| 1. |
- Malmberg, Leona, 1973-, et al.
(författare)
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Endodontic follow-up practices, sources of knowledge, and self-assessed treatment outcome among general dental practitioners in Sweden and Norway
- 2020
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Ingår i: Acta Odontologica Scandinavica. - : Informa UK Limited. - 0001-6357 .- 1502-3850. ; 78:7, s. 547-552
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To reduce the gap between what can be achieved in endodontic treatments and the observed treatment outcome among general dental practitioners, the present study set out to assess the status of the endodontic practices as regards to knowledge and self-assessed skills among general dental practitioners in Sweden and Norway. Material and method: The questionnaire was sent to 1384 general dental practitioners. It contained questions regarding access to continuing education in endodontics, sources of knowledge for clinical management of patients, post-operative follow-ups, self-assessed success-rate, and the initial diagnosis impact on the outcome of endodontic treatments. Results: The response rate was 61.4%. Almost half estimated their endodontic success-rate to be 90%. About two-thirds of the respondents did not know, or did not believe, that the initial diagnosis could affect the outcome of their endodontic treatments. Respondents who did not believe the diagnosis could impact the outcome were more likely to estimate their success rate as the highest (p<.001). Less than half performed post-operative follow-ups a year after treatment. A third of the respondents had not attended any continuing endodontic education. Conclusion: Dentists who do not receive regular feedback on their treatments may lack insight into their own shortcomings. If this is combined with insufficient knowledge and understanding it may result in sub-par endodontic treatments being performed. It is important to have reliable ways to communicate current endodontic knowledge and to establish robust methods that may help dentists accurately assess their own performance in endodontics. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Acta Odontologica Scandinavica Society.
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| 2. |
- Malmberg, Leona, et al.
(författare)
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Endodontic infection control routines among general dental practitioners in Sweden and Norway: a questionnaire survey
- 2019
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Ingår i: Acta Odontologica Scandinavica. - : Informa UK Limited. - 0001-6357 .- 1502-3850. ; 77:6, s. 434-438
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Tidskriftsartikel (refereegranskat)abstract
- Objective: The purpose of this study was to investigate endodontic infection prevention and control routines among general dental practitioners in Sweden and Norway.Materials and methods: A questionnaire was sent by email to 1384 general dental practitioners employed in Sweden and Norway. The participants were asked questions concerning different aspects of infection prevention and control during endodontic treatment; use of rubber dam, sealing of rubber dam, antibacterial solutions, and use of hand disinfectant and gloves.Results: The response rate was 61.4% (n: 819). 96.9% reported routinely using rubber dam during endodontic treatment. 88.3% reported always, or sometimes, sealing the area between rubber dam and tooth. Most disinfected the endodontic operative field, but the antibacterial solutions used varied. 11.9% did not use gloves at all during treatment, and 10.5% did not use hand disinfectant during treatment.Conclusions: Most of the general dental practitioners took measures to establish and maintain aseptics during endodontic treatment, which infers an awareness of the importance of endodontic infection prevention and control. But the results were self-reported and there may be a gap between claimed and actual behaviour. Further studies using observation methodologies are needed to assess how infection control routines are performed in everyday clinical practice.
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| 3. |
- Björkner, Annika E, et al.
(författare)
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Det endodontiska arbetsfältet : aseptik och antiseptik i den kliniska vardagen
- 2017
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Ingår i: Aktuel Nordisk Odontologi. - : Universitetsförlaget. - 1902-3545. ; 42:01, s. 156-167
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Tidskriftsartikel (refereegranskat)abstract
- Inom tandvården sker behandlingar i en miljö som är mycket rik på mikroorganismer och med risk för smittspridning. För att minska denna risk behöver vi aktivt verka för att reducera smittkällor och bryta transportvägarna för mikroorganismer. Att arbeta aseptiskt och därmed «bevara det rena rent» är en grundläggande princip vid endodonti. Ett aseptiskt arbetsfält skapas med hjälp av kofferdam och antiseptika, och detta aseptiska arbetsfält måste upprätthållas under hela behandlingen. 90 % av svenska och 30 % av de danska tandläkarna använder kofferdam regelbundet. Trots införandet av flera nya tekniker för både instrumentering och rotfyllning under senare år ses inte någon minskad mängd apikal parodontit i befolkningen. Det är troligt att andra faktorer, som till exempel det aseptiska och antiseptiska tillvägagångssättet, kan spela en större roll för utfallet av den endodontiska behandlingen än vad som tidigare antagits.
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| 4. |
- Hallén, Ulrika, et al.
(författare)
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Binding of the periodontitis associated bacterium Porphyromonas gingivalis to glycoproteins from human epithelial cells
- 2008
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Ingår i: Oral Microbiology and Immunology. - 0902-0055. ; 23:5, s. 367-371
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: In the present study we examined the ability of the periodontal pathogen Porphyromonas gingivalis to adhere to glycoconjugates on intact cells and to protein preparations of epithelial cells (KB cells). Methods: The KB cell protein preparation was separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes by Western blotting. The membranes were used in overlay assays with labeled P. gingivalis. Flow cytometry was used to analyze attachment of bacteria to intact KB cells. Results: Glycoconjugate expression on the KB cells and in the protein preparation was confirmed. Binding was detected to several bands on the Western blots. Flow cytometry showed a distinct increase in fluorescence for strain FDC 381. Preincubation of the bacteria with mannose, fucose, N-acetylglucosamine and N-acetylgalactosamine inhibited the binding to KB cells by approximately 30% whereas preincubation with N-acetylneuraminic acid reduced the binding by 60%. Conclusion: These results indicate that carbohydrate structures are involved in the binding process of P. gingivalis to oral epithelial cells and that neuraminic acid plays a significant role in the adhesion process.
