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Träfflista för sökning "LAR1:lu ;pers:(Andersson Engels Stefan)"

Sökning: LAR1:lu > Andersson Engels Stefan

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  • Andersson-Engels, Stefan, et al. (författare)
  • Time-resolved and Wavelength-resolved Spectroscopy In 2-photon-excited Fluorescence Microscopy
  • 1994
  • Ingår i: Journal of Microscopy. - 0022-2720. ; 176, s. 195-203
  • Tidskriftsartikel (refereegranskat)abstract
    • Two-photon excited fluorescence spectroscopy has been performed at a microscopic scale in combination with normal, white-light microscopy. This gave simultaneously a spectral resolution of 20 nm and a temporal resolution of 20 ps, from a volume element less than 5 mu m in all three dimensions. The sample was excited with the light from a continuously mode-locked Ti:sapphire laser that was focused on the sample in a fluorescence microscope. A polychromator and a streak-camera were used for detection. The method has been used on tissue, plant and paper samples. It has also been demonstrated how substances naturally occurring in the samples can be identified from their spectroscopic properties and the spatial distribution of these substances can be observed.
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  • Andersson-Engels, Stefan, et al. (författare)
  • Tumour marking properties of different haematoporphyrins and tetrasulfonated phthalocyanine - A comparison
  • 1989
  • Ingår i: Lasers in Medical Science. - 0268-8921. ; 4:2, s. 115-123
  • Tidskriftsartikel (refereegranskat)abstract
    • Several photosensitizers were screened for their tumour-marking ability using laserinduced fluorescence in Wistar/Furth rats bearing subcutaneous adenocarcinomas inoculated in muscle. Of the studied photosensitizers, dihaematoporphyrin ether appeared to exhibit the best tumour-demarcation properties. Polyhaematoporphyrin ester and tetrasulfonated phthalocyanine were almost as good although the fluorescence yield was much lower. Monomeric haematoporphyrin also showed some tumour-marking qualities. By forming fluorescence intensity ratios, information from both the blue and the red spectral regions were used to provide the highest tumour-to-muscle contrast. Two excitation wavelengths were used, of which 337 nm rather than 405 nm excitation light seemed to yield a better tumour demarcation, due to a greater difference in the superimposing autofluorescence between tumour and surrounding tissue. The study included measurements on many inner organs in an attempt to gain a better understanding of the interaction between the drugs and various kinds of tissue.
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60.
  • Axelsson, Johan, et al. (författare)
  • In vivo photosensitizer tomography inside the human prostate
  • 2009
  • Ingår i: Optics Letters. - 0146-9592. ; 34:3, s. 232-234
  • Tidskriftsartikel (refereegranskat)abstract
    • Interstitial photodynamic therapy (IPDT) provides a promising means to treat large cancerous tumors and solid organs inside the human body. The treatment outcome is dependent on the distributions of light, photosensitizer, and tissue oxygenation. We present a scheme for reconstructing the spatial distribution of a fluorescent photosensitizer. The reconstruction is based on measurements performed in the human prostate, acquired during an ongoing IPDT clinical trial, as well as in optical phantoms. We show that in an experimental setup we can quantitatively reconstruct a fluorescent inclusion in a fluorescent background. We also show reconstructions from a patient showing a heterogeneous distribution of the photosensitizer mTHPC in the human prostate. (C) 2009 Optical Society of America
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