| 61. |
- Peleli, Maria, et al.
(author)
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Endothelial-Tumor Cell Interaction in Brain and CNS Malignancies
- 2020
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 21:19
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Research review (peer-reviewed)abstract
- Glioblastoma and other brain or CNS malignancies (like neuroblastoma and medulloblastoma) are difficult to treat and are characterized by excessive vascularization that favors further tumor growth. Since the mean overall survival of these types of diseases is low, the finding of new therapeutic approaches is imperative. In this review, we discuss the importance of the interaction between the endothelium and the tumor cells in brain and CNS malignancies. The different mechanisms of formation of new vessels that supply the tumor with nutrients are discussed. We also describe how the tumor cells (TC) alter the endothelial cell (EC) physiology in a way that favors tumorigenesis. In particular, mechanisms of EC-TC interaction are described such as (a) communication using secreted growth factors (i.e., VEGF, TGF-beta), (b) intercellular communication through gap junctions (i.e., Cx43), and (c) indirect interaction via intermediate cell types (pericytes, astrocytes, neurons, and immune cells). At the signaling level, we outline the role of important mediators, like the gasotransmitter nitric oxide and different types of reactive oxygen species and the systems producing them. Finally, we briefly discuss the current antiangiogenic therapies used against brain and CNS tumors and the potential of new pharmacological interventions that target the EC-TC interaction.
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| 62. |
- Peleli, Maria, et al.
(author)
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Enhanced XOR activity in eNOS-deficient mice Effects on the nitrate-nitrite-NO pathway and ROS homeostasis
- 2016
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In: Free Radical Biology & Medicine. - : Elsevier BV. - 0891-5849 .- 1873-4596. ; 99, s. 472-484
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Journal article (peer-reviewed)abstract
- Xanthine oxidoreductase (XOR) is generally known as the final enzyme in purine metabolism and as a source of reactive oxygen species (ROS). In addition, this enzyme has been suggested to mediate nitric oxide (NO) formation via reduction of inorganic nitrate and nitrite. This NO synthase (NOS)-independent pathway for NO generation is of particular importance during certain conditions when NO bioavailability is diminished due to reduced activity of endothelial NOS (eNOS) or increased oxidative stress, including aging and cardiovascular disease. The exact interplay between NOS- and XOR-derived NO generation is not fully elucidated yet. The aim of the present study was to investigate if eNOS deficiency is associated with changes in XOR expression and activity and the possible impact on nitrite, NO and ROS homeostasis. Plasma levels of nitrate and nitrite were similar between eNOS deficient (eNOS(-/-)) and wildtype (wt) mice. XOR activity was upregulated in eNOS(-/-) compared with wt, but not in nNOS(-/-), iNOS(-/-) or wt mice treated with the non-selective NOS inhibitor L-NAME. Following an acute dose of nitrate, plasma nitrite increased more in eNOS(-/-) compared with wt, and this augmented response was abolished by the selective XOR inhibitor febuxostat. Livers from eNOS(-/-) displayed higher nitrite reducing capacity compared with wt, and this effect was attenuated by febuxostat. Dietary supplementation with nitrate increased XOR expression and activity, but concomitantly reduced superoxide generation. The latter effect was also seen in vitro after nitrite administration. Treatment with febuxostat elevated blood pressure in eNOS(-/-), but not in wt mice. A high dose of dietary nitrate reduced blood pressure in na ve eNOS(-/-) mice, and again this effect was abolished by febuxostat. In conclusion, eNOS deficiency is associated with an upregulation of XOR facilitating the nitrate-nitrite-NO pathway and decreasing the generation of ROS. This interplay between XOR and eNOS is proposed to play a significant role in NO homeostasis and blood pressure regulation.
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| 63. |
- Peleli, Maria, et al.
