| 1. |
- Barkarmo, Sargon, et al.
(författare)
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Biofilm formation on polyetheretherketone and titanium surfaces
- 2019
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Ingår i: Clinical and Experimental Dental Research. - : Wiley. - 2057-4347. ; 5:4, s. 427-437
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Polyetheretherketone (PEEK) is a polymer used in devices in orthopedic and dental rehabilitation. The aim of this in vitro study was to compare biofilm formation by a range of important oral bacterial species on PEEK, blasted PEEK, commercially pure titanium (cp-Ti), and titanium-6 aluminium-4 vanadium (Ti6Al4V). Material and methods: Coin-shaped samples were manufactured, and the surfaces were characterized using optical interferometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and contact angle measurements. Bacterial species of Streptococcus sanguinis, Streptococcus oralis, Enterococcus faecalis, and Streptococcus gordonii were cultured on the four material surfaces for varying amounts of time. Biofilms were quantified following staining with crystal violet. Results: Roughness and contact angle results showed blasted PEEK>PEEK>cp-Ti=Ti6Al4V. There was increased biofilm formation on blasted PEEK by S. sanguinis, S. oralis, and S. gordonii, whereas the bacterial adhesion was similar on PEEK, cp-Ti, and Ti6Al4V. The bacterial growth of E. faecalis was significantly higher on cp-Ti compared with the other three groups. Conclusion: The results, taking into consideration the biofilm formation, suggest that PEEK should perform as well as cp-Ti or TiAl6V4 when used as a dental restorative material. © 2019 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
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| 2. |
- Barkarmo, Sargon, et al.
(författare)
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Enhanced bone healing around nanohydroxyapatite-coated polyetheretherketone implants : An experimental study in rabbit bone
- 2014
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Ingår i: Journal of biomaterials applications. - : Sage Publications. - 0885-3282 .- 1530-8022. ; 29:5, s. 737-747
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate the bone response to threaded polyetheretherketone (PEEK) implants coated with nanohydroxyapatite. Materials and methods: A total of 39 PEEK implants were coated with nanocrystalline hydroxyapatite and 39 uncoated implants were used as controls. The implant surface was characterized by optical interferometry and scanning electron microscope. The implants were inserted in the tibia and femur of 13 rabbits. After 6 weeks of healing, quantitative and qualitative analyses were performed. Results: The test implants showed significantly higher removal torque test values compared with the control group. Histomorphometric evaluation demonstrated higher bone-to-implant contact for the test implants; however, there were no differences in bone area between the groups. Qualitative histological analyses demonstrated inflammatory cellular reactions in close vicinity of both implant surfaces. A two-cell layer of foreign body giant cells was observed irrespective of sample type. Conclusion: Our findings demonstrate that implants with a threaded design render good stability to PEEK in both coated and uncoated implants. Nanohydroxyapatite-coated PEEK implants demonstrated improved bone formation compared with uncoated controls.
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| 3. |
- Barkarmo, Sargon
(författare)
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In vivo and in vitro studies of polyetheretherketone: bone formation, inflammatory response and biofilm formation
- 2019
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In prosthetic dentistry, restorative materials are used to replace missing teeth and tissues, so as to maintain oral functionalities and comfort for the patient. Depending on the clinical problem, metals, ceramics or polymers, are used both in dentistry and orthopedics. The present thesis focuses on the polymer material polyetheretherketone (PEEK), which has been used in orthopedic applications for about 30 years, mainly as a component of spinal devices - as such it has provided good clinical outcomes. PEEK has recently been adopted as part of dental rehabilitation owing to its many favorable properties, including high-level mechanical strength, chemical resistance, and biocompatibility. Therefore, it is of interest and important to extend our basic understanding of PEEK as a material that can be used in various prosthetic devices. Moreover, it is important to investigate whether modifications made to the surface of the material generate outcomes that may be translated to prosthetic dentistry, thereby using PEEK as a more broadly applicable dental material. The overall aim of this thesis was to use in vivo and in vitro experimental methods to investigate the potential of PEEK as a material for use in dental devices. In vivo studies were conducted to investigate the host bone responses to cylindrically shaped and threaded PEEK implants that were coated with nanocrystalline hydroxyapatite (nanoHA), as compared to uncoated control implants. The results revealed significantly higher mean values for the biomechanical and histomorphometric parameters for the nanoHA PEEK, as compared to the control material. The levels of cytokines expressed by peripheral blood mononuclear cells (PBMCs) when exposed in vitro to PEEK, blasted PEEK, and titanium 6-aluminum 4-vanadium (Ti6Al4V) were investigated at different time-points. The PBMCs produced significantly higher levels of pro-inflammatory cytokines when exposed to the PEEK surface than when exposed to the Ti6Al4V surface. The blasted PEEK surface induced the highest level of proinflammatory cytokine release from the PBMCs. The ability to form a biofilm in vitro was assessed by inoculating oral bacterial species onto PEEK, blasted PEEK, commercially pure titanium (cp-Ti), and Ti6Al4V. Biofilm formation was quantified after staining with crystal violet. The blasted PEEK showed increased biofilm formation by S. sanguinis, S. oralis and S. gordonii as compared to the other surfaces, while the levels of bacterial adhesion to PEEK, cp-Ti, and Ti6Al4V were similar. It appears that nanoHa-coated, threaded PEEK implants improve bone formation, as compared to uncoated PEEK implants, and that PEEK induces a stronger inflammatory response than does Ti6Al4V. The biofilm formation results suggest that the level of bacterial adhesion to PEEK is similar to that of cp-Ti and Ti6AlV4. Within the limitations of the methods used in the present thesis, it can be concluded that PEEK may have potential as a material for use in various dental applications.
