| 11. |
- Andersson, Alma, et al.
(författare)
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Single-cell and spatial transcriptomics enables probabilistic inference of cell type topography
- 2020
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Ingår i: Communications Biology. - : Nature Research. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- The field of spatial transcriptomics is rapidly expanding, and with it the repertoire of available technologies. However, several of the transcriptome-wide spatial assays do not operate on a single cell level, but rather produce data comprised of contributions from a – potentially heterogeneous – mixture of cells. Still, these techniques are attractive to use when examining complex tissue specimens with diverse cell populations, where complete expression profiles are required to properly capture their richness. Motivated by an interest to put gene expression into context and delineate the spatial arrangement of cell types within a tissue, we here present a model-based probabilistic method that uses single cell data to deconvolve the cell mixtures in spatial data. To illustrate the capacity of our method, we use data from different experimental platforms and spatially map cell types from the mouse brain and developmental heart, which arrange as expected.
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| 12. |
- Andersson, Mats X., 1977, et al.
(författare)
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Optical manipulation reveals strong attracting forces at membrane contact sites between endoplasmic reticulum and chloroplasts
- 2007
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Ingår i: Journal of Biological Chemistry. - 0021-9258. ; 282:2, s. 1170-1174
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Tidskriftsartikel (refereegranskat)abstract
- Eukaryote cells depend on membrane lipid trafficking from biogenic membranes, like the endoplasmic reticulum (ER), to other membranes in the cell. Two major routes for membrane lipid transport are recognized: vesicular trafficking and lipid transfer at zones of close contact between membranes. Specific ER regions involved in such membrane contact sites (MCSs) have been isolated, and lipid transfer at MCSs as well as protein-protein interactions between the partaking membranes have been demonstrated (reviewed by Holthuis, J. C. M., and Levine, T. P. (2005) Nat. Rev. 6, 209–220). Here we present the first demonstration of the physical association between membranes involved in MCSs: by using optical imaging and manipulation, strong attracting forces between ER and chloroplasts are revealed. We used Arabidopsis thaliana expressing green fluorescent protein in the ER lumen and observed leaf protoplasts by confocal microscopy. The ER network was evident, with ER branch end points apparently localized at chloroplast surfaces. After rupture of a protoplast using a laser scalpel, the cell content was released. ER fragments remained attached to the released chloroplasts and could be stretched out by optical tweezers. The applied force, 400 pN, could not drag a chloroplast free from its attached ER, which could reflect protein-protein interactions at the ER-chloroplast MCSs. As chloroplasts rely on import of ER-synthesized lipids, we propose that lipid transfer occurs at these MCSs. We suggest that lipid transfer at the MCSs also occurs in the opposite direction, for example to channel plastid-synthesized acyl groups to supply substrates for ER-localized synthesis of membrane and storage lipids.
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| 13. |
- Andersson, Malin, et al.
(författare)
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Yrkesfiskare i- och på marginalen: : fiske, hummersafaris och snöskottning
- 2022
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Ingår i: Arbetets och försörjningens utmarker. En antologi.
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Tjänstesamhället och den tjänsteinriktade ekonomin som växt fram under efterkrigstiden (Yarmolinsky 1968), innebar nya förhållningssätt, krav och villkor för traditionella yrkesgrupper. En händelse som pandemin ritade ytterligare om förhållningssätt och normer kring arbetet. Varje tid har således sina föreställningar om arbete, vad som är en lämplig anställd eller en bra chef. Varför sker sådana normförskjutningar och vad händer i dessa förändringsprocesser? För att förstå hur ett traditionellt yrke navigerar i samtiden, reflekterar denna text kring förändring med fokus på en urgammal men fortfarande verksam yrkesgrupp, nämligen kustnära yrkesfiskare. Ett kustnära yrkesfiske sker, som namnet antyder, nära land och genomförs under en och samma dag. Till skillnad från ett yrkesfiske som sker på stora båtar långt ute till havs under flera dagar i sträck, så innebär det kustnära yrkesfisket att fiskaren kan leverera kringtjänster som är relaterade till fisket. De kan vända sig direkt till besökare och kunder i hamnen, med till exempel fisketurer eller mat- och kulturupplevelser.
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| 14. |
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| 15. |
- Andersson, N. E., et al.
