| 1. |
- Almstrand, Ann-Charlotte, et al.
(author)
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Airway monitoring by collection and mass spectrometric analysis of exhaled particles.
- 2009
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In: Analytical chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 81:2, s. 662-8
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Journal article (peer-reviewed)abstract
- We describe a new method for simultaneously collecting particles in exhaled air for subsequent chemical analysis and measuring their size distribution. After forced exhalation, particles were counted and collected in spots on silicon wafers with a cascade impactor. Several phospholipids were identified by time-of-flight secondary ion mass spectrometric analysis of the collected spots, suggesting that the particles originated from the lower airways. The amount of particles collected in ten exhalations was sufficient for characterizing the phospholipid composition. The feasibility of the technique in respiratory research is demonstrated by analysis of the phospholipid composition of exhaled particles from healthy controls, patients with asthma, and patients with cystic fibrosis. We believe this technology will be useful for monitoring patients with respiratory disease and has a high potential to detect new biomarkers in exhaled air.
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| 2. |
- Bellner, Lars, 1973, et al.
(author)
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A proinflammatory peptide from herpes simplex virus type 2 glycoprotein G affects neutrophil, monocyte, and NK cell functions
- 2005
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In: J Immunol. - : American Association of Immunologists. ; 174:4, s. 2235-2241
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Journal article (peer-reviewed)abstract
- We have identified a synthetic peptide derived from the secreted portion of HSV type 2 glycoprotein G, denoted gG-2p20, which has proinflammatory properties in vitro. The gG-2p20 peptide, corresponding to aa 190-205 of glycoprotein G-2, was a chemoattractant for both monocytes and neutrophils in a dose-dependent fashion, and also induced the release of reactive oxygen from these cells. The receptor mediating the responses was identified as the formyl peptide receptor. The gG-2p20-induced activation of phagocytes had a profound impact on NK cell functions. The reactive oxygen species produced by gG-2p20-activated phagocytes both inhibited NK cell cytotoxicity and accelerated the apoptotic cell death in NK cell-enriched lymphocyte populations. Hence, we have for the first time been able to identify a potential function of the secreted portion of HSV-2 glycoprotein G. We propose that the proinflammatory gG-2p20 peptide identified could contribute to a reduced function and viability of NK cells during HSV-2 infection due to its ability to recruit and activate phagocytic cells.
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| 3. |
- Berglin, Mattias, 1970, et al.
(author)
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Fibrinogen Adsorption and Conformational Change on Model Polymers: Novel Aspects of Mutual Molecular Rearrangement
- 2009
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 25:10, s. 5602-5608
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Journal article (peer-reviewed)abstract
- By combining quartz crystal microbalance with dissipation monitoring (QCM-D) and surface plasmon resonance (SPR), the organic mass, water content, and corresponding protein film structure of fibrinogen adsorbed to acrylic polymeric substrates with varying polymer chain flexibility was investigated. Albumin and immunoglobulin G were included as reference proteins. For fibrinogen, the QCM-D model resulted in decreased adsorbed mass with increased polymer chain flexibility. This stands in contrast to the SPR model, in which the adsorbed mass increased with increased polymer chain flexibility. As the QCM-D model includes the hydrodynamically coupled water, we propose that on the nonflexible polymer significant protein conformational change with water incorporation in the protein film takes place. Fibrinogen maintained a more native conformation on the flexible polymer, probably due to polymer chain rearrangement rather than protein conformational change. In comparison with immunoglobulin G and albumin, polymer chain flexibility had only minor impact on adsorbed mass and protein structure. Understanding the adsorption and corresponding conformational change of a protein together with the mutual rearrangement of the polymer chain upon adsorption not only has implications in biomaterial science but could also increase the efficacy of molecular imprinted polymers (MIPs).
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| 4. |
- Bergquist, Magnus, 1960, et al.
(author)
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Practising Peer Review in Organizations: a Qualifier for Knowledge Dissemination and Legitimization
- 2001
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In: Journal of Information Technology. - London : SAGE Publications. ; 16:2, s. 99-112
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Journal article (peer-reviewed)abstract
- A key issue in many organizations is how to disseminate information in an effective way and, more importantly, how to make use of this information in order to create new knowledge. One way of addressing this problem is to focus on how information is socially transformed into knowledge. This includes how knowledge is handled in practice and how the knowledge produced is qualiŽ ed as being something worth knowing and acting upon. Two well-established practices for doing this are the refereeing system and the peer review process. These are used in scientiŽ c communities as a means of validating and legitimating knowledge, for example by reviewing journal papers before publishing or project proposals before granting funds, etc. This paper argues that peer review is a useful concept when looking at knowledge creation and legitimization in organizations. The social meaning of peer review is to legitimize new knowledge by organizationally sanctioning it and thereby creating a platform for collective sense making. This paper uses an example from a Ž eld study in a pharmaceutical company in order to illustrate this argument. The study took place in a quality support department where the quality of health care products and processes was assessed. The organization had a need for fast and reliable updating of information that could in uence how the production process of pharmaceuticals should be carried out. In order to cope with these problems the department established an ‘evaluation loop’, which shared several characteristics with the peer review process.
