| 251. |
- Dinér, Peter, 1976, et al.
(författare)
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Design, synthesis, and characterization of a highly effective Hog1 inhibitor: a powerful tool for analyzing MAP kinase signaling in yeast.
- 2011
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Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 6:5
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Tidskriftsartikel (refereegranskat)abstract
- The Saccharomyces cerevisiae High-Osmolarity Glycerol (HOG) pathway is a conserved mitogen-activated protein kinase (MAPK) signal transduction system that often serves as a model to analyze systems level properties of MAPK signaling. Hog1, the MAPK of the HOG-pathway, can be activated by various environmental cues and it controls transcription, translation, transport, and cell cycle adaptations in response to stress conditions. A powerful means to study signaling in living cells is to use kinase inhibitors; however, no inhibitor targeting wild-type Hog1 exists to date. Herein, we describe the design, synthesis, and biological application of small molecule inhibitors that are cell-permeable, fast-acting, and highly efficient against wild-type Hog1. These compounds are potent inhibitors of Hog1 kinase activity both in vitro and in vivo. Next, we use these novel inhibitors to pinpoint the time of Hog1 action during recovery from G(1) checkpoint arrest, providing further evidence for a specific role of Hog1 in regulating cell cycle resumption during arsenite stress. Hence, we describe a novel tool for chemical genetic analysis of MAPK signaling and provide novel insights into Hog1 action.
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| 252. |
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| 253. |
- Dinér, Peter, 1976, et al.
(författare)
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Short cut to 1,2,3-triazole-based p38 MAP kinase inhibitors via [3+2]-cycloaddition chemistry
- 2009
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Ingår i: New J. Chem.. - : Royal Society of Chemistry (RSC). - 1144-0546 .- 1369-9261. ; 33:5, s. 1010-1016
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Tidskriftsartikel (refereegranskat)abstract
- A series of 4,5-substituted 1,2,3-triazoles was synthesised via Cu(I)-catalysed azide–alkyne 1,3-dipolar [2+3]-cycloaddition reactions followed by a Suzuki coupling. The 1,2,3-triazoles were evaluated as inhibitors of the p38 MAP kinase, showing IC50 values in the high nanomolar range
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| 254. |
- Dingwell, Adam, et al.
(författare)
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Seasonal and diurnal patterns in the dispersion of SO2 from Mt. Nyiragongo
- 2016
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Ingår i: Atmospheric Environment. - : Elsevier BV. - 1873-2844 .- 1352-2310. ; 132, s. 19-29
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Tidskriftsartikel (refereegranskat)abstract
- Mt. Nyiragongo is an active volcano located in the Democratic Republic of Congo, close to the border of Rwanda and about 15 km north of the city of Goma (~ 1,000,000 inhabitants). Gases emitted from Nyiragongo might pose a persistent hazard to local inhabitants and the environment. While both ground- and satellite-based observations of the emissions exist, prior to this study, no detailed analysis of the dispersion of the emissions have been made. We have conducted a dispersion study, using a modelling system to determine the geographical distribution of SO2.A combination of a meteorological model (WRF), a Lagrangian particle dispersion model (FLEXPART-WRF) and flux data based on DOAS measurements from the NOVAC-network is used. Since observations can only be made during the day, we use random sampling of fluxes and ensemble modelling to estimate night-time emissions.Seasonal variations in the dispersion follows the migration of the Inter Tropical Convergence Zone. In June-August, the area with the highest surface concentrations is located to the northwest, and in December-February, to the southwest of the source. Diurnal variations in surface concentrations were determined by the development of the planetary boundary layer and the lake-/land breeze cycle around lake Kivu. Both processes contribute to low surface concentrations during the day and high concentrations during the night. However, the strong northerly trade winds in November-March weakened the lake breeze, contributing to higher daytime surface concentrations along the northern shore of Lake Kivu, including the city of Goma. For further analysis and measurements, it is important to include both seasonal and diurnal cycles in order to safely cover periods of high and potentially hazardous concentrations.
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| 255. |
- Doan, Lam, et al.
