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- Guron, Gregor, 1967, et al.
(författare)
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Cardiac insulin-like growth factor I and growth hormone receptor expression in renal hypertension
- 1996
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Ingår i: Hypertension. - 0194-911X .- 1524-4563. ; 27:3/Pt 2, s. 636-42
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the present study was to investigate the role of insulin-like growth factor I in the development of cardiac hypertrophy in two-kidney, one clip hypertension by relating growth hormone receptor and insulin-like growth factor I receptor mRNA levels to insulin-like growth factor I gene transcription using a solution hybridization/RNase protection assay. Two-kidney, one clip hypertension was induced in male Wistar rats, and experiments were performed 2, 4, 7, and 12 days after surgery. Systolic blood pressure was elevated 2, 7, and 12 days after clipping (P < .001). Left ventricular weights were increased 2, 4, 7, and 12 days after surgery (P < .01). Associated with the rise in blood pressure, left ventricular insulin-like growth factor I mRNA was increased 2, 7, and 12 days after surgery (P < .01). Furthermore, growth hormone receptor and insulin-like growth factor I receptor gene expression increased specifically in the left ventricle of renal hypertensive rats (P < .05 and P < .001, respectively). Left ventricular growth hormone receptor mRNA peaked 7 days after induction of renal artery stenosis. These results show that insulin-like growth factor I, growth hormone receptor, and insulin-like growth factor I receptor mRNA increase in the pressure-overloaded left ventricle of two-kidney, one clip rats, suggesting a role for insulin-like growth factor I and the growth hormone/insulin-like growth factor I axis in the development of cardiac hypertrophy.
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| 2. |
- Wassenius, Ola, et al.
(författare)
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Variability in Skin Exposure in Machine Operators Exposed to Cutting Fluids
- 1998
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Ingår i: Scandinavian Journal of Work, Environment and Health. - : Scandinavian Journal of Work, Environment and Health. - 0355-3140 .- 1795-990X. ; 24:2, s. 125 - 129
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Tidskriftsartikel (refereegranskat)abstract
- Objective. This study describes a new technique for measuring skin exposure to cutting fluids and evaluates the variability of skin exposure among machine operators performing cyclic (repetitive) work. Methods. The technique is based on video recording and subsequent analysis of the videotape by means of computer-synchronized video equipment. The time intervals at which the machine operator's hand was exposed to fluid were registered, and the total wet time of the skin was calculated by assuming different evaporation times for the fluid. The exposure of twelve operators with different work methods was analyzed in six different workshops, which included a range of machine types, from highly automated metal cutting machines (ie, actual cutting and chip removal machines) requiring operator supervision to conventional metal cutting machines, where the operator was required to maneuver the machine and manually exchange products. Results; the relative wet time varied between 0% and 100%. A significant association between short cycle time and high relative wet time was noted. However, there was no relationship between the degree of automatization of the metal cutting machines and wet time. Conclusions. The study shows that skin exposure to cutting fluids can vary considerably between machine operators involved in manufacturing processes using different types of metal cutting machines. The machine type was not associated with dermal wetness. The technique appears to give objective information about dermal wetness. A comment: This publication is a result of a cooperation between the authors at Chalmers University of Technology and Gothenburg University, i.e. between the Department of Transportation and the Department of Occupational Health and the Department of Sociology.
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| 5. |
- Carlsson, Björn, 1958, et al.
(författare)
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Obese (ob) gene defects are rare in human obesity
- 1997
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Ingår i: Obesity Research. - 1071-7323 .- 1550-8528. ; 5:1, s. 30-5
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Tidskriftsartikel (refereegranskat)abstract
- Our knowledge of the role of the recently cloned ob-protein (leptin) in the regulation of body fat stores is largely derived from experiments performed in mice. Different mouse models exhibit abnormalities in ob-gene expression, with extreme overexpression in mice which lack bioactive ob-protein, have nonfunctional ob-receptors or hypothalamic lesions, and undetectable expression in mice with suggested defects in regulatory elements. The aim of this study is to examine if defects, corresponding to those in mice, exist in human obesity. Adipose tissue was obtained from 94 adult obese subjects and from six children who had developed obesity after surgery in the hypothalamic region. Total RNA was isolated and ob-gene expression was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blot. The coding region of the ob-gene was sequenced in both directions in the 94 obese adults. No mutations were detected in the coding region of the ob-gene and ob-gene expression was detectable in all subjects and none of the subjects had an extreme overexpression. There was no systematic increase in ob-expression in obese children with hypothalamic disease compared to their healthy brothers and sisters. These results show that severe abnormalities involving the ob-gene, analogous to those described in mouse models, are rare in human obesity. We therefore conclude that the cloning and subsequent analysis of the ob-gene has not provided information that can, by itself, explain the genetic component in the development of human obesity.