(författare)
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Methodological considerations on the determination of the APC response in plasma
- 1995
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Ingår i: Infusionstherapie und Transfusionsmedizin. - : S. Karger AG. - 1019-8466. ; 22:SUPPL. 1, s. 80-82
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Tidskriftsartikel (refereegranskat)abstract
- The performance of COATEST® APC® Resistance on different coagulation instruments has been further evaluated through analysis of plasma from 100 blood donors on KC 10, ST 4, ACL 300R, Electra 900 and Thrombolyzer. The electromechanical instruments KC-10 and ST 4 showed a lower response to APC than the turbidimetric or photometric instruments with median APC ratios of 2.7 and 2.8 for the two former versus 3.1, 3.4 and 3.5, respectively, for the other three, which is in agreement with earlier initial findings. Similarly, the cut-off value varied between 2.1 and 2.6 for these instruments. The correlation of APC ratios between instruments was strong with r values ranging between 0.71 and 0.93 and, furthermore, none of the six plasmas with the lowest APC ratios on the Thrombolyzer ranked higher than 8 on any of the other instruments. Analysis of control plasmas with six consecutive kit batches on ACL and ST4 resulted in APC ratio ranges of 3.3-3.7 and 2.7-3.0 for a normal control and 1.8-2.0 and 1.9-2.0 for an abnormal control on ACL and ST4, respectively, illustrating a high reproducibility between batches. Repeated freezing and thawing of samples is disrecommended since this often resulted in increased APC ratios. In contrast, in spite of up to 40% decrease in FVIII activity upon storage of 10 different plasma samples for 5 h, the effect on the APC ratio was only minor as was also the effect of addition of 1.0 IU/ml of FVIII. In neither case was any sample misclassified. Altogether, the results support the applicability of this kit for measuring the response of plasma to APC.
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| 16. |
- Andersson, Niklas, 1970, et al.
(författare)
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Investigation of central versus peripheral effects of estradiol in ovariectomized mice
- 2005
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Ingår i: J Endocrinol. - : Bioscientifica. - 0022-0795 .- 1479-6805. ; 187:2, s. 303-9
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Tidskriftsartikel (refereegranskat)abstract
- It is generally believed that estrogens exert their bone sparing effects directly on the cells within the bone compartment. The aim of the present study was to investigate if central mechanisms might be involved in the bone sparing effect of estrogens. The dose-response of central (i.c.v) 17beta-estradiol (E2) administration was compared with that of peripheral (s.c.) administration in ovariectomized (ovx) mice. The dose-response curves for central and peripheral E2 administration did not differ for any of the studied estrogen-responsive tissues, indicating that these effects were mainly peripheral. In addition, ovx mice were treated with E2 and/or the peripheral estrogen receptor antagonist ICI 182,780. ICI 182,780 attenuated most of the estrogenic response regarding uterus weight, retroperitoneal fat weight, cortical BMC and trabecular bone mineral content (P<0.05). These findings support the notion that the primary target tissue that mediates the effect of E2 on bone is peripheral and not central.
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| 17. |
- Andersson, Nina, et al.
(författare)
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Lymphocyte antigen 6 superfamily member D is a marker of urothelial and squamous differentiation : Implications for risk stratification of bladder cancer
- 2020
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Ingår i: Biomarker research. - : Springer Science and Business Media LLC. - 2050-7771. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Screening across a multitude of normal and malignant tissues revealed an enhanced expression of lymphocyte antigen 6 superfamily member D (LY6D) in squamous epithelium and urothelium, as well as in malignancies derived therefrom. The aim of this study was to further delineate the protein expression of LY6D in urothelial bladder cancer, with particular attention to its relationship with clinicopathological characteristics and patient outcome. Methods: Immunohistochemical expression of LY6D was assessed in tissue microarrays with urothelial bladder cancer tumours from three independent patient cohorts; one with transurethral resection of the bladder (TURB) specimens of mixed tumour stages from 110 consecutive cases, one with tumours of mixed stages from 260 incident cases in a population-based cohort, and one with paired TURB specimens, resected tumours and a subset of lymph node metastases from 145 patients with muscle-invasive bladder cancer (MIBC). Chi-square and non-parametric tests were applied to examine associations of LY6D expression with clinicopathological characteristics. Kaplan-Meier and Cox regression analyses were applied to examine 5-year overall survival (OS) and recurrence free survival (RFS) in relation to LY6D expression. Results: In the two cohorts with mixed stages, positive LY6D expression was denoted in 63 and 64% of the cases, respectively, and found to be significantly higher in low-grade and less invasive tumours. Negative LY6D expression was significantly associated with a reduced 5-year OS, although not independently of established prognostic factors. In the population-based cohort, LY6D expression was higher in tumours with squamous differentiation and lower in other variant histologies compared to pure urothelial tumours, and the association of LY6D expression with survival was somewhat enhanced after exclusion of the former. LY6D expression was generally lower in the MIBC cohort, and even more reduced in resected tumours compared to TURB specimens in patients who had not received neoadjuvant chemotherapy. There were no significant associations between LY6D expression and RFS, neither allover nor in relation to neoadjuvant chemotherapy. Conclusion: LY6D is a marker of urothelial and squamous differentiation that may add useful diagnostic and prognostic information to better guide the clinical management of bladder cancer, given that the presence of variant histology is taken into account.
