| 1. |
- Nordberg Karlsson, Eva, et al.
(författare)
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Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus
- 1998
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Ingår i: FEMS Microbiology Letters. - 0378-1097. ; 168:1, s. 1-7
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Tidskriftsartikel (refereegranskat)abstract
- The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose binding domain of CenC from Cellulomonas fimi, we suggest that the binding is mediated by the two N-terminally repeated domains. Copyright (C) 1998 Federation of European Microbiological Societies.
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| 2. |
- Schembri, Mark A., et al.
(författare)
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Linker insertion analysis of the FimH adhesin of type 1 fimbriae in an Escherichia coli fimH-null background
- 1996
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Ingår i: FEMS Microbiology Letters. - 0378-1097. ; 137:2-3, s. 257-263
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Tidskriftsartikel (refereegranskat)abstract
- The gene encoding the Escherichia coli FimH adhesin of type 1 fimbriae has been subjected to linker insertion mutagenesis. Amino acid changes were introduced at a number of positions spanning the entire sequence in order to probe the structure-function relationship of the FimH protein. The effect of these mutations on the ability of bacteria to express a D-mannose binding phenotype was assessed in a fimH null mutant (MS4) constructed by allelic exchange in the. E coli K-12 strain PC31. Mutations mapping at amino acid residues 36, 58 and 279 of the mature FimH protein were shown to completely abolish binding to D-mannose receptors. Differences in the level of fimbriation were also observed as a result of some of the mutations in the fimH gene. These mutants may prove useful in dissecting receptor-ligand interactions by defining regions of the FimH protein that are important in erythrocyte binding.
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| 3. |
- Tunlid, A., et al.
(författare)
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Transformation of the nematode-trapping fungus Arthrobotrys oligospora
- 1999
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Ingår i: FEMS Microbiology Letters. - 0378-1097. ; 173:1, s. 111-116
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Tidskriftsartikel (refereegranskat)abstract
- The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%. Copyright (C) 1999 Federation of European Microbiological Societies.
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| 4. |
- Wouters, Johanna, et al.
(författare)
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Cloning and expression of a putative cyclodextrine glycosyltransferase from the symbiotically competet cyanobacteruium Nostoc sp. PCC 9229
- 2003
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Ingår i: FEMS Microbioloy Letters. - 0378-1097. ; 219:2, s. 181-185
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Tidskriftsartikel (refereegranskat)abstract
- A polymerase chain reaction-based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis. The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt. Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free-living nitrogen-fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium.
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| 5. |
- Asiegbu, F.O., et al.
(författare)
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Isolation of a novel antimicrobal peptide gene (Sp-AMP) homologue from Pinus sylvestris (Scots pine) following infection with the root rot fungus Heterobasidion annosum
- 2003
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 228:1, s. 27-31
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Tidskriftsartikel (refereegranskat)abstract
- A new family of antimicrobial peptide homologues termed Sp-Amp has been discovered in Pinus sylvestris (Scots pine). This is the first report of such proteins to be characterized in a conifer species. Sp-AMP1 was identified in a substructured cDNA library of root tissue infected with the root rot fungus Heterobasidion annosum and encodes a mature peptide of 79 amino acid residues. Three additional members of the Sp-AMP family (Sp-AMPs 2–4) encode cysteine-rich proteins of 105 amino acids, each containing an N-terminal region with a probable cleavage signal sequence. Northern analysis confirmed that Sp-AMP expression is elevated in Scots pine roots upon infection with H. annosum. These peptides share 64% amino acid identity with a mature protein from Macadamia integrifolia (MiAMP1), which allowed us to build a homology model for preliminary analysis. Southern analyses further confirmed that several copies of the gene are present in the Scots pine genome. The potential significance of Sp-AMP in the H. annosum–conifer pathosystem is discussed.
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| 6. |
- Daeschel, M.A., et al.
