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Interleukin-13 inhi...
Interleukin-13 inhibits cell proliferation and stimulates interleukin-6 formation in isolated human osteoblasts.
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Frost, Anders (author)
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Jonsson, K B (author)
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Brändström, Helena (author)
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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Ljunghall, S (author)
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Ljunggren, O (author)
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(creator_code:org_t)
- 1998
- 1998
- English.
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In: The Journal of clinical endocrinology and metabolism. - 0021-972X. ; 83:9, s. 3285-9
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Interleukin-13 (IL-13) is a recently identified cytokine that is secreted by activated T cells and regulates inflammatory responses. We have investigated the effects of IL-13 on isolated human osteoblast-like cells (hOB). IL-13 dose-dependently (1-100 pmol/L) reduced the incorporation rate of [3H]thymidine in hOB cells by more than 50%. Using a cell metabolic assay as well as direct cell counting, we found that treatment with IL-13 lead to a decrease in hOB cell number. The effect was both time and dose dependent, and after 12 days of culture, treatment with IL-13 (0.1 nmol/L) caused a 70% decrease in the number of cells. Also, IL-13 increased the levels of IL-6 messenger ribonucleic acid in hOBs, as measured by ribonuclease protection assay, and stimulated secretion of IL-6 into culture supernatants. In conclusion, IL-13 inhibits cell proliferation and increases IL-6 formation in human osteoblasts. Our findings suggest that IL-13 may cause bone loss due to impaired osteoblastic growth and IL-6-induced osteoclast recruitment.
Keyword
- Cell Division
- DNA
- biosynthesis
- Humans
- Interleukin-13
- pharmacology
- Interleukin-6
- biosynthesis
- genetics
- secretion
- Kinetics
- Osteoblasts
- cytology
- metabolism
- Osteosarcoma
- RNA
- Messenger
- metabolism
- Tumor Cells
- Cultured
Publication and Content Type
- ref (subject category)
- art (subject category)
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