| 51. |
- Alijagic, Andi, 1992-, et al.
(författare)
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A Novel Nanosafety Approach Using Cell Painting, Metabolomics, and Lipidomics Captures the Cellular and Molecular Phenotypes Induced by the Unintentionally Formed Metal-Based (Nano)Particles
- 2023
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Ingår i: Cells. - : MDPI. - 2073-4409. ; 12:2
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Tidskriftsartikel (refereegranskat)abstract
- Additive manufacturing (AM) or industrial 3D printing uses cutting-edge technologies and materials to produce a variety of complex products. However, the effects of the unintentionally emitted AM (nano)particles (AMPs) on human cells following inhalation, require further investigations. The physicochemical characterization of the AMPs, extracted from the filter of a Laser Powder Bed Fusion (L-PBF) 3D printer of iron-based materials, disclosed their complexity, in terms of size, shape, and chemistry. Cell Painting, a high-content screening (HCS) assay, was used to detect the subtle morphological changes elicited by the AMPs at the single cell resolution. The profiling of the cell morphological phenotypes, disclosed prominent concentration-dependent effects on the cytoskeleton, mitochondria, and the membranous structures of the cell. Furthermore, lipidomics confirmed that the AMPs induced the extensive membrane remodeling in the lung epithelial and macrophage co-culture cell model. To further elucidate the biological mechanisms of action, the targeted metabolomics unveiled several inflammation-related metabolites regulating the cell response to the AMP exposure. Overall, the AMP exposure led to the internalization, oxidative stress, cytoskeleton disruption, mitochondrial activation, membrane remodeling, and metabolic reprogramming of the lung epithelial cells and macrophages. We propose the approach of integrating Cell Painting with metabolomics and lipidomics, as an advanced nanosafety methodology, increasing the ability to capture the cellular and molecular phenotypes and the relevant biological mechanisms to the (nano)particle exposure.
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| 52. |
- Alijagic, Andi, 1992-, et al.
(författare)
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Immunotoxic, genotoxic, and endocrine disrupting impacts of polyamide microplastic particles and chemicals
- 2024
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Ingår i: Environment International. - : Elsevier. - 0160-4120 .- 1873-6750. ; 183
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Tidskriftsartikel (refereegranskat)abstract
- Due to their exceptional properties and cost effectiveness, polyamides or nylons have emerged as widely used materials, revolutionizing diverse industries, including industrial 3D printing or additive manufacturing (AM). Powder-based AM technologies employ tonnes of polyamide microplastics to produce complex components every year. However, the lack of comprehensive toxicity assessment of particulate polyamides and polyamide-associated chemicals, especially in the light of the global microplastics crisis, calls for urgent action. This study investigated the physicochemical properties of polyamide-12 microplastics used in AM, and assessed a number of toxicity endpoints focusing on inflammation, immunometabolism, genotoxicity, aryl hydrocarbon receptor (AhR) activation, endocrine disruption, and cell morphology. Specifically, microplastics examination by means of field emission scanning electron microscopy revealed that work flow reuse of material created a fraction of smaller particles with an average size of 1-5 µm, a size range readily available for uptake by human cells. Moreover, chemical analysis by means of gas chromatography high-resolution mass spectrometry detected several polyamide-associated chemicals including starting material, plasticizer, thermal stabilizer/antioxidant, and migrating slip additive. Even if polyamide particles and chemicals did not induce an acute inflammatory response, repeated and prolonged exposure of human primary macrophages disclosed a steady increase in the levels of proinflammatory chemokine Interleukin-8 (IL-8/CXCL-8). Moreover, targeted metabolomics disclosed that polyamide particles modulated the kynurenine pathway and some of its key metabolites. The p53-responsive luciferase reporter gene assay showed that particles per se were able to activate p53, being indicative of a genotoxic stress. Polyamide-associated chemicals triggered moderate activation of AhR and elicited anti-androgenic activity. Finally, a high-throughput and non-targeted morphological profiling by Cell Painting assay outlined major sites of bioactivity of polyamide-associated chemicals and indicated putative mechanisms of toxicity in the cells. These findings reveal that the increasing use of polyamide microplastics may pose a potential health risk for the exposed individuals, and it merits more attention.
