| 261. |
|
|
| 262. |
- Brown, GR, et al.
(författare)
-
Parallel changes in nuclear and cytosolic calcium in mouse pancreatic beta-cells
- 1997
-
Ingår i: The Biochemical journal. - : Portland Press Ltd.. - 0264-6021 .- 1470-8728. ; 325325 ( Pt 3), s. 771-778
-
Tidskriftsartikel (refereegranskat)abstract
- In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+]c) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+]c. This implies that any signal involved in increasing [Ca2+]c, and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
|
|
| 263. |
- Bychkov, A.M., et al.
(författare)
-
Spin-dependent electron behaviour in quantum point contacts
- 2000
-
Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 11:4, s. 318-322
-
Tidskriftsartikel (refereegranskat)abstract
- The effect of spontaneous spin polarization in quantum point contacts (QPCs) is investigated by self-consistent modelling within the Kohn-Sham local spin-density formalism. The existence of the spin-polarized state of the QPC channel in the low-density regime is shown to change the value and the shape of the effective potential barrier for up- and down-spin electrons. We suggest that this effect can be relevant to the explanation of the anomalous 0.7-structure in the QPC conductance and may assist spin injection in potential spintronic devices.
|
|
| 264. |
|
|
| 265. |
- Cabotaje, Princess R., et al.
(författare)
-
Probing Substrate Transport Effects on Enzymatic Hydrogen Catalysis : An Alternative Proton Transfer Pathway in Putatively Sensory [FeFe] Hydrogenase
- 2023
-
Ingår i: ACS Catalysis. - : American Chemical Society (ACS). - 2155-5435. ; 13:15, s. 10435-10446
-
Tidskriftsartikel (refereegranskat)abstract
- [FeFe] hydrogenases, metalloenzymes catalyzing proton/dihydrogeninterconversion, have attracted intense attention due to their remarkablecatalytic properties and (bio-)technological potential for a futurehydrogen economy. In order to unravel the factors enabling their efficientcatalysis, both their unique organometallic cofactors and proteinstructural features, i.e., "outer-coordination sphere"effects have been intensively studied. These structurally diverseenzymes are divided into distinct phylogenetic groups, denoted asGroup A-D. Prototypical Group A hydrogenases display high turnoverrates (10(4)-10(5) s(-1)).Conversely, the sole characterized Group D representative, Thermoanaerobacter mathranii HydS (TamHydS), shows relatively low catalytic activity (specific activity10(-1) & mu;mol H-2 mg(-1) min(-1)) and has been proposed to serve a H-2-sensory function. The various groups of [FeFe] hydrogenaseshare the same catalytic cofactor, the H-cluster, and the structuralfactors causing the diverging reactivities of Group A and D remainto be elucidated. In the case of the highly active Group A enzymes,a well-defined proton transfer pathway (PTP) has been identified,which shuttles H+ between the enzyme surface and the activesite. In Group D hydrogenases, this conserved pathway is absent. Here,we report on the identification of highly conserved amino acid residuesin Group D hydrogenases that constitute a possible alternative PTP.We varied two proposed key amino acid residues of this pathway (E252and E289, TamHydS numbering) via site-directed mutagenesisand analyzed the resulting variants via biochemical and spectroscopicmethods. All variants displayed significantly decreased H-2-evolution and -oxidation activities. Additionally, the variantsshowed two redox states that were not characterized previously. Thesefindings provide initial evidence that these amino acid residues arecentral to the putative PTP of Group D [FeFe] hydrogenase. Since theidentified residues are highly conserved in Group D exclusively, ourresults support the notion that the PTP is not universal for differentphylogenetic groups in [FeFe] hydrogenases.
|
|
| 266. |
|
|
| 267. |
- Campos Pereira, H., et al.
