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- Liu, Wen-Chun, et al.
(författare)
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Genotyping of Hepatitis B Virus - Genotypes A to G by Multiplex Polymerase Chain Reaction
- 2008
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Ingår i: Intervirology. - : S. Karger AG. - 0300-5526 .- 1423-0100. ; 51:4, s. 247-252
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Tidskriftsartikel (refereegranskat)abstract
- <i>Objectives:</i> Eight genotypes (A–H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples. <i>Methods:</i> To establish a simple and accurate genotyping method, the study collected 369 HBV complete genomic sequences from the GenBank database. Type-specific primers were also designed that separated HBV genotypes A to G by multiplex polymerase chain reaction. <i>Results:</i> By comparison with the traditional restriction fragment length polymorphism method, over 93% of 441 samples were accurately genotyped by current assay, with a higher detection rate and sensitivity to detect mixed HBV infections. <i>Conclusions:</i> This methodology can be applied only to areas prevalent with HBV genotypes A to G. However, it provides an efficient alternative for clinical diagnosis and large-scale studies.
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