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| 18. |
- Andersson, Niklas, et al.
(författare)
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Patient experiences of being cared for by nursing students in a psychiatric education unit
- 2020
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Ingår i: Nordic journal of nursing research. - : Sage Publications. - 2057-1585 .- 2057-1593. ; 40:3, s. 142-150
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Tidskriftsartikel (refereegranskat)abstract
- Patients are vital for student learning. However, research has primarily focused on student nurse learning from a student or supervisor perspective; few studies have investigated patient perspectives. This study examines student care practice for patients in acute psychiatric day care. The aim was to describe patients’ experiences of care by student nurses in a psychiatric education unit, a collaboration between the clinic and academia. Data were collected through 17 lifeworld interviews with patients, of which 10 also included observations. Data have been analysed for meanings using reflective, lifeworld research (RLR). The findings reveal that the encounters involve an interactive process of giving and receiving, providing students with both health opportunities and risks. The findings can further be described by the following constituents: exposed and vulnerable; responsibility to support; the importance of accessibility; reciprocity; and engagement that evokes the desire to live a life with dignity. In a patient–student community, there are prerequisites for proper caring. Patient health seems to be positively affected when patients are involved in both their own care and student learning.
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| 19. |
- Antonsen, Simen, et al.
(författare)
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Atmospheric Chemistry of tert-butylamine and AMP
- 2017
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Ingår i: Energy Procedia. Volume 114, 2017, Pages 1026-103213th International Conference on Greenhouse Gas Control Technologies, GHGT 2016; Lausanne; Switzerland; 14 November 2016 through 18 November 2016. - : Elsevier BV. - 1876-6102.
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Konferensbidrag (refereegranskat)abstract
- © 2017 The Authors. The atmospheric chemistry of (CH 3 ) 3 CNH 2 (tert-butylamine, tBA) and (CH 3 ) 2 (CH 2 OH)CNH 2 (2-amino-2-methyl-1-propanol, AMP) has been studied by quantum chemistry methods and in photo-oxidation experiments in the EUPHORE chamber in Valencia (Spain). Aerosol formation and composition has been quantified. Yields of nitramines and other products in the photo-oxidations have been determined and complete photo-oxidation schemes including branching between the major reaction routes have been obtained. Published by Elsevier Ltd.
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| 20. |
- Antonsen, Stian, et al.
(författare)
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Structures for collaboration and networked adaptation : Emerging themes from the COVID-19 pandemic
- 2023
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Ingår i: Journal of Emergency Management. - : Weston Medical Publishing. - 1543-5865. ; 21:7, s. 71-84
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Tidskriftsartikel (refereegranskat)abstract
- The COVID-19 pandemic is a crisis that is "creeping" in its onset and "slow-burning" in its duration. It is characterized by extreme uncertainty, ambiguity, and complexity, presenting an unprecedented need for response across sectors and political-administrative levels. While there has been an explosion of research papers into the national strategies for handling the pandemic, empirical publications on the local and regional management are still scarce. This paper presents early empirical insights into key collaborative functions in Norway and Sweden, with an ambition to contribute to a research agenda focusing on the collaborative practices of pandemic crisis management. Our findings point to a set of themes that are all related to emerging collaborative structures, that fill holes in pre-established structures for dealing with crises, and that have been important for being able to effectively deal with the pandemic. At the municipal and regional levels, we see more examples of well-adapted collaborative practices than we see the wickedness of the problem causing inertia and paralysis. However, the emergence of new structures indicates a need to adapt organizational structures to the existing problem, and the duration of the current crisis allows for significant evolution of collaborative structures within the various phases of the pandemic. The lessons that can be drawn from this reveal a need for reconsideration of some of the basic assumptions of crisis research and practice, in particular the so-called similarity principle that is a cornerstone of emergency preparedness organization in many countries, including Norway and Sweden.
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