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| 5. |
- Hallén, Ulrika, et al.
(författare)
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Glycolipid binding epitopes involved in adherence of the periodontitis-associated bacterium Porphyromonas gingivalis.
- 2008
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Ingår i: Glycoconjugate journal. - : Springer Science and Business Media LLC. - 0282-0080 .- 1573-4986. ; 25:6, s. 561-572
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Tidskriftsartikel (refereegranskat)abstract
- The ability of the periodontal pathogen Porphyromonas gingivalis to use different glycolipid structures as receptors has previously been demonstrated. The bacterium adhered to acid and nonacid glycolipids originating from human organs and to nonacid glycolipids of porcine origin. The aim of the present study was to analyze these binding epitopes by structural characterization. Glycolipid fractions with positive bacterial binding from e.g. human and porcine origin, were purified by the high performance liquid chromatography technique and thereafter used in bacterial overlay assays with (35)S-labeled P. gingivalis. Purified fractions with positive binding were structurally characterized by proton nuclear magnetic resonance spectroscopy. Complementing thin-layer chromatograms and bacterial overlay assays with pure reference glycolipid fractions and competition experiments with lactose were performed to define potential receptors. The P. gingivalis binding epitopes, including cerebrosides with nonhydroxy fatty acids, lactosylceramide with hydroxy fatty acids, sulfatides, lacto-, neolacto- and gangliotetraosylceramides, are in several instances similar to those found for other bacteria, e.g. H. pylori, H. influenzae and N. meningitidis. In addition P. gingivalis also bound to the Galalpha4Gal epitope of the globo series of glycolipids. In the future these results may be valuable for development of new treatment strategies, such as anti-adhesion therapies and vaccines specifically directed against P. gingivalis infection.
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| 6. |
- Malmberg, Leona, 1973-, et al.
(författare)
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Endodontic operative field asepsis: a comparison between general dentists and specialists
- 2023
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Ingår i: Acta Odontologica Scandinavica. - : Taylor & Francis Group. - 0001-6357 .- 1502-3850. ; 81:8, s. 603-608
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Tidskriftsartikel (refereegranskat)abstract
- ObjectiveThe aim was to evaluate the establishment of an aseptic endodontic operative field in general dentistry by assessing general dentists' ability to reduce the amount of contamination to a non-cultivable level, and to compare the operative field asepsis at a general dentistry clinic with that at an endodontic specialist clinic.Materials and MethodsA total of 353 teeth were included in the study (153 in general dentistry, 200 at the specialist clinic). After isolation, control samples were taken, the operative fields disinfected with 30% hydrogen peroxide (1 min) followed by 5% iodine tincture or .5% chlorhexidine solution. Samples were collected from the access cavity area and buccal area, placed in a fluid thioglycolate medium, incubated (37 & DEG;, 7 d), evaluated for growth/non-growth.ResultsSignificantly more contamination was observed at the general dentistry clinic (31.6%, 95/301), than at the endodontic specialist clinic (7.0%, 27/386) (p <.001). In general dentistry, significantly more positive samples were collected in the buccal area than in the occlusal area. Significantly more positive samples were collected when the chlorhexidine protocol had been used, both in general dentistry (p <.001) and at the specialist clinic (p =.028).ConclusionsThe result from this study shows insufficient endodontic aseptic control in general dentistry. At the specialist clinic, both disinfection protocols were able to reduce the amount of microorganisms to a non-cultivable level. The observed difference between the protocols may not reflect a true difference in the effectiveness of the antimicrobial solutions, as confounding factors may have contributed to the result.
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| 7. |
- Malmberg, Leona, et al.
(författare)
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Establishment and maintenance of asepsis in endodontics – a review of the literature
- 2016
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Ingår i: Acta Odontologica Scandinavica. - : Informa UK Limited. - 0001-6357 .- 1502-3850. ; 74:6, s. 431-435
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Tidskriftsartikel (refereegranskat)abstract
- Abstract: Objectives: Successful endodontic treatment depends on effective measures to eliminate and prevent infection of root canals. Initially treatment should start with isolation and disinfection of the operating field. This review makes an inventory of the available knowledge regarding its establishment and maintenance. Materials and methods: A literature search was conducted in the PubMed database in order to identify clinical trials examining disinfection or unintentional contamination of the endodontic operative field. A list of 115 articles was obtained and screened. Five relevant articles were identified. These articles were read in full text. The reference lists from these articles were checked manually for additional studies and three studies were obtained. A total of eight articles met the inclusion criteria. Results: There was a great variety in terms of aim, method, and material of the included studies. None could prove a totally reliable aseptic operative field and not one chemical, or combination of chemicals, were found in more than one study. Conclusions: No study documented complete asepsis following initial disinfection, and no study could document predictable maintenance of an established bacteria-free surface. Critical appraisal and standardization of the disinfection and aseptic procedures in endodontics are needed. © 2016 Acta Odontologica Scandinavica Society.
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