| 151. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for Laboratory Evaluation and Analytical Performance Characteristics reporting of clinical measurement procedures
- 2023
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Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation. - : TAYLOR & FRANCIS LTD. - 0036-5513 .- 1502-7686. ; 83:7, s. 467-469
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript.The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 152. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for Laboratory Evaluation and Analytical Performance characteristics reporting of clinical measurement procedures
- 2023
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Ingår i: Clinica Chimica Acta. - : ELSEVIER. - 0009-8981 .- 1873-3492. ; 551
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peerreviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 153. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP Checklist for Laboratory Evaluation and Analytical Performance Characteristics Reporting of Clinical Measurement Procedures
- 2024
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Ingår i: ANNALS OF LABORATORY MEDICINE. - : KOREAN SOC LABORATORY MEDICINE. - 2234-3806. ; 44:2, s. 122-125
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practi-tioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describ-ing the analytical performance of measurement procedures. These variations also chal-lenge authors, readers, reviewers, and editors in deciding the quality of a submitted manu-script. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recom-mends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the Laboratory Evaluation and Analytical Performance Char-acteristics (LEAP) checklist will improve the standardisation of journal publications describ-ing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 154. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures
- 2023
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Ingår i: Biochemia Medica. - : CROATIAN SOC MEDICAL BIOCHEMISTRY & LABORATORY MEDICINE. - 1330-0962 .- 1846-7482. ; 33:3
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Forskningsöversikt (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the Laboratory Evaluation and Analytical Performance Characteristics (LEAP) checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 155. |
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| 156. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures
- 2024
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Ingår i: PRACTICAL LABORATORY MEDICINE. - : ELSEVIER. - 2352-5517. ; 39
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 157. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures
- 2023
-
Ingår i: Clinical Chemistry and Laboratory Medicine. - : WALTER DE GRUYTER GMBH. - 1434-6621 .- 1437-4331.
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 158. |
- Loh, Tze Ping, et al.
(författare)
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The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures
- 2024
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Ingår i: Annals of Clinical Biochemistry. - : Sage Publications. - 0004-5632 .- 1758-1001. ; 61:1, s. 3-7
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Tidskriftsartikel (refereegranskat)abstract
- Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.
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| 159. |
- Lonndahl, Louise, et al.
(författare)
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Decreased innervation of eczematous skin in NC/Nga atopic mice during chronic mild stress
- 2010
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Ingår i: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY. - : Informa UK Limited. - 0892-3973 .- 1532-2513. ; 32:1, s. 147-152
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Tidskriftsartikel (refereegranskat)abstract
- Material and methods: We used three groups of mice, stressed control (SC, stressed but not immunized with a mite antigen), non-stressed eczematous (NSE, not stressed but immunized) and stressed eczematous (SE, stressed and immunized). Results: There was a decrease of protein gene product (PGP) 9.5 positive nerve fibers in SE compared to SC mice, in both epidermis and dermis, also in SE compared to NSE mice. Also a decrease in growth associated protein (GAP)-43 positive nerve fibers was seen in SE compared to SC, in both epidermis and dermis. Conclusion: Chronic mild stress in atopic mice leads to decreased innervation in eczematous lesions.
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| 160. |
- Lund, Mari L., et al.
(författare)
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Enterochromaffin 5-HT cells : A major target for GLP-1 and gut microbial metabolites
- 2018
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Ingår i: MOLECULAR METABOLISM. - : Elsevier. - 2212-8778. ; 11, s. 70-83
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Tidskriftsartikel (refereegranskat)abstract
- Objectives5-HT storing enterochromaffin (EC) cells are believed to respond to nutrient and gut microbial components, and 5-HT receptor-expressing afferent vagal neurons have been described to be the major sensors of nutrients in the GI-tract. However, the molecular mechanism through which EC cells sense nutrients and gut microbiota is still unclear.Methods and resultsTPH1, the 5-HT generating enzyme, and chromogranin A, an acidic protein responsible for secretory granule storage of 5-HT, were highly enriched in FACS-purified EC cells from both small intestine and colon using a 5-HT antibody-based method. Surprisingly, EC cells from the small intestine did not express GPCRsensors for lipid and protein metabolites, such as FFAR1, GPR119, GPBAR1(TGR5), CaSR, and GPR142, in contrast to the neighboring GLP-1 storing enteroendocrine cell. However, the GLP-1 receptor was particularly highly expressed and enriched in EC cells as judged both by qPCR and by immunohistochemistryusing a receptor antibody. GLP-1 receptor agonists robustly stimulated 5-HT secretion from intestinal preparations using both HPLC and a specific amperometricmethod. Colonic EC cells expressed many different types of known and potential GPCR sensors of microbial metabolites including three receptors for SCFAs, i.e. FFAR2, OLF78, and OLF558 and receptors for aromatic acids, GPR35; secondary bile acids GPBAR1; and acyl-amides and lactate, GPR132.ConclusionNutrient metabolites apparently do not stimulate EC cells of the small intestine directly but through a paracrine mechanism involving GLP-1 secreted from neighboring enteroendocrine cells. In contrast, colonic EC cells are able to sense a multitude of different metabolites generated by the gut microbiota as well as gut hormones, including GLP-1.
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