| 671. |
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| 672. |
- Johansson, Kristina, et al.
(författare)
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Multicomponent Hf-Nb-Ti-V-Zr nitride coatings by reactive magnetron sputter deposition
- 2018
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Ingår i: Surface & Coatings Technology. - : ELSEVIER SCIENCE SA. - 0257-8972 .- 1879-3347. ; 349, s. 529-539
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Tidskriftsartikel (refereegranskat)abstract
- Multicomponent nitride coatings of the Hf-Nb-Ti-V-Zr system with different Hf content (0-18 at.%) were deposited using reactive dc magnetron sputtering. Coatings with lower Hf content (0-7 at.%) were found to consist of a single solid solution phase with NaCl-type structure (space group Fm-3m). Coatings with higher Hf content (10-18 at.%) showed a two-phase material consisting of cubic Fm-3m and tetragonal I4/m:run solid solution phase. The lattice distortion, estimated by calculating the delta-parameter under the assumption of a single solid solution phase, varied between 3.8 and 4.0% and slightly decreased with increasing Hf content. SEM and TEM cross section images showed a columnar microstructure with columns that were frayed on the surface or throughout the whole column. The column size decreased as Hf content increased. The hardness increased from 8 to 19 GPa with increased Hf content, which most probably is related to the change in microstructure rather than change in lattice distortion. The electrical resistivity for all samples ranged between 231 and 286 mu Omega cm.
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| 673. |
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| 674. |
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| 675. |
- Johansson, Leif B. G., et al.
(författare)
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An azide functionalized oligothiophene ligand - A versatile tool for multimodal detection of disease associated protein aggregates
- 2015
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 63, s. 204-211
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Tidskriftsartikel (refereegranskat)abstract
- Ligands for identifying protein aggregates are of great interest as such deposits are the pathological hallmark of a wide range of severe diseases including Alzheimers and Parkinsons disease. Here we report the synthesis of an azide functionalized fluorescent pentameric oligothiophene that can be utilized as a ligand for multimodal detection of disease-associated protein aggregates. The azide functionalization allows for attachment of the ligand to a surface by conventional click chemistry without disturbing selective interaction with protein aggregates and the oligothiophene-aggregate interaction can be detected by fluorescence or surface plasmon resonance. In addition, a methodology where the oligothiophene ligand is employed as a capturing molecule selective for aggregated proteins in combination with an antibody detecting a distinct peptide/protein is also presented. We foresee that this methodology will offer the possibility to create a variety of multiplex sensing systems for sensitive and selective detection of protein aggregates, the pathological hallmarks of several neurodegenerative diseases.
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| 676. |
- Johansson, Maria A, et al.
(författare)
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Early-Life Gut Bacteria Associate with IL-4-, IL-10- and IFN-γ Production at Two Years of Age
- 2012
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:11, s. e49315-(9 pp)
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Tidskriftsartikel (refereegranskat)abstract
- Microbial exposure early in life influences immune maturation and potentially also the development of immune-mediated disease. Here we studied early-life gut colonization in relation to cytokine responses at two years of age. Fecal samples were collected from infants during the first two months of life. DNA was extracted from the fecal samples and Bifidobacterium (B.) adolescentis, B. breve, B. bifidum, a group of lactobacilli (L. casei, L. paracasei and L. rhamnosus) as well as Staphylococcus (S.) aureus were detected with real time PCR. Peripheral mononuclear cells were stimulated with phytohaemagglutinin (PHA) and numbers of IL-4-, IL-10- and IFN-γ secreting cells were evaluated using ELISpot. We further stimulated peripheral blood mononuclear cells with bacterial supernatants in vitro and assessed the IL-4-, IL-10- and IFN-γ inducing capacity by flow cytometry and ELISA. Early S. aureus colonization associated with higher numbers of IL-4- (p = 0.022) and IL-10 (p = 0.016) producing cells at two years of age. In contrast to colonization with S. aureus alone, co-colonization with lactobacilli associated with suppression of IL-4- (p = 0.004), IL-10- (p = 0.004) and IFN-γ (p = 0.034) secreting cells. In vitro stimulations of mononuclear cells with bacterial supernatants supported a suppressive role of L. rhamnosus GG on S. aureus-induced cytokine responses. We demonstrate that the early gut colonization pattern associates with the PHA-induced cytokine profile at two years of age and our in vitro findings support that specific bacterial species influence the T helper cell subsets. This suggests that dysbiosis in the early microbiota may modulate the risk of developing inflammatory conditions like allergy.
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| 677. |
- Johansson, Mikael, et al.
(författare)
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A novel depleted semi-insulating silicon material for high frequency applications
- 2002
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Ingår i: Progress in SOI Structures and Devices Operating at Extreme Conditions. Proceedings of the NATO Advanced Research Workshop. ; , s. 333-
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Konferensbidrag (refereegranskat)abstract
- Wafer bonding has been used to manufacture a silicon material intended as substrate for high frequency applications. The space charge regions surrounding the bonded silicon/silicon interface deplete the silicon thereby causing semiinsulating behaviour at high frequencies. The material has been characterized electrically for frequencies up to 40 GHz using metal transmission lines and crosstalk structures on its surface. Measurements on the depleted silicon/silicon structures has been compared to similar measurements on bulk silicon wafers of different resistivities, SIMOX wafers and quartz. The results show that the material has potential to be used at high frequencies
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| 678. |
- Johansson, Maria, et al.
(författare)
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Assessing cultural ecosystem services as individuals’ place-based appraisals
- 2019
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Ingår i: Urban Forestry & Urban Greening. - München : Elsevier. - 1618-8667 .- 1610-8167. ; 39, s. 79-88
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Tidskriftsartikel (refereegranskat)abstract
- Though the existence of cultural ecosystem services is dependent on people’s activities and experiences, these services are still commonly assessed using top–down approaches. In this study, appraisal theory and research into human responses to natural environments formed the basis of a systematic multilevel investigation of appraisals of created wetland areas. The aim was to explore how appraisals could be used as a bottom–up approach to assessing the perceived contribution of wetland areas s to people’s quality of life (QoL). In total, 111 participants assessed environmental perceptions, affective experiences, and restorative potentials on site at three wetlands in Sweden. The results indicate that wetland areas and specific places with different ecological functions within these areas were appraised significantly differently. Moreover these places were perceived to support various QoL aspects to different degrees. Concepts and methods tested here give environmental planners hands-on tools for facilitating communication with expected users about the cultural ecosystem services of created wetland areas. © 2019
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| 679. |
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| 680. |
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