(författare)
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Microbial ecology of fermenting plant materials
- 1987
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 46:3, s. 357-367
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Tidskriftsartikel (refereegranskat)abstract
- The lactic acid fermentation of plant materials is presented from an ecological perspective emphasizing microbial interactions and their influence on the production of fermented plant foods and silage. The plant lactic acid bacteria are discussed in terms of evolution; epiphytic function; physical distribution within fermented material; substrates and products; microbial sequences in fermentation; interactions among species; pure culture fermentation; and starter culture development. © 1987.
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| 7. |
- Grahn, Niclas, et al.
(författare)
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Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons
- 2003
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 219:1, s. 87-91
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Tidskriftsartikel (refereegranskat)abstract
- Using a sensitive and rapid method combining broad-range PCR amplification of bacterial 16S rDNA fragments and pyrosequencing for detection, identification and typing, we have found contaminating bacterial DNA in our reagents used for PCR. Identified bacteria are the water-borne bacterial genera Pseudomonas, Stenotrophomonas, Xanthomonas, Ralstonia and Bacillus. Our results are in concordance with recent reports of contaminated industrial water systems. In light of this conclusion, we believe that there is a need for increased awareness of possible contamination in uncertified widely used molecular biology reagents, including ultra-pure water. Since sequence-based 16S rDNA techniques are used in a variety of settings for bacterial typing and the characterization of microbial communities, we feel that future certification of molecular biology reagents, as free of nucleic acids, would be advantageous. ⌐ 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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| 8. |
- Lai, Xin-He, et al.
(författare)
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Francisella strains express hemolysins of distinct characteristics
- 2003
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Ingår i: FEMS Microbiology Letters. - : Elsevier. - 0378-1097 .- 1574-6968. ; 224:1, s. 91-95
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Tidskriftsartikel (refereegranskat)abstract
- Historically, Francisella strains have been described as nonhemolytic. In this study, we show by use of solid and liquid hemolysis assays that some Francisella strains have hemolytic properties. The Francisella novicida type strain U112 is hemolytic to horse erythrocytes and Francisella philomiragia type strain FSC144 is hemolytic towards both human and horse erythrocytes. The F. novicida strain U112 released a protein (novilysin A) into the culture supernatant which cross-reacted with antiserum against Escherichia coli HlyA whereas there was no similar protein detectable with this cross-reactive property from the supernatant of the F. philomiragia strain.
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| 9. |
- Lehtio, J., et al.
(författare)
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Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain
- 2001
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 195:2, s. 197-204
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Tidskriftsartikel (refereegranskat)abstract
- The immobilization of recombinant staphylococci onto cellulose fibers through surface display of a fungal cellulose-binding domain (CBD) was investigated. Chimeric proteins containing the CBD from Trichoderma reesei cellulase Cel6A were found to be correctly targeted to the cell wall of Staphylococcus carnosus cells. since full-length proteins could be extracted and affinity-purified. Furthermore. surface accessibility of the CBD was verified using a monoclonal antibody and functionality in terms of cellulose-binding was demonstrated in two different assays in which recombinant staphylococci were found to efficiently bind to cotton fibers. The implications of this strategy of directed immobilization Tor the generation of whole-cell microbial tools Fur different applications will be discussed.
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| 10. |
- Leitz, G, et al.
(författare)
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Laser-based micromanipulation for separation and identification of individual Frankia vesicles
- 2003
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 224:1, s. 97-100
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Tidskriftsartikel (refereegranskat)abstract
- In studies of symbiotic efficiency it is of great importance to identify and separate individual Frankia strains from a nodule. Therefore, a new laser-based micromanipulation technique has been developed in which individual vesicles from root nodules of two Frankia-Alnus symbioses have been successfully cut loose and separated from clusters of vesicles in sterile conditions under light microscopy using a laser scalpel and optical tweezers. Vesicles from the Alnus incana-Frankia AvCIl symbiosis were successfully isolated and grown in culture using this technique. The DNA from both Frankia sources was amplified by polymerase chain reaction (PCR). The work shows that a combination of laser-based manipulation techniques and PCR can be used for the separation and study of individual vesicles. This novel laser-based micromanipulation technique opens up various new possibilities, for instance, to study whether several Frankia strains can grow simultaneously in the same root nodule. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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