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- Clementi, Emily A., et al.
(författare)
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A Complex of Equine Lysozyme and Oleic Acid with Bactericidal Activity against Streptococcus pneumoniae
- 2013
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 8:11, s. Article Number: UNSP e80649-
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Tidskriftsartikel (refereegranskat)abstract
- HAMLET and ELOA are complexes consisting of oleic acid and two homologous, yet functionally different, proteins with cytotoxic activities against mammalian cells, with HAMLET showing higher tumor cells specificity, possibly due to the difference in propensity for oleic acid binding, as HAMLET binds 5-8 oleic acid molecules per protein molecule and ELOA binds 11-48 oleic acids. HAMLET has been shown to possess bactericidal activity against a number of bacterial species, particularly those with a respiratory tropism, with Streptococcus pneumoniae displaying the greatest degree of sensitivity. We show here that ELOA also displays bactericidal activity against pneumococci, which at lower concentrations shows mechanistic similarities to HAMLET's bactericidal activity. ELOA binds to S. pneumoniae and causes perturbations of the plasma membrane, including depolarization and subsequent rupture, and activates an influx of calcium into the cells. Selective inhibition of calcium channels and sodium/calcium exchange activity significantly diminished ELOA's bactericidal activity, similar to what we have observed with HAMLET. Finally, ELOA-induced death was also accompanied by DNA fragmentation into high molecular weight fragments - an apoptosis-like morphological phenotype that is seen during HAMLET-induced death. Thus, in contrast to different mechanisms of eukaryote cell death induced by ELOA and HAMLET, these complexes are characterized by rather similar activities towards bacteria. Although the majority of these events could be mimicked using oleic acid alone, the concentrations of oleic acid required were significantly higher than those present in the ELOA complex, and for some assays, the results were not identical between oleic acid alone and the ELOA complex. This indicates that the lipid, as a common denominator in both complexes, is an important component for the complexes' bactericidal activities, while the proteins are required both to solubilize and/or present the lipid at the bacterial membrane and likely to confer other and separate functions during the bacterial death.
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- Stroe, Silvia, et al.
(författare)
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The Outcomes of Passion in Entrepreneurship
- 2019
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Ingår i: Passion for work. - : Oxford University Press. - 9780190648626
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Bokkapitel (refereegranskat)abstract
- This chapter surveys the literature on the outcomes of passion in entrepreneurship. The findings reveal that passion is consequential not only with respect to intrapersonal outcomes (affect, cognition, behavior of the entrepreneur, and performance of their venture) but also for interpersonal results (for stakeholders such as investors or employees). These outcomes, however, are not always beneficial: although passion has been proven to have a host of positive outcomes in entrepreneurship, more and more recent studies have started to uncover its dualistic nature and detrimental effects. The review offers specific suggestions that will be instrumental in carrying out future research regarding the role of passion in entrepreneurship and specifies implications for entrepreneurship practitioners.
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- Wang, Bolin, et al.
(författare)
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Palladium-Catalyzed Dehydrogenative Carbonylative Esterification of Allenoic Acids for the Synthesis of γ-Butyrolactone Derivatives
- 2024
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Ingår i: Organic Letters. - 1523-7060 .- 1523-7052.
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Tidskriftsartikel (refereegranskat)abstract
- A highly efficient dehydrogenative carbonylative esterification of allenoic acids using Pd-catalysis was developed, providing a novel approach to synthesizing esterified γ-butyrolactone derivatives with consistently good to excellent results demonstrated across over 50 examples. Additionally, we used a heterogeneous catalyst known as Pd-AmP-MCF and harnessed biomimetic-aerobic-oxidation conditions to facilitate the practical execution of this reaction. Furthermore, our detailed study of γ-butyrolactone products highlighted their potential in synthesizing bioactive compounds.
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