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Sökning: LAR1:gu > (2010) > Naturvetenskap

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Numrering	Referens	Omslagsbild	Hitta
1.	Olsson, Urban, 1954, et al. (författare) The Lanius excubitor (Aves, Passeriformes) conundrum – Taxonomic dilemma when molecular and non-molecular data tell different stories. 2010 Ingår i: Molecular Phylogenetics and Evolution. - : Elsevier BV. - 1055-7903 .- 1095-9513. ; 55:2, s. 347-357 Tidskriftsartikel (refereegranskat)abstract The phylogeny of 18 taxa in the Lanius excubitor complex, and the related species L. sphenocercus, L. ludovicianus and L. somalicus, was estimated based on the mitochondrial cytochrome b gene and the non-coding D-loop (in total ∼1.3 kb). According to the mitochondrial gene tree, Lanius excubitor s.l. is non-monophyletic, with some of its subspecies being more closely related to L. sphenocercus, L. ludovicianus, and L. somalicus. Also the division of the L. excubitor complex into a northern (L. excubitor) and a southern (L. meridionalis) species, as has been proposed based on morphological and ecological similarity and geographical distributions, is not compatible with the mitochondrial tree. Overall, genetic divergences among the ingroup taxa are small, indicating a recent radiation. A tree based on the nuclear ornithine decarboxylase (ODC) introns 6–7 is unresolved with respect to the ingroup, but provides strong support for a clade containing the Lanius excubitor complex, L. sphenocercus, L. ludovicianus and L. somalicus. We discuss the incongruence between the current taxonomy and the mitochondrial gene tree, and conclude that based on the latter the Lanius excubitor complex may be treated as at least six species, L. borealis, L. elegans, L. excubitor, L. lahtora, L. meridionalis, and L. uncinatus, but that other taxonomic treatments are also possible. However, uncertainty regarding to which extent the mitochondrial gene tree reflects the species phylogeny prevents us from recommending taxonomic change without further investigation. This study highlights the possible danger of relying on a single molecular marker, such as mitochondrial DNA, in taxonomic revisions and phylogenetic inference
2.	Franz, Jan, 1973, et al. (författare) Formation of carbon monoxide by radiative association: A quantum-mechanical study 2010 Ingår i: XVIII European Conference on Dynamics of Molecular Systems. Konferensbidrag (övrigt vetenskapligt/konstnärligt)
3.	Franz, Jan, 1973, et al. (författare) Molecule Formation by Radiative Association 2010 Ingår i: The Chemical Cosmos: Understanding Chemistry in Astronomical Environments. Konferensbidrag (övrigt vetenskapligt/konstnärligt)
4.	Franz, Jan, 1973, et al. (författare) Molecule Formation in Interstellar Space 2010 Ingår i: Department of Chemical- and Biological Engineering at Chalmers University of Technology and Chemistry at University of Gothenburg. Konferensbidrag (övrigt vetenskapligt/konstnärligt)
5.	Franz, Jan, 1973 (författare) On some molecular reactions of astrophysical interest 2010 Ingår i: Dynamics workshop by Lake Vänern. Konferensbidrag (övrigt vetenskapligt/konstnärligt)
6.	Janzon, Anders, 1978, et al. (författare) Exploring the microbial resistome in river sediments exposed to extraordinary high levels of antibiotics 2010 Ingår i: 35th FEBS Congress: Molecules of Life. Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract The rapid development and propagation of antibiotic resistance in pathogenic and opportunistic bacteria is a major threat to public health worldwide. The phenomenon has been widely studied in the clinical setting, but comparatively little is known about the prevalence and diversity of antibiotic resistance in communities of environmental bacteria, often referred to as the environmental resistome. As the external environment may function as a reservoir of resistance genes to human pathogens, we are interested in how environmental bacteria are affected by antibiotic pollution. We have previously isolated microbial DNA from river sediments taken up- and downstream from a water treatment plant that processes waste water from several pharmaceutical plants producing antibiotics. In a previous study, we used deep sequencing to identify unprecedented frequencies of known resistance genes to several classes of antibiotics in these samples. In this study, we aim to functionally characterize the resistome in a more open and exploratory way by screening genomic DNA libraries transformed into sensitive hosts. To generate the libraries, several experimental strategies were explored, including mechanical shearing and enzymatic digestion of the isolated DNA followed by blunt- or sticky end cloning into different plasmids, subsequently transformed into sensitive E. coli. Pros and cons of the different strategies will be discussed along with preliminary results of the screening against selected antibiotics.
7.	Reutgard, Martin, et al. (författare) Biologiska effekter - bedömningsgrunder under utveckling 2010 Ingår i: HAVET 2010 - Om miljötillståndet i svenska havsområdet. - 1654-6741. ; , s. 77-80 Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
8.	Franz, Jan, 1973 (författare) Some new theoretical tools for the study of electronically excited states of molecules 2010 Ingår i: Technical University Chemnitz. Konferensbidrag (övrigt vetenskapligt/konstnärligt)
9.	Abarenkov, Kessy, et al. (författare) The UNITE database for molecular identification of fungi – recent updates and future perspectives 2010 Ingår i: New Phytologist. - 0028-646X. ; 186:2, s. 281-285 Tidskriftsartikel (refereegranskat)
10.	Tedersoo, L., et al. (författare) 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases 2010 Ingår i: New Phytologist. - : Wiley. - 0028-646X. ; 188:1 Tidskriftsartikel (refereegranskat)abstract Compared with Sanger sequencing-based methods, pyrosequencing provides orders of magnitude more data on the diversity of organisms in their natural habitat, but its technological biases and relative accuracy remain poorly understood. This study compares the performance of pyrosequencing and traditional sequencing for species’ recovery of ectomycorrhizal fungi on root tips in a Cameroonian rain forest and addresses biases related to multi-template PCR and pyrosequencing analyses. Pyrosequencing and the traditional method yielded qualitatively similar results, but there were slight, but significant, differences that affected the taxonomic view of the fungal community. We found that most pyrosequencing singletons were artifactual and contained a strongly elevated proportion of insertions compared with natural intra- and interspecific variation. The alternative primers, DNA extraction methods and PCR replicates strongly influenced the richness and community composition as recovered by pyrosequencing. Pyrosequencing offers a powerful alternative for the identification of ectomycorrhizal fungi in pooled root samples, but requires careful selection of molecular tools. A well-populated backbone database facilitates the detection of biological and technical artifacts. The pyrosequencing pipeline is available at http://unite.ut.ee/454pipeline.tgz.

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