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| 43. |
- Abbasi Aval, Negar, 1986-
(författare)
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Utilizing Biopolymers in 3D Tumor Modeling and Tumor Diagnosis
- 2023
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Cancer represents a significant global public health challenge and ranks as the second mostcommon cause of death in the United States. The onset of cancer entails an initial phasewhere cells lose their polarity and disconnect from the normal basement membrane, allowingthem to form distinct three-dimensional (3D) configurations that interact with adjacent cellsand the surrounding microenvironment. Cells grown in 2D monolayers demonstrate differentgene expression patterns and different activation of signaling pathways compared to cellscultivated within the natural structure of tumor tissue of the same cell type. Multicellulartumor spheroids (MCTS) are extensively investigated as a well-studied model of organotypiccancer. These spheroids are formed by tumor cells, either alone or in combination with othercell types, and they can be created with or without the application of supportive scaffolds.The MCTSs are also considered promising models for preclinical assessments of chemosensitivity.However, the creation of these tumor spheroids presents challenges, as not alltumor cell lines can consistently form regular spheroids.Cellulose nanofibrils (CNF) have become essential as a sustainable and environmentallyfriendly material. For example, thin films, with inherent mechanical properties, and flexibility,offer versatility across various applications. Also known for its biocompatibility and non-toxicnature, native CNF is a natural option to use. Its fibrous structure closely mimics the collagenmatrix in human tissue, showing potential as an effective scaffold for 3D cell culture. In thisregard, an innovative Layer-by-Layer (LbL) coating technique using CNF-polyelectrolytebilayers was investigated to generate spheroids. This method constructs bilayers of CNFand polyelectrolytes and can coat various surfaces. In this thesis, the first focus was ondemonstrating the spheroid formation capability using low molecular weight polyelectrolytesin LbL assembly. Secondly, an investigation was conducted involving embedding of LbLgrownspheroids in a decellularized extracellular matrix (ECM) aiming to determine howECM, possessing suitable mechanical characteristics, could influence the cancer stem celltraits in spheroids. Thirdly, the thesis demonstrated the utilization of LbL for capturing andreleasing of circulating tumor cells. Lastly, the shift from using low molecular weightpolyelectrolytes in the LbL assembly to high molecular weight counterparts and analyzingthe differences in spheroid formation abilities to assess the underlying differences inmolecular weights of the polyelectrolytes was explored. All-in-all, employing the CNF-basedLbL surface coating strategy explored in the thesis has proven to be promising for thedevelopment of spheroid models closely resembling in vivo conditions and holds significantpotential for applications in drug development.
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| 45. |
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| 46. |
- Abbasi, Saeed
(författare)
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Characterisation of airborne particles from rail traffic
- 2011
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Since the investigation of wear particles in rail transport started in late-1910s, the high mass concentration of these particles has raised worries among researchers concerned with air quality. However, effective action has yet to be taken because of lack of relevant knowledge. This thesis provides applicable information for the airborne wear particles in rail transport. Some aspects of their characteristics such as diameter size, mass concentration, number concentration, and morphology of particles were investigated in field tests and laboratory tests.The effects on particle characterisations from different operational conditions in the field tests, and applying different braking materials, conducting tests in different applied loads or sliding velocities in the laboratory tests were studied. The main advantage of conducting laboratory tests was to focus on studying particles from one source. The possibility of repetition, using high sensitive instruments and conducting tests at low costs are the other advantages of laboratory studies. Paper A describes how a pin-on-disc machine was used to reproduce similar real operational conditions during mechanical braking in a train. The results were validated by comparing the field tests results with the laboratory studies. The particles morphology and size distribution were also studied.Paper B presents a summary of field tests results. The effects of curve negotiating and applying braking in different real conditions were investigated with an on-board measurement.The element composition of the particles and their potential sources were also investigated outside of the particles morphologies.Paper C presents comprehensive results from laboratory studies on airborne particles from different braking materials. The differences in the particle characteristics in similar test conditions were attributable to different material compositions and dominant wear mechanisms. A new index was introduced in this paper and is suggested to be used as a qualitative factor with regard to the airborne wear particle emission rate.Paper D is a review of the recent studies of exhaust emission and non-exhaust emission from rail vehicles. A summary of results, measurements, adverse health effects, and proposed or applied solutions are reviewed in this paper.
