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| 2. |
- Barnoin, Guillaume, et al.
(författare)
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Intermolecular dark resonance energy transfer (DRET): Upgrading fluorogenic DNA sensing
- 2021
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Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 49:12
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Tidskriftsartikel (refereegranskat)abstract
- The sensitivity of FRET-based sensing is usually limited by the spectral overlaps of the FRET donor and acceptor, which generate a poor signal-to-noise ratio. To overcome this limitation, a quenched donor presenting a large Stokes shift can be combined with a bright acceptor to perform Dark Resonance Energy Transfer (DRET). The consequent fluorogenic response from the acceptor considerably improves the signal-to-noise ratio. To date, DRET has mainly relied on a donor that is covalently bound to the acceptor. In this context, our aim was to develop the first intermolecular DRET pair for specific sensing of nucleic acid sequences. To this end, we designed DFK, a push-pull probe based on a fluorenyl π-platform that is strongly quenched in water. DFK was incorporated into a series of oligonucleotides and used as a DRET donor with Cy5-labeled complementary sequences. In line with our expectations, excitation of the dark donor in the double-labeled duplex switched on the far-red Cy5 emission and remained free of cross-excitation. The DRET mechanism was supported by time-resolved fluorescence measurements. This concept was then applied with binary probes, which confirmed the distance dependence of DRET as well as its potency in detecting sequences of interest with low background noise.
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| 3. |
- Baste, Valborg, et al.
(författare)
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Radiofrequency exposure on fast patrol boats in the Royal Norwegian Navy-an approach to a dose assessment.
- 2010
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Ingår i: Bioelectromagnetics. - : Wiley. - 0197-8462 .- 1521-186X. ; 31:5, s. 350-360
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Tidskriftsartikel (refereegranskat)abstract
- Epidemiological studies related to radiofrequency (RF) electromagnetic fields (EMF) have mainly used crude proxies for exposure, such as job titles, distance to, or use of different equipment emitting RF EMF. The Royal Norwegian Navy (RNoN) has measured RF field emitted from high-frequency antennas and radars on several spots where the crew would most likely be located aboard fast patrol boats (FPB). These boats are small, with short distance between the crew and the equipment emitting RF field. We have described the measured RF exposure aboard FPB and suggested different methods for calculations of total exposure and annual dose. Linear and spatial average in addition to percentage of ICNIRP and squared deviation of ICNIRP has been used. The methods will form the basis of a job exposure matrix where relative differences in exposure between groups of crew members can be used in further epidemiological studies of reproductive health. Bioelectromagnetics, 2010. (c) 2010 Wiley-Liss, Inc.
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| 4. |
- Björck-Åkesson, Eva, 1952-, et al.
(författare)
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ICF och ICF-CY - Historik och utveckling
- 2011. - 1:1
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Ingår i: Handbok i att använda ICF och ICF-CY. - Lund : Studentlitteratur. - 9789144056371 ; , s. 71-90
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 5. |
- Blomberg, Git
(författare)
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En likvärdig förskola – vad är det?
- 2023
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Ingår i: Förskoleforum.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Förskolans utbildning ska vara likvärdig oavsett var i landet den anordnas. Men hur ser det egentligen ut med likvärdigheten? Barn får olika tillgång till en kvalitativ utbildning och till specialpedagogisk kompetens beroende på var i landet de bor.
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| 6. |
- Ernberg, M., et al.
(författare)
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Experimental myalgia induced by repeated infusion of acidicsaline into the human masseter muscle does not cause the release of algesic substances
- 2013
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Ingår i: European Journal of Pain. - : John Wiley & Sons. - 1090-3801 .- 1532-2149. ; 17:4, s. 539-550
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND:Animal studies have shown that two repeated intramuscular injections of acidic saline induce mechanical allodynia that lasts for 4 weeks with spread to the contralateral side. In this study, we tested the hypothesis that two repeated intramuscular infusions of acidic saline into the human masseter muscle is associated with pain, mechanical allodynia and release of algesic substances. Eighteen healthy volunteers participated. On day 1, 2.5 mL of acidic saline (pH 3.3) was infused into one of the masseter muscles and isotonic saline (pH 6.0) into the other (randomized and single-blind). Two days later, intramuscular microdialysis was performed to sample serotonin, glutamate, pyruvate, lactate and glucose, during which the saline infusions were repeated. Pain and pressure pain thresholds (PPTs) were recorded before and after infusions on both days.RESULTS:Pain intensity induced by the infusions was higher after acidic than that after isotonic saline (p < 0.05). PPTs were decreased on both sides after microdialysis compared with baseline day 1 (p's < 0.05), but there were no differences in PPTs between sides at any time point. The levels of serotonin, glutamate, pyruvate, lactate or glucose did not change significantly during microdialysis.CONCLUSION:Infusion of acidic saline caused low levels of muscle pain, but no mechanical allodynia and no increased release of algesic substances. The value of this model appears modest, but future studies could be performed with larger sample size and higher flow rate before definite conclusions about the validity of the model for craniofacial myalgia can be drawn.
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| 9. |
- Holmström, Barry
(författare)
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Sweden
- 1995
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Ingår i: The Global Expansion of Judicial Power. - : New York:New York University Press. - 0814782094
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Bokkapitel (refereegranskat)
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| 10. |
- Idström, Alexander, 1983, et al.
(författare)
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13C NMR assignments of regenerated cellulose from solid-state 2D NMR spectroscopy
- 2016
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Ingår i: Carbohydrate Polymers. - : Elsevier BV. - 0144-8617. ; 151, s. 480-487
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Tidskriftsartikel (refereegranskat)abstract
- From the assignment of the solid-state 13C NMR signals in the C4 region, distinct types of crystalline cellulose, cellulose at crystalline surfaces, and disordered cellulose can be identified and quantified. For regenerated cellulose, complete 13C assignments of the other carbon regions have not previously been attainable, due to signal overlap. In this study, two-dimensional (2D) NMR correlation methods were used to resolve and assign 13C signals for all carbon atoms in regenerated cellulose. 13C-enriched bacterial nanocellulose was biosynthesized, dissolved, and coagulated as highly crystalline cellulose II. Specifically, four distinct 13C signals were observed corresponding to conformationally different anhydroglucose units: two signals assigned to crystalline moieties and two signals assigned to non-crystalline species. The C1, C4 and C6 regions for cellulose II were fully examined by global spectral deconvolution, which yielded qualitative trends of the relative populations of the different cellulose moieties, as a function of wetting and drying treatments.
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