| 151641. |
- Lomidze, Levan, et al.
(författare)
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Calibration and Validation of Swarm Plasma Densities and Electron Temperatures Using Ground-Based Radars and Satellite Radio Occultation Measurements
- 2018
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Ingår i: Radio Science. - 0048-6604 .- 1944-799X. ; 53:1, s. 15-36
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Tidskriftsartikel (refereegranskat)abstract
- In this study we calibrate and validate in situ ionospheric electron density (N-e) and temperature (T-e) measured with Langmuir probes (LPs) on the three Swarm satellites orbiting the Earth in circular, nearly polar orbits at similar to 500km altitude. We assess the accuracy and reliability of the LP data (December 2013 to June 2016) by using nearly coincident measurements from low- and middle-latitude incoherent scatter radars (ISRs), low-latitude ionosondes, and Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC) satellites, covering all latitudes. The comparison results for plasma frequency (f alpha root Ne) for each Swarm satellite are consistent across these three, principally different measurement techniques. It shows that the Swarm LPs systematically underestimate plasma frequency by about 10% (0.5-0.6MHz). The correlation coefficients are high (0.97), indicating accurate relative variation in the Swarm LP densities. The comparison of T-e from high-gain LPs and those from ISRs reveals that all three satellites overestimate it by 300-400K but exhibit high correlations (0.92-0.97) against the validation data. The low-gain LP T-e data show larger overestimation (similar to 700K) and lower correlation (0.86-0.90). The adjustment of the Swarm LP data based on Swarm-ISR comparison results removes the systematic biases in the Swarm data and gives plasma frequencies and high- and low-gain electron temperatures that are precise within about 0.4MHz (8%), 150-230K, and 260-360K, respectively. We demonstrate that the applied correction significantly improves the agreement between (1) the plasma densities from Swarm, and from ionosondes and COSMIC, and (2) the T-e from Swarm LPs and International Reference Ionosphere 2016.
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| 151642. |
- Lomniczi B., Wehmann E., Herczeg J., Ballagi-Pordany, A., Kaleta, E.F., Werner, O., Meulemans, G., Jorgensen, P.H., Mante, A.P., Gielkens, A.L., Capua, I., and Damoser, J.
(författare)
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Newcastle disease outbreaks in recent years in western Europe were caused by an old (VI) and a novel genotype (VII)
- 1998
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Ingår i: Arch Virol. ; 143:1, s. 49-64
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Tidskriftsartikel (refereegranskat)
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| 151643. |
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| 151644. |
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| 151645. |
- Lomnytska, Marta I, et al.
(författare)
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Diagnostic protein marker patterns in squamous cervicalcancer
- 2010
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Ingår i: Proteomics - Clinical Applications. - Weinheim : WILEY-VCH Verlag GmbH & Co. KGaA. - 1862-8346 .- 1862-8354. ; 4:1, s. 17-31
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Cervical cancer is the second most prevalent malignancy of women. Our aim was toidentify additional marker protein patterns for objective diagnosis of squamous cervical cancer(SCC).Experimental design: Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normalcervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MSprotein identification, and analysis of the expression of selected proteins by immunohistochemistry.Results: In 148 protein spots selected by the difference in expression 99 proteins were identified.A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translationinitiation factor 3-2b, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OEcathepsin D, g-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3,HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression ofneutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) wasobserved while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53)agreement between the expression of protein spots and the immune expression of proteins(www.proteinatlas.org).Conclusions and clinical relevance: SCC is characterized by specific tissue marker proteinpatterns that allow objective detection of the disease. They can become a basis for objectiveautomated cytology-based screening and improve current diagnostics of SCC.
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| 151646. |
- Lomnytska, M. I., et al.
(författare)
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Differential expression of ANXA6, HSP27, PRDX2, NCF2, and TPM4 during uterine cervix carcinogenesis : diagnostic and prognostic value
- 2011
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Ingår i: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 104:1, s. 110-119
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Tidskriftsartikel (refereegranskat)abstract
- Background:Cytology-based diagnostics of squamous cervical cancer (SCC) precursor lesions is subjective and can be improved by objective markers.Methods:IHC-based analysis of ANXA6, HSP27, peroxiredoxin 2 (PRDX2), NCF2, and tropomyosin 4 (TPM4) during SCC carcinogenesis.Results:Expression of ANXA6, HSP27, PRDX2, and NCF2 in the cytoplasm of dysplastic cells increased from cervical intraepithelial neoplasia 2/3 (CIN2/3) to microinvasive cancer. Invasive SCC showed lower expression of TPM4 than CIN and normal epithelium. CIN2/3 with the highest sensitivity and specificity differed from normal epithelium by cytoplasmic expression of HSP27. Patients with cytoplasmic HSP27 expression in SCC deviating from that observed in normal epithelium had worse relapse-free (P0.019) and overall (P0.014) survival. Invasive SCC with the highest sensitivity and specificity differed from normal epithelium by expression of PRDX2 and TPM4 in the cytoplasm, from CIN2/3 by the expression of ANXA6 and TPM4 in the cytoplasm, and from microinvasive SCC by the expression of PRDX2 and ANXA6 in the cytoplasm. The number of sporadic ANXA6 cells between the atypical cells increased from CIN2/3 to invasive SCC.Conclusion:Detection of expression changes of the proteins ANXA6, HSP27, PRDX2, NCF2, and TPM4 in SCC precursor lesions may aid current cytological and pathological diagnostics and evaluation of prognosis.