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| 4. |
- Barkarmo, Sargon, et al.
(författare)
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Inflammatory cytokine release from human peripheral blood mononuclear cells exposed to polyetheretherketone and titanium-6 aluminum-4 vanadium in vitro.
- 2018
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Ingår i: Journal of biomaterials applications. - : SAGE Publications. - 1530-8022 .- 0885-3282. ; 33:2, s. 245-258
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Tidskriftsartikel (refereegranskat)abstract
- Objective To investigate the cytokine expression profiles of blood cells exposed to polyetheretherketone and titanium-6 aluminum-4 vanadium materials in vitro. Materials and methods Coin-shaped samples composed of titanium-6 aluminum-4 vanadium, polyetheretherketone, and blasted polyetheretherketone were manufactured. The surfaces of the coins were characterized using optical interferometry, scanning electron microscopy, and contact angle measurements. Peripheral blood mononuclear cells collected from 10 blood donors were cultured for one, three, and six days in the presence or absence of the coins, and then assayed for cytokine production. Quantification of the peripheral blood mononuclear cells attached to the coins was performed using confocal microscopy after immunofluorescence staining. Results The machined titanium-6 aluminum-4 vanadium coins had a smoother surface topography compared to the machined polyetheretherketone and blasted polyetheretherketone. The highest mean contact angle was noted for the blasted polyetheretherketone, followed by the machined polyetheretherketone and titanium-6 aluminum-4 vanadium. The peripheral blood mononuclear cells produced significantly more proinflammatory cytokines when exposed to the polyetheretherketone surface compared to the titanium-6 aluminum-4 vanadium surface, while the blasted polyetheretherketone induced the highest level of proinflammatory cytokine release from the peripheral blood mononuclear cells. Significantly more cells attached to both polyetheretherketone surfaces, as compared to the titanium-6 aluminum-4 vanadium surface. Conclusion Polyetheretherketone induces a stronger inflammatory response from peripheral blood mononuclear cells than does titanium-6 aluminum-4 vanadium. Surface topography has an impact on cytokine release from peripheral blood mononuclear cells.
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| 5. |
- Barkarmo, Sargon, et al.
(författare)
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Nano-hydroxyapatite-coated PEEK implants : a pilot study in rabbit bone
- 2013
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Ingår i: Journal of Biomedical Materials Research. Part A. - : John Wiley & Sons. - 1549-3296 .- 1552-4965. ; 101A:2, s. 465-471
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Tidskriftsartikel (refereegranskat)abstract
- Osseointegration of surface-modified polyetheretherketone (PEEK) implants was studied in vivo. A total of 18 cylinder-shaped PEEK implants were inserted in the femurs of nine New Zealand rabbits; half were coated with nanocrystalline hydroxyapatite (nanoHA) and half were uncoated controls. Healing time was 6 weeks. Samples were retrieved with the implant and surrounding tissue, processed to cut and ground sections, and analyzed histomorphometrically. The implant surfaces were analyzed with optical interferometry, scanning electron microscopy (SEM), atomic force microscopy, and X-ray photoelectron spectroscopy (XPS). NanoHA-coated PEEK surfaces had lower height deviation (Sa) than controls [mean ± SD: 0.41 μm (± 0.14) vs. 0.96 μm (± 0.28)]. SEM images showed the nanoHA crystals as a thin layer on the polymer surface. XPS analysis of the coated implants showed a Ca/P ratio of 1.67. Histomorphometry indicated that the nanoHA-coated implants had more bone-to-implant contact [16% (± 4.7) vs. 13% (± 9.3)] and more bone area [52% (± 9.5) vs. 45% (± 11.9)]. We found no difference between smooth nanoHA-coated cylinder-shaped PEEK implants and uncoated controls. However, higher mean bone-to-implant contact indicated better osseointegration in the coated implants than in the uncoated controls. The large number of lost implants was interpreted as a lack of primary stability due to implant design.
