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Träfflista för sökning "WFRF:(Nilsson Patrik) srt2:(1995-1999)"

Sökning: WFRF:(Nilsson Patrik) > (1995-1999)

  • Resultat 1-9 av 9
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  • Nilsson, Patrik W. (författare)
  • The West Ny Friesland Terrane : An exhumed mid-crustal obliquely convergent orogen : by Patrik W. Nilsson
  • 1998
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Ny Friesland, in northeastern Svalbard, is the type-area for transpressive orogeny. The West Ny Friesland Terrane (WNT) is composed of a c. 12 km thick basal succession of amphibolite-grade schists and gneisses, previously thought to be the lower of a c. 20 km thick succession of concordant, volcano-sedimentary strata. However, this lower succession is composed of at least five thrust intercalations, where four contain Palaeoproterozoic (c. 1750 Ma) basement gneisses and Mesoproterozoic (or younger) cover sequences. Concordance between the rock units is largely the result of ductile shearing and high Caledonian strain during thrusting. These thrust sheets were assembled sequentially during E-W orthogonal shortening ( minimum c. 80 km) and resulted in a c. 150 km long, N-S trending fold-and-thrust belt, the Atomfjella Antiform, in Ny Friesland, northeastern Spitsbergen. The geometry of this structure, with thinning of some units in the east, suggests that nappe transport was west-directed. Shortening was coupled with increasing grade of metamorphism, and changes in both vertical and lateral boundary conditions during increasing sinistral transpression. This resulted in ductile hinge-parallel elongation of the thrust-stack.Comparison of geochronological and structural data from Ny Friesland with data from the Nordauslandet Terrane (NAT) indicates that these eastern areas differ fundamentally. The Palaeoproterozoic granites in Ny Friesland have no counterparts in Nordaustlandet which is dominated by a Grenvillian-age tectonothermal history. The metamorphic grade is also lower in Nordaustlandet. The NAT Neoproterozoic and Early Palaeozoic strata show affinity with the successions of Central-East Greenland. The Ny Friesland Palaeoproterozoic gneisses are similar in age to the high-grade gneisses and metasediments in North-East Greenland, suggesting a close affinity with these areas. This thesis proposes that the WNT was the result of Caledonian transpressional deformation during collision between Baltica and Laurentia, and that tectonic escape had juxtaposed the WNT and NAT terranes north of Greenland by the end of the Silurian
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  • Årzén, Karl-Erik, et al. (författare)
  • Integrated Control and Scheduling
  • 1999
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • The report gives a state-of-the-art survey of the field of integrated control and scheduling. Subtopics discussed are implementation and scheduling of periodic control loops, scheduling under overload, control and scheduling co-design, dynamic task adaptation, feedback scheduling, and scheduling of imprecise calculations. The report also presents the background, motivation, and research topics in the ARTES project Integrated Control and Scheduling.
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  • Önnerfjord, Patrik, et al. (författare)
  • Homogeneous sample preparation for automated high throughput analysis with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
  • 1999
  • Ingår i: Rapid Communications in Mass Spectrometry. - 0951-4198 .- 1097-0231. ; 13:5, s. 315-22
  • Tidskriftsartikel (refereegranskat)abstract
    • This work presents a simple method for obtaining homogeneous sample surfaces in matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the automated analysis of peptides and proteins. The sample preparation method is based on applying the sample/matrix mixture onto a pre-deposited highly diluted matrix spot. The pre-deposited crystals act as seeds for the new sample containing crystals which become much smaller in size and more evenly distributed than with conventional methods. This 'seed-layer' method was developed, optimised and compared with the dried-droplet method using peptides and proteins in the 1000-20,000 Da range. The seed-layer method increases the surface homogeneity, spot to spot reproducibility and sample washability as compared with the commonly used dried-droplet method. This methodology is applicable to alpha-cyanohydroxycinnamic acid, sinapinic acid and ferulic acid, which all form homogeneous crystal surfaces. Within-spot variation and between-spot variation was investigated using statistics at a 95% confidence level (n = 36). The statistical values were generated from more than 5000 data points collected from 500 spectra. More than 90% of the sample locations results in high intensity spectra with relatively low standard deviations (RSDs). Typically obtained data showed an RSD of 19-35% within a sample spot as well as in-between spots for proteins, and an RSD of < or = 50% for peptides. Linear calibration curves were obtained within one order of magnitude using internal calibration with a point-RSD of 3% (n = 10). The sample homogeneity allows mass spectra (average of 16 laser shots) to be obtained on each individual sample within 15 sec, whereby a 100 spot target plate can be run in 25 min. High density target plates using the seed-layer method were prepared by spotting approximately 100 picoliter droplets onto the target, resulting in sample spots < or = 500 microns in diameter using a flow-through piezo-electric micro-dispenser. By using this automated sample preparation step lower standard deviations are obtained in comparison to manually prepared samples.
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  • Önnerfjord, Patrik, et al. (författare)
  • Picoliter sample preparation in MALDI-TOF MS using a micromachined silicon flow-through dispenser
  • 1998
  • Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 70:22, s. 4755-4760
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper presents a picoliter sample preparation technique utilizing the flow-through principle, allowing on-line coupling of chromatographic systems to be made. The work was performed in order to investigate the characteristics and the physicochemical properties of the sample preparation using typical mobile phase conditions from μ-CLC (column liquid chromatography) separations. The device presented here is a pressure pulse- driven dispenser, formed by two silicon structures processed by conventional micromachining. The pressure pulse is generated in the flow-through channel by a piezoceramic element. Depending on the orifice size, the droplets ejected range between 30 and 200 pL. The maximum ejection frequency is 500 Hz, limited by resonances within the unit. A pyramid-shaped nozzle improves the directivity of the droplets since it reduces the wetting of the orifice front surface area. The risk of particles sticking close to the orifice is also minimized. The analyses of the deposited sample spots were carried out on a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer with delayed extraction. It was possible to detect attomole amounts (159-248 amol) of various proteins (cytochrome c, ribonuclease A, lysozyme, and myoglobin) from a single droplet of matrix:analyte 1:1 (drop volume ≃ 110 pL). Additionally, it was found that sample enrichment could be carried out using multiple depositions on the same spot; i.e., 31 nM of insulin was easily detected when more than four depositions were made on the same spot, while no detection was possible without sample enrichment. Size optimization of the MALDI sample spot gave target zones of 100-500-μm diameter that matched the size of the laser focal point and resulted in a considerably increased sample throughput.
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