aerodynamic forces (and less by their own inertia), the spray is more affected by the turbulent structures of the gaseous phase and the spray dispersion is enhanced.
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| 974. |
- Grosshans, Holger, et al.
(författare)
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Enhanced liquid-gas mixing due to pulsating injection
- 2015
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Ingår i: Computers & Fluids. - : Elsevier BV. - 0045-7930 .- 1879-0747. ; 107, s. 196-204
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Tidskriftsartikel (refereegranskat)abstract
- This paper considers the effects of intermittent injection of a liquid jet or spray on the initial break-up and mixing of one fluid with the surrounding ambient fluid. The aim of the analysis is to describe the physical process and indicate the mechanisms that control the mixing under different flow conditions (time-dependent injection and its frequency relative to the time scales of the flow) and fluid properties (density ratio), Schmidt number for a single phase case which is studied for comparison, or the Weber number for the two-phase cases. The computations use Large Eddy Simulation (LES) to account for turbulence, and either Volume Of Fluid (VOF) for the initial break-up or Lagrangian Particle Tracking (LPT) with droplet break-up model in the case of liquid droplets injected into the ambient gas. The results show that, depending on the physical properties of the liquid and ambient gas, the initial break-up and turbulent mixing can be enhanced substantially with intermittent injection. The numerical modeling is validated by recovering key results of experimental and analytical works. It can be observed that a main effect during the mixing is the suction of ambient fluid at the tail of the injected liquid, which depends on the fluid properties. Increased injection frequency shows to increase the mixing significantly during the initial transient phase.
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| 975. |
- Grosshans, H., et al.
(författare)
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Sensitivity of VOF simulations of the liquid jet breakup to physical and numerical parameters
- 2016
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Ingår i: Computers & Fluids. - : Elsevier. - 0045-7930 .- 1879-0747. ; 136, s. 312-323
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Tidskriftsartikel (refereegranskat)abstract
- In this paper the characteristics of the primary breakup of a liquid jet is analyzed numerically. We applied the Volumes of Fluids (VOF) approach utilizing the Direction Averaged Curvature (DAC) model, to estimate the interface curvature, and the Direction Averaged Normal (DAN) model, to propagate the interface. While being used for the first time to predict liquid atomization, this methodology showed a high accuracy. The influence of varying the fluid properties, namely liquid-gas density and viscosity ratio, and injection conditions is discussed related to the required grid resolution. Resulting droplet sizes are compared to distributions obtained through the One-Dimensional Turbulence (ODT) model.
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| 976. |
- Grønberg, Christina, et al.
(författare)
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Membrane Anchoring and Ion-Entry Dynamics in P-type ATPase Copper Transport
- 2016
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Ingår i: Biophysical Journal. - : Elsevier. - 0006-3495 .- 1542-0086. ; 111:11, s. 2417-2429
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Tidskriftsartikel (refereegranskat)abstract
- Cu+-specific P-type ATPase membrane protein transporters regulate cellular copper levels. The lack of crystal structures in Cu+-binding states has limited our understanding of how ion entry and binding are achieved. Here, we characterize the molecular basis of Cu+ entry using molecular-dynamics simulations, structural modeling, and in vitro and in vivo functional assays. Protein structural rearrangements resulting in the exposure of positive charges to bulk solvent rather than to lipid phosphates indicate a direct molecular role of the putative docking platform in Cu+ delivery. Mutational analyses and simulations in the presence and absence of Cu+ predict that the ion-entry path involves two ion-binding sites: one transient Met148-Cys382 site and one intramembranous site formed by trigonal coordination to Cys384, Asn689, and Met717. The results reconcile earlier biochemical and x-ray absorption data and provide a molecular understanding of ion entry in Cu+-transporting P-type ATPases.
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| 977. |
- Grönstedt, Tomas, 1970, et al.
(författare)
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Multidisciplinary assessment of a year 2035 turbofan propulsion system
- 2022
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Ingår i: 33rd Congress of the International Council of the Aeronautical Sciences, ICAS 2022. - : International Council of the Aeronautical Sciences. - 9781713871163 ; 7, s. 4981-4990
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Konferensbidrag (refereegranskat)abstract
- A conceptual design of a year 2035 turbofan is developed and integrated onto a year 2035 aircraft model. The mission performance is evaluated for CO2, noise and NOx and is compared with a notional XWB/A350-model. An OGV heat exchanger is then studied rejecting heat from an electric generator, and its top-level performance is evaluated. The fan, the booster and the low-pressure turbine of the propulsion system are subject to more detailed aero design based on using commercial design tools and CFD-optimization. Booster aerodynamic modelling output is introduced back into the performance model to study the integrated performance of the component. The top-level performance aircraft improvements are compared to top-level-trends and ICAO estimates of technology progress potential, attempting to evaluate whether there is some additional margin for efficiency improvement beyond the ICAO technology predictions for the same time frame.