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| 6. |
- Gottlow, Jan, 1951, et al.
(författare)
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An experimental comparison of two different clinically used implant designs and surfaces.
- 2012
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Ingår i: Clinical implant dentistry and related research. - : Wiley. - 1708-8208 .- 1523-0899. ; 14 Suppl 1
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Tidskriftsartikel (refereegranskat)abstract
- Various designs of dental implants representing different geometries and surface technologies are commercially available and clinically used in patients. However, data with regard to bone tissue responses and stability for comparison of their biologic performances are rare.
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| 7. |
- Harada, A., et al.
(författare)
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Mechanical and microstructural properties of ultra-translucent dental zirconia ceramic stabilized with 5 mol% yttria
- 2020
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Ingår i: Journal of the Mechanical Behavior of Biomedical Materials. - : Elsevier BV. - 1751-6161. ; 111
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Tidskriftsartikel (refereegranskat)abstract
- Monolithic dental prostheses fabricated from 3 mol.% yttria-stabilized zirconia (3YZ) are becoming increasingly popular. Recently, 5 mol.% yttria-stabilized zirconia (5YZ) which significantly improves the translucency of 3YZ has been prepared. However, its mechanical and microstructural properties, especially those affected by low-temperature degradation (LTD), have not been fully elucidated yet. The objective of the present study was to establish the relationship between the flexural strength of 5YZ with or without autoclave-induced LTD and its microstructural properties. For this purpose, a total of 320 bar-shaped specimens were cut from 5YZ and 3YZ blocks, and half of the specimens in each group were autoclaved at 134 °C for 50 h. Their flexural strengths were determined by conducting three-point bending tests, and the obtained results were analyzed by the Weibull statistical method. Grain sizes and crystalline structures of the specimens were analyzed by scanning electron microscopy (SEM) and X-ray diffraction, respectively. Additionally, the LTD-induced phase transformation was examined by Raman microscopy and cross-sectional surface analysis. The characteristic strengths of 5YZ and 3YZ were approximately 620 and 950 MPa, respectively, and 5YZ was found to be more resistant to LTD in terms of phase transformation than 3YZ. However, a low amount of the monoclinic phase was detected even in 5YZ after 50 h of autoclaving, which significantly decreased its flexural strength and reliability. The results of SEM analysis revealed that 5YZ was composed of two distinct regions: a dominant matrix with large grains (median size: 0.8 μm) and scattered areas with small grains (median size: 0.4 μm). Phase transformation analysis and fractography data indicated that the small-grain region was strongly affected by LTD and likely represented a fracture origin. The described properties should be considered during the clinical application of monolithic 5YZ dental prostheses. © 2020 Elsevier Ltd
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| 8. |
- Johansson, Pär, et al.
(författare)
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Biomechanical, histological, and computed X-ray tomographic analyses of hydroxyapatite coated PEEK implants in an extended healing model in rabbit
- 2018
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Ingår i: Journal of Biomedical Materials Research Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 106:5, s. 1440-1447
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Tidskriftsartikel (refereegranskat)abstract
- A nanosized hydroxyapatite (HA) modification on polyetheretherketone (PEEK) using a novel spin coating technique was investigated in a rabbit model. Spin coating technique creates a 20-40 nm thick layer of nanosized HA particles with similar shape, size, and crystallinity as human bone. Some implants were designed with a perforating hole in the apical region to mimic a fusion chamber of a spinal implant. The coating nano-structures were assessed using a scanning electron microscope. The in vivo response to HA-PEEK was compared to untreated PEEK with respect to removal torque, histomorphometry, and computed microtomography. The HA-coated and pure PEEK implants were inserted in the tibia and femur bone according to simple randomization. The rabbits were sacrificed 20 weeks after implantation. Removal torque analysis showed significantly higher values for HA-PEEK. Qualitative histological evaluation revealed an intimate contact between PEEK and the bone at the threads and perforated hole. Histomorphometric assessment showed higher bone-implant and bone area values for HA-PEEK but without statistical significance. The effect of the HA coating showed most prominent effect in the removal torque which may be correlated to an alteration in the bone quality around the HA-PEEK implants. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1440-1447, 2018.