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| 5. |
- Bergquist, Magnus, 1960, et al.
(author)
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The Power of Gifts: Organizing Social Relationships in Open Source Communities
- 2001
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In: Information Systems Journal. - Chichester : Wiley. ; 11:4, s. 305-320
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Journal article (peer-reviewed)abstract
- In writings on the open source software development model, it is often argued that it is successful as a result of the gift economy that embraces activities in online communities. However, the theoretical foundations for this argument are seldom discussed and empirically tested. Starting with the ‘classic’ theories of gift giving, we discuss how they need to be developed in order to explain giftgiving practices in digital domains. In this paper, we argue that the gift economy is important, not only because it creates openness, but also because it organizes relationships between people in a certain way. Open source software development relies on gift giving as a way of getting new ideas and prototypes out into circulation. This also implies that the giver gets power from giving away. This power is used as a way of guaranteeing the quality of the code. We relate this practice to how gifts, in the form of new scientific knowledge, are given to the research community, and how this is done through peer review processes.
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| 6. |
- Bergstrand, Fredrik, 1983, et al.
(author)
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Using live video for information sharing in emergency response work
- 2009
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In: International Journal of Emergency Management. - 1471-4825 .- 1741-5071. ; 6:3/4, s. 295-301
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Journal article (peer-reviewed)abstract
- This paper presents findings from a design-oriented study focusing on emergency response work. Traditionally, Information Technology (IT) for emergency response work has included en route navigation advice, resource management, hazard material databases, property information repositories and situation reporting using sketching functionality. Now, a new class of IT has become available, namely mobile live video capabilities. This paper presents initial findings from a study on how mobile live video capabilities can improve information sharing and situation awareness in emergency response work.
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| 7. |
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| 8. |
- Dittmer, Staffan, 1970, et al.
(author)
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In situ Raman studies of single-walled carbon nanotubes grown by local
- 2008
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In: Chemical Physics Letters. - : Elsevier BV. - 0009-2614 .- 1873-4448. ; 457
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Journal article (peer-reviewed)abstract
- Using in situ Raman spectroscopy we investigate single wall carbon nanotube growth on Mo electrodes, using a highly localized resistive heating technique. Small diameter semiconducting single wall nanotubes grow very rapidly when the catalyst support is heated to a temperature of 800 C. The G/D ratio shows an interesting time-dependent behaviour. It first decreases, indicating the presence of amorphous carbon and then significantly increases again after ca. 5 min growth while retaining the position and shape expected for predominantly semiconducting carbon nanotubes.
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| 9. |
- George-Chandy, Annie, 1969, et al.
(author)
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Th17 development and autoimmune arthritis in the absence of reactive oxygen species
- 2008
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In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 38:4, s. 1118-1126
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Journal article (peer-reviewed)abstract
- Dendritic cells (DC) express a functional NADPH oxidase and produce reactive oxygen species (ROS) upon interaction with microbes and T cells. Exposure to ROS leads to DC activation and maturation, as evidenced by phenotypic and functional changes. We have evaluated how endogenous ROS production affects the cytokine secretion pattern and T cell-activating capacity of bone marrow-derived murine DC. DC treated with ROS scavengers, as well as DC from mice that lack a functional NADPH oxidase (and thereby inherently deficient in ROS production) produced significantly increased levels of IL-1β, IL-6, TNF-α and TGF-β in response to microbial activation. DC deficient in ROS production induced high levels of IFN-γ and IL-17 in responding T cells after Ag-specific or superantigen-induced activation. Finally, we show that ROS deficiency affected the induction of a T cell-dependent inflammatory condition, collagen-induced arthritis (CIA). C57BL/6 mice that lack a functional NADPH oxidase developed a severe and erosive CD4-dependent CIA, whereas the majority of the congenic wild-type animals remained healthy. These data suggest that ROS act as immunomodulators in DC-driven T cell activation and perhaps also in T cell-dependent immunopathology.
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| 10. |
- Guillot, Gilles, 1972, et al.
(author)
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Spatial prediction of weed intensities from exact count data and image-based estimates
- 2009
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In: Journal of the Royal Statistical Society Series C-Applied Statistics. - : Oxford University Press (OUP). - 0035-9254 .- 1467-9876. ; 58, s. 525-542
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Journal article (peer-reviewed)abstract
- Collecting weed exact counts in an agricultural field is easy but extremely time consuming. Image analysis algorithms for object extraction applied to pictures of agricultural fields may be used to estimate the weed content with a high resolution (about 1 m(2)), and pictures that are acquired at a large number of sites can be used to obtain maps of weed content over a whole field at a reasonably low cost. However, these image-based estimates are not perfect and acquiring exact weed counts also is highly useful both for assessing the accuracy of the image-based algorithms and for improving the estimates by use of the combined data. We propose and compare various models for image index and exact weed count and we use them to assess how such data should be combined to obtain reliable maps. The method is applied to a real data set from a 30-ha field. We show that using image estimates in addition to exact counts allows us to improve the accuracy of maps significantly. We also show that the relative performances of the methods depend on the size of the data set and on the specific methodology (full Bayes versus plug-in) that is implemented.
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