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| 978. |
- Guanciale, Roberto, et al.
(författare)
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SoK : Confidential Quartet - Comparison of Platforms for Virtualization-Based Confidential Computing
- 2022
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Ingår i: Proceedings - 2022 IEEE International Symposium on Secure and Private Execution Environment Design, SEED 2022. - : Institute of Electrical and Electronics Engineers Inc.. - 9781665485265 - 9781665485272 ; , s. 109-120
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Konferensbidrag (refereegranskat)abstract
- Confidential computing allows processing sensitive workloads in securely isolated spaces. Following earlier adoption of process-based approaches to isolation, vendors are now enabling hardware and firmware support for virtualization-based confidential computing on several server platforms. Due to variations in the technology stack, threat model, implementation and functionality, the available solutions offer somewhat different capabilities, trade-offs and security guarantees. In this paper we review, compare and contextualize four virtualization-based confidential computing technologies for enterprise server platforms - AMD SEV, ARM CCA, IBM PEF and Intel TDX.
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| 979. |
- Gudmarsson, Anders, 1983-, et al.
(författare)
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Application of resonant acoustic spectroscopy to asphalt concrete beams for determination of the dynamic modulus
- 2012
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Ingår i: Materials and Structures. - : Springer Netherlands. - 1359-5997 .- 1871-6873. ; 45:12, s. 1903-1913
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, a new application of resonant acoustic spectroscopy (RAS) is examined for constructing asphalt concrete mastercurves from seismic testing. The frequency-dependent material properties can be characterized from multiple modes of vibration through the use of RAS. Beam-shaped asphalt specimens are tested at multiple temperatures to determine the resonance frequencies of the specimens. The resonance frequencies are estimated by applying a small load impulse and measuring the resulting acceleration through the specimens. Using RAS, the material properties of the specimens are determined numerically using the measured resonance frequencies. The results presented show that the frequency-dependent dynamic modulus of the asphalt concrete specimens can be characterized using several modes of vibration at each testing temperature.
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| 980. |
- Gudmundsdottir, Valborg, et al.
(författare)
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Whole blood co-expression modules associate with metabolic traits and type 2 diabetes : an IMI-DIRECT study
- 2020
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Ingår i: Genome Medicine. - : BioMed Central. - 1756-994X. ; 12:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: The rising prevalence of type 2 diabetes (T2D) poses a major global challenge. It remains unresolved to what extent transcriptomic signatures of metabolic dysregulation and T2D can be observed in easily accessible tissues such as blood. Additionally, large-scale human studies are required to further our understanding of the putative inflammatory component of insulin resistance and T2D. Here we used transcriptomics data from individuals with (n = 789) and without (n = 2127) T2D from the IMI-DIRECT cohorts to describe the co-expression structure of whole blood that mainly reflects processes and cell types of the immune system, and how it relates to metabolically relevant clinical traits and T2D.Methods: Clusters of co-expressed genes were identified in the non-diabetic IMI-DIRECT cohort and evaluated with regard to stability, as well as preservation and rewiring in the cohort of individuals with T2D. We performed functional and immune cell signature enrichment analyses, and a genome-wide association study to describe the genetic regulation of the modules. Phenotypic and trans-omics associations of the transcriptomic modules were investigated across both IMI-DIRECT cohorts.Results: We identified 55 whole blood co-expression modules, some of which clustered in larger super-modules. We identified a large number of associations between these transcriptomic modules and measures of insulin action and glucose tolerance. Some of the metabolically linked modules reflect neutrophil-lymphocyte ratio in blood while others are independent of white blood cell estimates, including a module of genes encoding neutrophil granule proteins with antibacterial properties for which the strongest associations with clinical traits and T2D status were observed. Through the integration of genetic and multi-omics data, we provide a holistic view of the regulation and molecular context of whole blood transcriptomic modules. We furthermore identified an overlap between genetic signals for T2D and co-expression modules involved in type II interferon signaling.Conclusions: Our results offer a large-scale map of whole blood transcriptomic modules in the context of metabolic disease and point to novel biological candidates for future studies related to T2D.
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