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| 6. |
- Abbas, Abdul-Karim, 1959, et al.
(författare)
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Bicarbonate-sensitive cysteine induced elevation of extracellular aspartate and glutamate in rat hippocampus in vitro
- 1997
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Ingår i: Neurochemistry International. - 0197-0186. ; 30:3, s. 253-259
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Tidskriftsartikel (refereegranskat)abstract
- The effect of different concentrations of cysteine (0.125, 0.25, 0.5 and 1 mM) on the net efflux of endogenous amino acids was studied by the incubation of rat hippocampal slices. Addition of cysteine (1 mM) in bicarbonate containing low K+ medium (5 min) selectively increased the basal net efflux of glutamate and aspartate by 370% and 396%, respectively. High K+ media (50 mM) containing cysteine (1 mM) evoked the net efflux of glutamate and aspartate by 1 454% and 1 019%, respectively. The corresponding effects in control slices without cysteine were 669% and 404%, respectively. No changes were observed on the concentrations of GABA, glutamine and taurine. The cysteine oxidation products, cysteine sulfinate (0.5 μM) and cystine (0.25 mM) were without effects. The effect of cysteine (0.5 mM) was dramatically reduced in media with no added bicarbonate/CO2. Thus, cysteine in a bicarbonate-sensitive manner selectively increases the extracellular concentration of excitotoxic amino acids in adult rat brain in vitro, possibly by interfering with the carrier-mediated glutamate uptake/ release
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| 9. |
- Boström, Ola, 1963, et al.
(författare)
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A new neck injury criterion candidate-based on injury findings in the cervical spinal ganglia after experimental neck extension trauma
- 1996
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Ingår i: PROCEEDINGS OF THE 1996 INTERNATIONAL IRCOBI CONFERENCE ON THE BIOMECHANICS OF IMPACT, SEPTEMBER 11-13, DUBLIN, IRELAND. ; , s. 123-136
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Konferensbidrag (refereegranskat)abstract
- In this study a mathematical model, based on Navier Stokes equations, was developed and validated against experimental data. This model predicts the pressure changes in the spinal canal as a function of the volume change inside the canal during neck bending in the x-z (sagittal) plane. Another aim of the study was to investigate pressure phenomena and ganglion injuries at static neck extension loading and dynamic neck extension trauma with a head-restraint present. Experiments on pigs were conducted. Preliminary results indicate that ganglion injuries, as well as pressure transients inside the spinal canal, seem to correlate to the phase shift when the neck passes an s-shape (or maximal retraction) during the rearward motion of the head. That is, when the upper neck quickly changes from a flexion to an extension shape. Static loading of the neck resulted in no signs of injuries to the ganglia. A possible candidate for a neck injury criterion is presented, based on the relative acceleration between the top and the bottom of the cervical spine. A tolerance level based on the pig tests is also discussed.
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| 10. |
- Johansson Hanse, Jan, 1954, et al.
(författare)
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Sense of Coherence and Ill Health Among the Unemployed and Re-employed After Closure of an Assembly Plant
- 1999
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Ingår i: Work and Stress. - 0267-8373 .- 1464-5335. ; 13:3, s. 204-222
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to investigate the relationship between employment status, ill health and sense of coherence (SOC). A cross-sectional sample of the former workers at the closed down Volvo Kalmar plant in Sweden was used. A questionnaire was mailed to all identified subjects 2 years after the closure of the plant. Responses were obtained from 344 persons, of whom 173were re-employed and 92were unemployed. They answered the questionnaire anonymously on background variables, SOC and ill health (physical and psychological symptoms). A notable difference was found between the re-employed and unemployed, where the unemployed reported symptoms significantly more often as well as a significantly weaker SOC. The main finding in a four-cell model indicated that unemployment had a rather low impact on symptoms in the presence of a strong SOC. Structural equation modelling (SEM) was performed with simultaneous analysis of the unemployed and reemployed. The SEM analysis indicated that SOC seemed to have a greater impact on psychological symptoms among unemployed compared with re-employed persons. However, it should be noted that the present study does not allow for the determination of cause-effect relationships. A comment: This publication is authored together with a senior research competency within psychology with whom Engström have had long time cooperation procedures.
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