(författare)
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The extended molecular envelope of the asymptotic giant branch star π1 Gruis as seen by ALMA II. The spiral-outflow observed at high-angular resolution
- 2020
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 633
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Tidskriftsartikel (refereegranskat)abstract
- Context. This study is a follow up to the previous analysis of lower-angular resolution data in which the kinematics and structure of the circumstellar envelope (CSE) around the S-type asymptotic giant branch (AGB) star pi(1) Gruis were investigated. The AGB star has a known companion (at a separation of 400 AU) that cannot explain the strong deviations from spherical symmetry of the CSE. Recently, hydrodynamic simulations of mass transfer in closer binary systems have successfully reproduced the spiral-shaped CSEs found around a handful of sources. There is growing evidence for an even closer, undetected companion complicating the case of pi(1) Gruis further. Aims. The improved spatial resolution allows for the investigation of the complex circumstellar morphology and the search for imprints on the CSE of the third component. Methods. We have observed the (CO)-C-12 J = 3-2 line emission from pi(1) Gruis using both the compact and extended array of Atacama Large Millimeter/submillimeter Array (ALMA). The interferometric data have furthermore been combined with data from the ALMA total power array. The imaged brightness distribution has been used to constrain a non-local, non-local thermodynamic equilibrium 3D radiative transfer model of the CSE. Results. The high-angular resolution ALMA data have revealed the first example of a source on the AGB where both a faster bipolar outflow and a spiral pattern along the orbital plane can be seen in the gas envelope. The spiral can be traced in the low- to intermediate-velocity (13-25 km s(-1)) equatorial torus. The largest spiral-arm separation is approximate to 5.'' 5 and consistent with a companion with an orbital period of approximate to 330 yr and a separation of less than 70 AU. The kinematics of the bipolar outflow is consistent with it being created during a mass-loss eruption where the mass-loss rate from the system increased by at least a factor of five for 10-15 yr. Conclusions. The spiral pattern is the result of an undetected companion. The bipolar outflow is the result of a rather recent mass-loss eruption event.
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| 256. |
- Donatelli, M., et al.
(författare)
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Multigrid methods for cubic spline solution of two point (and 2D) boundary value problems
- 2016
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Ingår i: Applied Numerical Mathematics. - : Elsevier BV. - 0168-9274 .- 1873-5460. ; 104, s. 15-29
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we propose a scheme based on cubic splines for the solution of the second order two point boundary value problems. The solution of the algebraic system is computed by using optimized multigrid methods. In particular the transformation of the stiffness matrix essentially in a block Toeplitz matrix and its spectral analysis allow to choose smoothers able to reduce error components related to the various frequencies and to obtain an optimal method. The main advantages of our strategy can be listed as follows: (i) a fourth order of accuracy combined with a quadratic conditioning matrix, (ii) a resulting matrix structure whose eigenvalues can be compactly described by a symbol (this information is the key for designing an optimal multigrid method). Finally, some numerics that confirm the predicted behavior of the method are presented and a discussion on the two dimensional case is given, together with few 2D numerical experiments. (C) 2014 IMACS. Published by Elsevier B.V. All rights reserved.
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| 257. |
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| 258. |
- Duan, Yu Xia, et al.
(författare)
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Crystal electric field splitting and f -electron hybridization in heavy-fermion CePt2In7
- 2019
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Ingår i: Physical Review B. - : AMER PHYSICAL SOC. - 2469-9969 .- 2469-9950. ; 100:8
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Tidskriftsartikel (refereegranskat)abstract
- We use high-resolution angle-resolved photoemission spectroscopy to investigate the electronic structure of the antiferromagnetic heavy fermion compound CePt2In7, which is a member of the CeIn3-derived heavy fermion material family. Weak hybridization among 4f electron states and conduction bands was identified in CePt2In7 at low temperature much weaker than that in the other heavy fermion compounds like CeIrIn5 and CeRhIn5. The Ce 4f spectrum shows fine structures near the Fermi energy, reflecting the crystal electric field splitting of the 4f5/21 and 4f7/21 states. Also, we find that the Fermi surface has a strongly three-dimensional topology, in agreement with density-functional theory calculations. © 2019 American Physical Society.
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| 259. |
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| 260. |
- Durkin, Louisa, et al.
(författare)
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When mycologists describe new species, not all relevant information is provided (clearly enough)
- 2020
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Ingår i: MycoKeys. - : Pensoft Publishers. - 1314-4057 .- 1314-4049. ; 72, s. 109-128
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Tidskriftsartikel (refereegranskat)abstract
- Taxonomic mycology struggles with what seems to be a perpetual shortage of resources. Logically, fungal taxonomists should therefore leverage every opportunity to highlight and visualize the importance of taxonomic work, the usefulness of taxonomic data far beyond taxonomy, and the integrative and collaborative nature of modern taxonomy at large. Is mycology really doing that, though? In this study, we went through ten years' worth (2009-2018) of species descriptions of extant fungal taxa - 1,097 studies describing at most ten new species - in five major mycological journals plus one plant journal. We estimated the frequency at which a range of key words, illustrations, and concepts related to ecology, geography, taxonomy, molecular data, and data availability were provided with the descriptions. We also considered a range of science-demographical aspects such as gender bias and the rejuvenation of taxonomy and taxonomists as well as public availability of the results. Our results show that the target audience of fungal specks descriptions appears to be other fungal taxonomists, because many aspects of the new species were presented only implicitly, if at all. Although many of the parameters we estimated show a gradual, and in some cases marked, change for the better over time, they still paint a somewhat bleak picture of mycological taxonomy as a male-dominated field where the wants and needs of an extended target audience are often not understood or even considered. This study hopes to leave a mark on the way fungal species are described by putting the focus on ways in which fungal taxonomy can better anticipate the end users of species descriptions - be they mycologists, other researchers, the public at large, or even algorithms. In the end, fungal taxonomy, too, is likely to benefit from such measures.
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