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| 47. |
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| 48. |
- Abbasi, Saeed, 1973-
(författare)
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Towards elimination of airborne particles from rail traffic
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Since the investigation of wear particles from rail transport started in the late 1910s, the high mass concentrations of these particles have prompted concern among researchers interested in air quality. However, effective action has yet to be taken because relevant knowledge is still missing. This thesis provides knowledge of airborne wear particles originating from rail transport. Some aspects of their characteristic parameters, such as size, mass concentration, number concentration, and morphology, were investigated in the field and in laboratory tests. We also discuss means to mitigate non-exhaust emissions, as well as the advantages and disadvantages of various test set-ups in the seven appended journal papers:Paper A reviews recent studies of exhaust and non-exhaust emissions from rail vehicles. The results, measurements, adverse health effects, and proposed or applied solutions presented in this literature are summarized in this paper.Paper B summarizes the results of field tests we conducted. The effects of curve negotiation and braking under different real conditions were investigated in a field test in which on-board measurements were made. The elemental composition and morphology of the particles emitted and their potential sources were also investigated.Paper C describes how a pin-on-disc machine can be used to reproduce real operating conditions during mechanical train braking in a controlled laboratory setting. The results were validated by comparing the field test results with the results of laboratory studies.Paper D presents comprehensive results of laboratory studies of airborne particles from different braking materials. A new index is introduced in this paper, which can be used as a quantitative metric for assessing airborne wear particle emission rates.Paper E describes the effects of using various friction modifiers and lubricants on the characteristics of airborne particles from wheel–rail contact under lubricated and unlubricated conditions.Paper F reports work to simulate thermoelastic instability in the cast-iron braking material. We simulated the fluctuation of the flash temperature by considering the temperature dependency of the material properties and the transformation of the contact state due to thermomechanical phenomena and wear.Paper G reviews new full- and sub-scale measurements of non-exhaust emissions from ground transport. The advantages and disadvantages of on-board measurements, pin-on-disc tests, dynamometer tests, and test rig studies are discussed in this paper.
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| 49. |
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| 50. |
- Abbasiverki, Roghayeh
(författare)
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Analysis of underground concrete pipelines subjected to seismic high-frequency loads
- 2016
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Buried pipelines are tubular structures that are used for transportation of important liquid materials and gas in order to provide safety for human life. During an earthquake, imposed loads from soil deformations on concrete pipelines may cause severe damages, possibly causing disturbance in vital systems, such as cooling of nuclear power facilities. The high level of safety has caused a demand for reliable seismic analyses, also for structures built in the regions that have not traditionally been considered as highly seismically active. The focus in this study is on areas with seismic and geological conditions corresponding to those in Sweden and Northern Europe. Earthquakes in Sweden for regions with hard rock dominated by high-frequency ground vibrations, Propagation of such high-frequency waves through the rock mass and soil medium affect underground structures such as pipelines.The aim of this project is investigating parameters that affect response of buried pipelines due to high-frequency seismic excitations. The main focus of the study is on reinforced concrete pipelines. Steel pipelines are also studied for comparison purposes. The effects of water mass, burial depth, soil layer thickness and non-uniform ground thickness caused by inclined bedrock are studied. The results are compared to those obtained for low-frequency earthquakes and the relationship between strong ground motion parameters and pipelines response is investigated. It is shown that, especially for high frequency earthquake excitations, non-uniform ground thickness due to inclined bedrock significantly increase stresses in the pipelines. For the conditions studied, it is clear that high-frequency seismic excitation is less likely to cause damage to buried concrete pipelines. However, the main conclusion is that seismic analysis is motivated also for pipelines in high-frequency earthquake areas since local variation in the ground conditions can have a significant effect on the safety.
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