SwePub - sökning: WFRF:(Brostedt E)

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1.	Lindblad, A, et al. (författare) Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum : partial characterization of a mutant unaffected in nitrogen fixation 1996 Ingår i: Molecular Microbiology. - : Wiley. - 0950-382X .- 1365-2958. ; 20:3, s. 559-568 Tidskriftsartikel (refereegranskat)abstract A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.

Lindblad, A, et al. (författare)
Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum : partial characterization of a mutant unaffected in nitrogen fixation
1996
Ingår i: Molecular Microbiology. - : Wiley. - 0950-382X .- 1365-2958. ; 20:3, s. 559-568
Tidskriftsartikel (refereegranskat)abstract
- A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.

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