| 41. |
- Del Barga, I., et al.
(författare)
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Effects of algal extracts (Polysiphonia fucoides) on rainbow trout (Oncorhynchus mykiss): A biomarker approach
- 2006
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Ingår i: Marine Environmental Research. - : Elsevier BV. - 0141-1136. ; 62:Supplement 1, s. S283-S286
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Tidskriftsartikel (refereegranskat)abstract
- The genotoxicity of algal extracts (Polysiphonia fucoides) was investigated in erythrocytes of rainbow trout (Oncorhynchus mykiss). Trout were exposed to 0.5% of the algal extract for 7 days. Comet assay (alkaline and neutral versions) and Micronucleus test were used to assess DNA damage, and Diffusion Assay to detect apoptotic cells. EROD activities and oxidative stress parameters in rainbow trout liver were also measured. A significant induction of DNA single strand breaks comparable to the ones induced by the in vivo exposure to 20 mg/kg B[a]P was observed at the end of the treatment, while increases of double strand breaks and apoptotic cells were not observed. The absence of activation of antioxidant responses seems to underline a mechanism of action of the genotoxic algal extract which does not involve oxidative stress.
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| 42. |
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| 43. |
- Ek, Helene, 1976, et al.
(författare)
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Tentative biomarkers for 2,4,6-trinitrotoluene (TNT) in fish (Oncorhynchus mykiss)
- 2005
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Ingår i: Aquatic Toxicology. - : Elsevier BV. - 0166-445X .- 1879-1514. ; 72:3, s. 221-230
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Tidskriftsartikel (refereegranskat)abstract
- 2,4,6-Trinitrotoluene (TNT) is the major explosive in ammunition dumped into lakes and the sea after World War II. To identify useful biomarkers of TNT-exposure for forthcoming fish monitoring studies at ammunition dumping sites, rainbow trout (Oncorhynchus mykiss) were intraperitoneal (i.p.) injected with TNT in peanut oil at doses of 0, 100, 200 or 400 mg TNT/kg body weight and sampled 72 h later. The study covered blood parameters, and hepatic antioxidant and detoxifying enzymes. Fish treated with TNT had an increased glutathione S-transferase (GST) activity and glutathione reductase (GR) activity, and a decreased percentage of oxidised glutathione (%GSSG) compared to the control group. In addition to increased methemoglobin, the increased glutathione and glutathione dependent enzyme activities indicate that TNT oxidises macromolecules and activates antioxidant defence systems which may be useful as general biomarkers of TNT-exposure. The fish bile was analysed for TNT and its metabolites by gas chromatography-mass spectrometry (GC-MS), and the toxicity of the bile was determined with the cladoceran Ceriodaphnia dubia. A dose-dependent increase in TNT, 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) was found in the hydrolysed bile of the TNT-treated fish. These results indicate that the fish are able to detoxify and excrete TNT and suggest that the detection of TNT, 2-ADNT and 4-ADNT in bile may be suitable as a direct marker of exposure to TNT. (c) 2005 Elsevier B.V. All rights reserved.
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| 44. |
- Frenzilli, G., et al.
(författare)
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DNA damage in eelpout (Zoarces viviparus) from Göteborg harbour
- 2004
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Ingår i: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. - : Elsevier BV. - 0027-5107. ; 552:1-2, s. 187-195
-
Tidskriftsartikel (refereegranskat)abstract
- The relationship between DNA damage and the exposure of marine organisms to environmental contaminants was examined in the Göteborg harbour area. This research is part of a wider ecotoxicological study planned to evaluate the biological impact of chemical contamination in the River Göta estuary, following a bunker oil (10–100 tonnes) spill occurred in June 2003. Here we present data on the DNA strand breaks derived using the comet assay and the presence of apoptotic cells using the diffusion assay in nucleated erythrocytes of the eelpout (Zoarces viviparus) from the study area and at a clean reference site. Polycyclic aromatic hydrocarbon metabolites were also analyzed in the bile of exposed fish. The results showed a high level of damaged DNA, paralleled by a peak in bile PAH metabolites, in fish from the most impacted site, 3 weeks after the oil spill. A significant recovery was observed in specimens from the spill site, 5 months later, but not in fish caught in the middle part of Göteborg harbour, which is chronically subjected to heavy chemical pollution. The levels of apoptic cells did not show any marked variations, but a significant recovery was observed in fish from the oil impacted site 5 months after the spill
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| 45. |
- Förlin, Lars, 1950, et al.
(författare)
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A tiered test system for fish toxicity
- 2004
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Ingår i: Better chemicals control within Reach. - 9172837047 ; , s. 109-117
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 46. |
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| 47. |
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| 48. |
- Förlin, Lars, 1950, et al.
(författare)
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Epidemiological studies indicate a cause-effect relationship between pulp mill effluent exposure and male-biased sex ratios in fish.
- 2004
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Ingår i: Platform presentation at the 5th International Conference on Fate and Effects of Pulp and Paper mill Effluents. Seattle, Washington, June 1-4, 2003. Extended abstract published in: Pulp and Paper Mill Effluent Environmental Fate and Effects Conference Proceedings. Ed. D. Borton, T.J. Hall, R.P. Fischer and J.F. Fischer. DEStech Publications, Inc., Lancaster. - Lancaster : DEStech Publ, Inc. ; , s. 48-54, s. 48-54
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Konferensbidrag (refereegranskat)
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| 49. |
- Förlin, Lars, 1950, et al.
(författare)
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Health status in fish in eight polluted coastal sites in Sweden.
- 2022
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Ingår i: Conference: Pollutant Responses In Marine Organisms (PRIMO21) in May 22-25, 2022. Poster paper abstract.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The health of perch and eelpout were assessed by effect-based methods (including physiological and biochemical endpoints, i.e. biomarkers) in eight different polluted hot spot areas along the Swedish coast. An important purpose was to get an overview of the severity of impacts of environmental pollutants in the polluted areas compared to the reference sites. Taken together, the results suggest moderate to severe impacts on the fish health in all hot spot areas studied. The results indicate that coastal areas act as sources of environmentally harmful substances to the aquatic environment. In addition, the results contributes to the assessment of biological effects of contaminants, which is an important component within EUs Marine Strategy Framework Directive.
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| 50. |
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