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- Duong-Thi, Minh-Dao, et al.
(författare)
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Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins
- 2016
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Ingår i: The Analyst. - 0003-2654 .- 1364-5528. ; 141:3, s. 981-988
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Tidskriftsartikel (refereegranskat)abstract
- Membrane proteins constitute the largest class of drug targets but they present many challenges in drug discovery. Importantly, the discovery of potential drug candidates is hampered by the limited availability of efficient methods for screening drug-protein interactions. In this work we present a novel strategy for rapid identification of molecules capable of binding to a selected membrane protein. An integral membrane protein (human aquaporin-1) was incorporated into planar lipid bilayer disks (lipodisks), which were subsequently covalently coupled to porous derivatized silica and packed into HPLC columns. The obtained affinity columns were used in a typical protocol for fragment screening by weak affinity chromatography (WAC), in which one hit was identified out of a 200 compound collection. The lipodisk-based strategy, which ensures a stable and native-like lipid environment for the protein, is expected to work also with other membrane proteins and screening procedures.
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| 3. |
- Kero, Anneli, 1950-, et al.
(författare)
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Kvinnors och mäns upplevelser av abort
- 2018
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Ingår i: Inducerad abort. - Stockholm : Svensk förening för obstetrik och gynekologi. ; , s. 44-46
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 6. |
- Wanders, A., et al.
(författare)
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Ischemia-induced transplant arteriosclerosis in the rat
- 1995
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Ingår i: Arterioscler Thromb Vasc Biol. ; 15:1, s. 145-55
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Tidskriftsartikel (refereegranskat)abstract
- The effect of cold graft ischemia time on the development of transplant arteriosclerosis was investigated. Aorta grafts from DA or PVG rats were stored in a cold perfusion solution for 1, 4, or 24 hours before being orthotopically transplanted to PVG recipients. After observation times ranging from 2 to 8 weeks, the grafts were examined for various cell populations. Regional changes in the intima and media layers were measured by using an image analysis system. The arteriosclerosis-like changes seen in syngeneic grafts with the longest ischemia time could be almost as prominent as those seen in the allogeneic transplants. The magnitude of the regional intima changes in the syngeneic group correlated well with the ischemia time and in the allogeneic group with the observation time. The cell composition found in the intima and media of the allogeneic vessels consisted of macrophages, T-lymphocytes, MHC class II-expressing cells, and smooth muscle cells, whereas the syngeneic grafts contained almost exclusively smooth muscle cells and macrophages. We therefore conclude that the damage due to prolonged cold ischemia time is sufficient to cause pronounced graft arteriosclerosis. The pathophysiological mechanism leading to ischemia-induced arteriosclerosis is different from the one seen in the allogeneic situation.
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| 9. |
- Chaudhary, Ramjee, et al.
(författare)
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Kinetic study of nutrients removal from municipal wastewater by Chlorella vulgaris in photobioreactor supplied with CO2-enriched air
- 2020
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Ingår i: Environmental technology. - : TAYLOR & FRANCIS LTD. - 0959-3330 .- 1479-487X. ; 41:5, s. 617-626
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Tidskriftsartikel (refereegranskat)abstract
- The microalgae Chlorella vulgaris ATCC 13482 was used in the present study for municipal wastewater treatment. Batch experiments were performed in bubble column photobioreactors of 7 L working volume maintained at 25 +/- 2 degrees C and 14 h/10 h of photo and dark cycle. The treatment process was enhanced by using CO2-augmented air (5% CO2 v/v) supply into the microalgal culture in comparison to the use of normal air (0.03% CO2 v/v). For a period of 7 days, C. vulgaris effected maximum removals of 74.4% soluble fraction of chemical oxygen demand, 72% ammonia (NH4-N), 60% nitrate (NO3-N) and 81.93% orthophosphate (PO4-P) with use of normal air, whereas 84.6% sCOD, 88% NH4-N, 72% NO3-N and 92.8% PO4-P removals, respectively, with use of 5% CO2/air supply. Using kinetic study data, the specific rates of ammonia and phosphate uptake (q(ammonia) and q(phosphate)) by C. vulgaris at 5% CO2/air supply were found to be 2.41 and 0.85 d(-1), respectively. Using the algal remediation technology, nitrogen-phosphorus-potassium recovery from sewage treatment plant of 37.5 million litres per day wastewater influent capacity was calculated to be similar to 298.5, 55.4 and 83.7 kg d(-1), respectively.
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| 10. |
- Wanders, A., et al.
(författare)
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Expression of an interferon-gamma-like substance in normal and transplanted rat heart tissue
- 1992
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Ingår i: J Heart Lung Transplant. ; 11:1 Pt 1, s. 142-6
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Tidskriftsartikel (refereegranskat)abstract
- The presence of interferon-gamma-like molecules has been reported not only in lymphocytes, but also in certain nerve cells and in normal skeletal muscle. We have studied the reactivity of the anti-interferon-gamma monoclonal antibody DB1 with frozen sections of normal and transplanted rat hearts. Cardiac grafts from PVG donor rats were transplanted to syngeneic PVG recipients or allogeneic Wistar/Kyoto recipient rats with the use of an accessory cervical heart transplantation technique. The allogeneic heart transplants were harvested 4 days and the syngeneic grafts 4 weeks after transplantation. In normal hearts there was a weak but distinct reactivity with the anti-interferon-gamma antibody in most muscle cells. In addition, some lymphocytes and the Purkinje fibers were positive. Hearts transplanted over an allogeneic barrier revealed that staining for interferon-gamma on muscle cells was substantially increased whereas no or only a moderate increase in the anti-interferon-gamma staining was seen in hearts transplanted to syngeneic recipients. These data indicate that interferon-gamma present in rat myocyte may be involved in the pathophysiology of graft rejection and also suggest that interferon-gamma may be of importance for the function of normal rat heart muscle cells.
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