(författare)
-
Sorption of perfluoroalkyl substances (PFASs) to an organic soil horizon – Effect of cation composition and pH
- 2018
-
Ingår i: Chemosphere. - : Elsevier. - 0045-6535 .- 1879-1298. ; 207, s. 183-191
-
Tidskriftsartikel (refereegranskat)abstract
- Accurate prediction of the sorption of perfluoroalkyl substances (PFASs) in soils is essential for environmental risk assessment. We investigated the effect of solution pH and calculated soil organic matter (SOM) net charge on the sorption of 14 PFASs onto an organic soil as a function of pH and added concentrations of Al3+, Ca2+ and Na+. Often, the organic C-normalized partitioning coefficients (KOC) showed a negative relationship to both pH (Δlog KOC/ΔpH = −0.32 ± 0.11 log units) and the SOM bulk net negative charge (Δlog KOC = −1.41 ± 0.40 per log unit molc g−1). Moreover, perfluorosulfonic acids (PFSAs) sorbed more strongly than perfluorocarboxylic acids (PFCAs) and the PFAS sorption increased with increasing perfluorocarbon chain length with 0.60 and 0.83 log KOC units per CF2 moiety for C3–C10 PFCAs and C4, C6, and C8 PFSAs, respectively. The effects of cation treatment and SOM bulk net charge were evident for many PFASs with low to moderate sorption (C5–C8 PFCAs and C6 PFSA). However for the most strongly sorbing and most long-chained PFASs (C9–C11 and C13 PFCAs, C8 PFSA and perfluorooctane sulfonamide (FOSA)), smaller effects of cations were seen, and instead sorption was more strongly related to the pH value. This suggests that the most long-chained PFASs, similar to other hydrophobic organic compounds, are preferentially sorbed to the highly condensed domains of the humin fraction, while shorter-chained PFASs are bound to a larger extent to humic and fulvic acid, where cation effects are significant.
|
|
| 268. |
- Cederström, B., et al.
(författare)
-
Lesion characterization in spectral photon-counting tomosynthesis
- 2017
-
Ingår i: Medical Imaging 2017. - : SPIE - International Society for Optical Engineering. - 9781510607095
-
Konferensbidrag (refereegranskat)abstract
- It has previously been shown that 2D spectral mammography can be used to discriminate between (likely benign) cystic and (potentially malignant) solid lesions in order to reduce unnecessary recalls in mammography. One limitation of the technique is, however, that the composition of overlapping tissue needs to be interpolated from a region surrounding the lesion. The purpose of this investigation was to demonstrate that lesion characterization can be done with spectral tomosynthesis, and to investigate whether the 3D information available in tomosynthesis can reduce the uncertainty from the interpolation of surrounding tissue. A phantom experiment was designed to simulate a cyst and a tumor, where the tumor was overlaid with a structure that made it mimic a cyst. In 2D, the two targets appeared similar in composition, whereas spectral tomosynthesis revealed the exact compositional difference. However, the loss of discrimination signal due to spread from the plane of interest was of the same strength as the reduction of anatomical noise. Results from a preliminary investigation on clinical tomosynthesis images of solid lesions yielded results that were consistent with the phantom experiments, but were still to some extent inconclusive. We conclude that lesion characterization is feasible in spectral tomosynthesis, but more data, as well as refinement of the calibration and discrimination algorithms, are needed to draw final conclusions about the benefit compared to 2D.
|
|
| 269. |
- Chen, Miaoxiang, 1962-, et al.
(författare)
-
1 micron wavelength photo- and electroluminescence from a conjugated polymer
- 2004
-
Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 84:18, s. 3570-3572
-
Tidskriftsartikel (refereegranskat)abstract
- We report photo- and electroluminescence from an alternating conjugated polymer consisting of fluorene units and low-band gap donor-acceptor-donor (D-A-D) units. The D-A-D segment includes two electron-donating thiophene rings combined with a thiadiazolo-quinoxaline unit, which is electron withdrawing to its nature. The resulting polymer is conjugated and has a band gap of 1.27 eV. The corresponding electro- and photoluminescence spectra both peak at approximately 1 mum. Light-emitting diodes, based on a single layer of the polymer, demonstrated external quantum efficiencies from 0.03% to 0.05%.
|
|
| 270. |
|
|