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| 151647. |
- Lomnytska, M. I., et al.
(författare)
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Impact of genomic stability on protein expression in endometrioid endometrial cancer
- 2012
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Ingår i: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 106:7, s. 1297-1305
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Genomic stability is one of the crucial prognostic factors for patients with endometrioid endometrial cancer (EEC). The impact of genomic stability on the tumour tissue proteome of EEC is not yet well established.METHODS: Tissue lysates of EEC, squamous cervical cancer (SCC), normal endometrium and squamous cervical epithelium were subjected to two-dimensional (2D) gel electrophoresis and identification of proteins by MALDI TOF MS. Expression of selected proteins was analysed in independent samples by immunohistochemistry.RESULTS: Diploid and aneuploid genomically unstable EEC displayed similar patterns of protein expression. This was in contrast to diploid stable EEC, which displayed a protein expression profile similar to normal endometrium. Approximately 10% of the differentially expressed proteins in EEC were specific for this type of cancer with differential expression of other proteins observed in other types of malignancy (e.g., SCC). Selected proteins differentially expressed in 2D gels of EEC were further analysed in an EEC precursor lesion, that is, atypical hyperplasia of endometrium, and showed increased expression of CLIC1, EIF4A1 and PRDX6 and decreased expression of ENO1, ANXA4, EMD and Ku70.CONCLUSION: Protein expression in diploid and aneuploid genomically unstable EEC is different from the expression profile of proteins in diploid genomically stable EEC. We showed that changes in expression of proteins typical for EEC could already be detected in precursor lesions, that is, atypical hyperplasia of endometrium, highlighting their clinical potential for improving early diagnostics of EEC.
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| 151648. |
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| 151649. |
- Lomnytska, Marta, et al.
(författare)
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Increased expression of cSHMT, Tbx3 and utrophin in plasma of ovarian and breast cancer patients
- 2006
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 118:2, s. 412-421
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Tidskriftsartikel (refereegranskat)abstract
- Plasma samples of ovarian and breast cancer patients were used to search for markers of cancer using 2-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Truncated forms of cytosolic serine hydroxymethyl transferase (cSHMT), T-box transcription factor 3 (Tbx3) and utrophin were aberrantly expressed in samples from cancer patients as compared to samples from noncancerous cases. Aberrant expression of proteins was validated by immunoblotting of plasma samples with specific antibodies to cSHMT, Tbx3 and utrophin. A cohort of 79 breast and 39 ovarian cancer patients and 31 individuals with noncancerous conditions was studied. We observed increased expression of truncated cSHMT, Tbx3 and utrophin in plasma samples obtained from patients at early stages of disease. Our data suggest that cSHMT, Tbx3 and utrophin can be used as components of multiparameter monitoring of ovarian and breast cancer (supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html).
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| 151650. |
- Lomnytska, Marta, PhD, 1979-, et al.
(författare)
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Peritoneal cancer index predicts severe complications after ovarian cancer surgery
- 2021
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Ingår i: European Journal of Surgical Oncology. - : Elsevier. - 0748-7983 .- 1532-2157. ; 47:11, s. 2915-2924
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Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION: prediction and importance of severe postoperative complications after ovarian cancer surgery is a strong issue in patient selection and evaluation. Pre- and early peroperative predictors of severe 30-days postoperative complications (Clavien-Dindo class ≥3) after surgery for primary ovarian cancer are not fully established, neither their impact on patients' survival.MATERIALS AND METHODS: A prospective observational study included 256 patients with primary ovarian cancer FIGO stages IIB-IV, operated during 2009-2018 in a primary or interval debulking surgery setting. Patient variables were analysed in relation to severe postoperative complications (Clavien-Dindo class ≥3) and overall survival.RESULTS: High-grade postoperative complications occurred in 24.2% patients. Class 3a complications were observed in 12.5% cases. High-grade complications class ≥3 were observed in 31.6% after primary debulking surgery compared to 12.2% after interval debulking surgery (p = 0.0004). Peritoneal cancer index ≥21 and preoperative albumin concentration ≤33 g/L were independent predictors of high-grade complications. Peritoneal cancer index correlated with the surgical complexity score and completeness of cytoreduction. Increased peritoneal cancer index was a negative predictor of overall survival, but high-grade complications did not influence survival negatively.CONCLUSIONS: Peritoneal cancer index ≥21 was an independent predictor of high-grade complications after ovarian cancer surgery. Increased peritoneal cancer index also impacted overall survival negatively, but high-grade complications did not influence overall survival.
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