(author)
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Renal denervation attenuates NADPH oxidase-mediated oxidative stress and hypertension in rats with hydronephrosis
- 2016
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In: American Journal of Physiology - Renal Physiology. - : American Physiological Society. - 0363-6127 .- 1522-1466 .- 1931-857X. ; 310:1, s. F43-F56
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Journal article (peer-reviewed)abstract
- Hydronephrosis is associated with development of salt-sensitive hypertension. Studies suggest that increased sympathetic nerve activity (SNA) and oxidative stress play important roles in renovascular hypertension. This study aimed to investigate the link between renal SNA and NADPH oxidase (NOX) regulation in the development of hypertension in rats with hydronephrosis. Hydronephrosis was induced by partial unilateral ureteral obstruction (PUUO) in young rats. Sham surgery or renal denervation was performed at the same time. Blood pressure was measured during normal, high and low salt diets. Renal excretion pattern, NOX activity and expression, as well as components of RAAS were characterized. On normal salt diet, PUUO rats had elevated blood pressure compared with controls (115±3 vs 87±1 mmHg), and displayed increased urine production and lower urine osmolality. Blood pressure change in response to salt loading (salt-sensitivity) was more pronounced in the PUUO group compared with controls (15±2 vs 5±1mmHg). Renal denervation in PUUO rats attenuated hypertension (97±3mmHg) and salt-sensitivity (5±1mmHg), and normalized renal excretion pattern, whereas the degree of renal fibrosis and inflammation was not changed. NOX activity and expression, as well as renin and AT1A receptor expression, were increased in renal cortex from PUUO rats, and normalized by denervation. Plasma sodium and potassium levels were elevated in PUUO rats and normalized after renal denervation. Denervation in PUUO rats was also associated with reduced NOX expression, superoxide production and fibrosis in the heart. This study emphasizes a link between renal nerves, NOX function, and development of hypertension.
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| 64. |
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| 65. |
- Rasheed, A., et al.
(author)
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Twin-transactions : delayed transaction synchronisation model
- 1998
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In: Object oriented technology. - Berlin, Heidelberg : Encyclopedia of Global Archaeology/Springer Verlag. - 9783540654605 ; , s. 63-
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Conference paper (peer-reviewed)abstract
- In a mobile computing environment, mobile hosts are not available all the time. Due to this reason, mobile computers are usually considered as clients who can connect to different servers and ask for certain services (client-server). To reduce the contention on the narrow bandwidth of the wireless channel, it is suggested to cache part of a database on mobile computers [1]. When a mobile computer also hosts data (or a portion of a data), the data on such hosts will not be available all the time. Replication of such data will make the data available, but will create certain concurrency and consistency problems. The twin-transaction model (TTM) proposes to replicate transaction-processing flow along with data items stored on mobile hosts.
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| 66. |
- Raza, Wasim, et al.
(author)
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A novel microchannel-twisted pinfin hybrid heat sink for hotspot mitigation
- 2024
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In: Applied Thermal Engineering. - : Elsevier BV. - 1359-4311 .- 1873-5606. ; 241
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Journal article (peer-reviewed)abstract
- Thermal hotspots cause excessive localized temperature rise, leading to significant temperature gradients across the microprocessor, which is the primary reason for its inadequate performance and early failure. This study proposes microchannel-pinfin hybrid heat sinks incorporating twisted and non-twisted pinfins of various cross-sectional shapes (hexagonal, pentagonal, square, and triangular) to demonstrate energy-efficient hotspot mitigation in a microprocessor with highly non-uniform power distribution. Microchannels and pinfins are positioned at low- and high-heat-flux zones, respectively, to reduce the temperature variations utilizing their unequal heat transfer capabilities. Here, high- and low-heat-flux zones represent the microprocessor-core area (hotspot) and remaining chip area (background zone), characterized by heat fluxes of 300 W/cm2 and 50 W/cm2, respectively. The pinfin twisting angle varies from 0° to 360° at a step of 45°. Conjugate heat transfer analyses are conducted through numerical solutions of the continuity, Navier-Stokes, and energy equations. The performance of the proposed hybrid heat sinks is compared with the non-hybrid (NH) and hybrid circular pinfins (HCP) heat sinks at Re = 120–440. The hybrid heat sink featuring triangular pinfins twisted at 225° angle (HTP-225) exhibits a remarkable reduction of 48.2 % in the total thermal resistance (Rth) and 58.4 % in the temperature non-uniformity (δT,bs) as compared to the NH heat sinks at Re = 440. Compared to the HCP design, the HTP-225 design shows 26.9 % and 35.8 % lower Rth and δT,bs, respectively. Moreover, the HTP-225 heat sink outperforms the NH heat sink with 46.0 % and 57.0 % lower Rth and δT,bs, respectively, at an equal pumping power of 18.6 mW. Furthermore, the HTP-225 heat sink demonstrates a 96.4 % and 38.5 % higher critical hotspot heat flux dissipating capacity than the NH and HCP heat sinks, respectively, making it a viable and effective solution for alleviating hotspots in high-power-density devices.
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| 67. |
- Raza, Wasif, et al.