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| 53. |
- Anders, Berntson, et al.
(författare)
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Self-phase modulation in dispersion compensated optical fibre transmission systems
- 1996
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Ingår i: Optics Communications. - : Elsevier. - 0030-4018 .- 1873-0310. ; 130:1-3, s. 153-162
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Tidskriftsartikel (refereegranskat)abstract
- An analysis is made of the influence of self-phase modulation on optical fibre communication systems where the chromatic dispersion is compensated for by a dispersion compensation fibre. In particular we derive a simple and useful expression for the optimal fibre length in the presence of nonlinearities.
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| 54. |
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| 55. |
- Andersson, Dan, 1966, et al.
(författare)
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Wave-breaking-free pulses in nonlinear-optical fibers
- 1993
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Ingår i: Journal of the Optical Society of America B: Optical Physics. - 1520-8540 .- 0740-3224. ; 10:7, s. 1185-1190
-
Tidskriftsartikel (refereegranskat)abstract
- A qualitative as well as quantitative investigation is made of the conditions for avoiding wave breaking during pulse propagation in optical fibers. In particular, it is shown that pulses having a parabolic intensity variation are approximate wave-breaking-free solutions of the nonlinear Schrodinger equation in the high-intensity limit. A simple expression for the compression factor of a fiber-grating compressor based on parabolic pulses is also derived.
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| 56. |
- Andrekson, Peter, 1960, et al.
(författare)
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Fiber-based phase-sensitive optical amplifiers and their applications
- 2020
-
Ingår i: Advances in Optics and Photonics. - 1943-8206. ; 12:2, s. 367-428
-
Tidskriftsartikel (refereegranskat)abstract
- Optical parametric amplifiers rely on second-order susceptibility (three-wave mixing) or third-order susceptibility (four-wave mixing) in a nonlinear process where the energy of incoming photons is not changed (elastic scattering). In the latter case, two pump photons are converted to a signal and to an idler photon. Under certain conditions, related to the phase evolution of the waves involved, this conversion can be very effi-cient, resulting in large amplification of an input signal. As the nonlinear process can be very fast, all-optical applications aside from pure amplification are also possible. If the amplifier is implemented in an optical input-phase-sensitive manner, it is possible to amplify a signal wave without excess noise, i.e., with a noise figure of 0 dB. In this paper, we will provide the fundamental concepts and theory of such amplifiers, with a focus on their implementation in highly nonlinear optical fibers relying on four-wave mixing. We will discuss the distinctions between phase-insensitive and phase-sensitive operation and include several experimental results to illustrate their capability. Different applications of parametric amplifiers are also discussed, including their use in optical communication links.
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| 57. |
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| 58. |
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| 59. |
- Ankiewicz, A., et al.
(författare)
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Dark soliton pairs in fiber couplers
- 1994
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Ingår i: Optics Communications. - 0030-4018. ; 111:1-2, s. 116-122
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Tidskriftsartikel (refereegranskat)abstract
- We analyze the possible stationary states formed by pairs of dark solitons propagating in dual-core optical fibers, i.e. in a nonlinear coupler. These are local disturbances on a constant background. We find new dark soliton states, characterize them and show that they differ from the soliton states consisting of coupled bright solitons in several ways. © 1994.
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| 60. |
- Bakhshi, Bamdad, 1970, et al.
(författare)
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Experimental observation of soliton robustness to polarisation dispersion pulse broadening
- 1999
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Ingår i: Electronics Letters. - : Institution of Engineering and Technology (IET). - 1350-911X .- 0013-5194. ; 35:1, s. 65-66
-
Tidskriftsartikel (refereegranskat)abstract
- Soliton robustness to polarisation dispersion pulse broadening in a 400km installed optical fibre is experimentally demonstrated. With 10ps input pulses and a differential group delay of 7.6ps, the soliton pulsewidth at the receiver is maintained within 10-11ps when the input polarisation is changed, while the corresponding range for dispersion-compensated linear pulses is 10.5-15.5ps.
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