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| 9. |
- Nakamura, K., et al.
(författare)
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Influence of crystalline phase transformation induced by airborne-particle abrasion and low-temperature degradation on mechanical properties of dental zirconia ceramics stabilized with over 5 mol% yttria
- 2022
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Ingår i: Journal of the Mechanical Behavior of Biomedical Materials. - : Elsevier BV. - 1751-6161. ; 125
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Tidskriftsartikel (refereegranskat)abstract
- Monolithic dental prostheses fabricated from 5 mol% yttria-stabilized zirconia (5YZ) have been developed to improve the translucency of conventional 3 mol% yttria-stabilized zirconia. In this study, we aimed to evaluate the influence of airborne-particle abrasion (APA) and low-temperature degradation (LTD) on the mechanical properties of 5YZ in association with the crystalline phase transformation. In total, 120 disc-shaped specimens of two brands of 5YZ (Lava Esthetic and Katana UTML) were prepared. The specimens were divided into four groups (n = 15 for each group): (i) control, (ii) APA, (iii) LTD, and (iv) APA + LTD groups. APA was performed with 50 mu m alumina particles, and LTD was induced by autoclaving at 134 degrees C for 50 h. The biaxial flexural strength of the specimens was assessed using a piston-on-three-ball test according to ISO 6872:2015, and Vickers hardness was determined using a microhardness tester. The crystalline phase was analyzed by the Rietveld refinement of X-ray diffraction patterns. APA significantly increased the flexural strength of the Lava Esthetic specimens, whereas LTD hardly affected the strength of both materials. APA and APA + LTD significantly increased the Vickers hardness of both materials. According to Rietveld analysis, the pseudocubic phase was predominant in both materials, i.e., 66 mass% and 81 mass% in the Lava Esthetic and Katana UTML specimens, respectively. APA induced the rhombohedral phase at approximately 37 mass% in both materials, while LTD induced the monoclinic phase at 2.8 mass% in the Lava Esthetic specimens and 0.9 mass% in the Katana UTML specimens. APA + LTD weakly affected the amount of the rhombohedral phase but slightly increased the amount of the monoclinic phase. These findings suggest that APA may improve the mechanical properties of 5YZ, particularly hardness, via the generation of the rhombohedral phase. In contrast, the influence of LTD on the mechanical and microstructural properties of 5YZ was limited.
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| 10. |
- Olander, Julia, 1984, et al.
(författare)
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Inflammatory Gene Profile and Particle Presence in Peri-Implant Mucosa: a Pilot Study on 9 Patients
- 2023
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Ingår i: Jomr-Journal of Oral & Maxillofacial Research. - 2029-283X. ; 14:3
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: The purpose of this pilot study is to compare gene expression in mucosa around dental implants with zirconia abutment to titanium and investigate presence of particles in mucosa samples and on implant heads. Material and Methods: Ten patients with a single implant supported prosthesis connected to zirconia or titanium abutments were invited at the five-year control. A clinical examination and a survey on experience of function and appearance were conducted. A mucosa biopsy taken in close vicinity to the implant were analysed by real-time polymerase chain reaction (qPCR) and presence of particles in a scanning electron microscope/energy-dispersive X-ray spectroscope (SEM/EDX). Cytological smear samples were collected and analysed through inductively coupled plasma mass spectrometry (ICP-MS) to investigate presence of particles on implant heads. Results: In total, 9 patients participated in the study, five with titanium abutments and four with zirconia abutments. All patients were satisfied with function and aesthetics. Titanium and iron particles were detected in mucosa biopsies. The ICP -MS analysis demonstrated presence of zirconia and titanium. Several proinflammatory genes were upregulated in the zirconia abutment group. Conclusions: Around zirconia abutments a slight increase in proinflammatory response and amount of wear particles was seen as compared to titanium. Wear particles of titanium were present in all soft tissue samples, however zirconia particles only in the samples from implants heads/mucosa with zirconia abutments.
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