(author)
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Air pollution as a risk factor in health impact assessments of a travel mode shift towards cycling
- 2018
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In: Global Health Action. - : Informa UK Limited. - 1654-9716 .- 1654-9880. ; 11:1
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Research review (peer-reviewed)abstract
- Background: Promotion of active commuting provides substantial health and environmental benefits by influencing air pollution, physical activity, accidents, and noise. However, studies evaluating intervention and policies on a mode shift from motorized transport to cycling have estimated health impacts with varying validity and precision. Objective: To review and discuss the estimation of air pollution exposure and its impacts in health impact assessment studies of a shift in transport from cars to bicycles in order to guide future assessments. Methods: A systematic database search of PubMed was done primarily for articles published from January 2000 to May 2016 according to PRISMA guidelines. Results: We identified 18 studies of health impact assessment of change in transport mode. Most studies investigated future hypothetical scenarios of increased cycling. The impact on the general population was estimated using a comparative risk assessment approach in the majority of these studies, whereas some used previously published cost estimates. Air pollution exposure during cycling was estimated based on the ventilation rate, the pollutant concentration, and the trip duration. Most studies employed exposure-response functions from studies comparing background levels of fine particles between cities to estimate the health impacts of local traffic emissions. The effect of air pollution associated with increased cycling contributed small health benefits for the general population, and also only slightly increased risks associated with fine particle exposure among those who shifted to cycling. However, studies calculating health impacts based on exposure-response functions for ozone, black carbon or nitrogen oxides found larger effects attributed to changes in air pollution exposure. Conclusion: A large discrepancy between studies was observed due to different health impact assessment approaches, different assumptions for calculation of inhaled dose and different selection of dose-response functions. This kind of assessments would improve from more holistic approaches using more specific exposure- response functions.
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| 68. |
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| 69. |
- Seidel, Y.E., et al.
(author)
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Mesoscopic mass transport effects in electrocatalytic processes
- 2008
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In: Faraday Discussions. - : Royal Society of Chemistry (RSC). - 1359-6640 .- 1364-5498. ; 140, s. 167-184
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Journal article (peer-reviewed)abstract
- The role of mesoscopic mass transport and re-adsorption effects in electrocatalytic reactions was investigated using the oxygen reduction reaction (ORR) as an example. The electrochemical measurements were performed on structurally well-defined nanostructured model electrodes under controlled transport conditions in a thin-layer flow cell. The electrodes consist of arrays of Pt ultra-microelectrodes (nanodisks) of defined size (diameter similar to 100 nm) separated on a planar glassy carbon (GC) substrate, which were fabricated employing hole-mask colloidal lithography (HCL). The measurements reveal a distinct variation in the ORR selectivity with Pt nanodisk density and with increasing electrolyte flow, showing a pronounced increase of the H2O2 yield, by up to 65%, when increasing the flow rate from 1 to 30 mu L s(-1). These results are compared with previous findings and discussed in terms of a reaction model proposed recently (A. Schneider et al., Phys. Chem. Chem. Phys., 2008, 10, 1931), which includes (i) direct reduction to H2O on the Pt surface and (ii) additional H2O2 formation and desorption on both Pt and carbon surfaces and subsequent partial re-adsorption and further reduction of the H2O2 molecules on the Pt surface. The potential of model studies on structurally defined catalyst surfaces and under well-defined mass transport conditions in combination with simulations for the description of electrocatalytic reactions is discussed.
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| 70. |
- Seidel, Y. E., et al.
(author)
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Nanostructured Pt/GC model electrodes prepared by the deposition of metal-salt-loaded micelles
- 2007
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 23:10, s. 5795-5801
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Journal article (peer-reviewed)abstract
- Novel, nanostructured, carbon-supported Pt model electrodes with homogeneously distributed Pt nanoparticles of uniform size were fabricated and analyzed with respect to their electrochemical properties. For this purpose, Pt-salt-loaded micelles were deposited on a glassy carbon substrate and subsequently exposed to an oxygen plasma and a H-2 atmosphere for removal of the polymer carriers and reduction of the Pt salt. The morphology of the resulting nanoparticles and their electrochemical/electrocatalytic properties were characterized by high-resolution scanning electron microscopy, X-ray photoelectron spectroscopy, cyclic voltammetry, and differential electrochemical mass spectrometry for CO electrooxidation. The data demonstrate that this method is generally suited to the production of nanostructured model electrodes with well-defined and independently adjustable particle size and interparticle distance distributions, which are specifically suited for quantitative studies of transport processes in electrocatalytic reactions.
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