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Sökning: WFRF:(DeBolt Seth)

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1.
  • Corbin, Kendall R., et al. (författare)
  • Grape marc as a source of carbohydrates for bioethanol : Chemical composition, pre-treatment and saccharification.
  • 2015
  • Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 193, s. 76-83
  • Tidskriftsartikel (refereegranskat)abstract
    • Global grape production could generate up to 13 Mt/yr of wasted biomass. The compositions of Cabernet Sauvignon (red marc) and Sauvignon Blanc (white marc) were analyzed with a view to using marc as raw material for biofuel production. On a dry weight basis, 31-54% w/w of the grape marc consisted of carbohydrate, of which 47-80% was soluble in aqueous media. Ethanol insoluble residues consisted mainly of polyphenols, pectic polysaccharides, heteroxylans and cellulose. Acid and thermal pre-treatments were investigated for their effects on subsequent cellulose saccharification. A 0.5M sulfuric acid pre-treatment yielded a 10% increase in the amount of liberated glucose after enzymatic saccharification. The theoretical amount of bioethanol that could be produced by fermentation of grape marc was up to 400 L/t. However, bioethanol from only soluble carbohydrates could yield 270 L/t, leaving a polyphenol enriched fraction that may be used in animal feed or as fertilizer.
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3.
  • Schrick, Kathrin, et al. (författare)
  • Deciphering the molecular functions of sterols in cellulose biosynthesis
  • 2012
  • Ingår i: Frontiers in Plant Science. - : Frontiers Media SA. - 1664-462X. ; 3, s. 84-
  • Tidskriftsartikel (refereegranskat)abstract
    • Sterols play vital roles in plant growth and development, as components of membranes and as precursors to steroid hormones. Analysis of Arabidopsis mutants indicates that sterol composition is crucial for cellulose biosynthesis. Sterols are widespread in the plasma membrane (PM), suggesting a possible link between sterols and the multimeric cellulose synthase complex. In one possible scenario, molecular interactions in sterol-rich PM microdomains or another form of sterol-dependent membrane scaffolding may be critical for maintaining the correct subcellular localization, structural integrity and/or activity of the cellulose synthase machinery. Another possible link may be through steryl glucosides, which could act as primers for the attachment of glucose monomers during the synthesis of beta - (1 -> 4) glucan chains that form the cellulose microfibrils. This mini-review examines genetic and biochemical data supporting the link between sterols and cellulose biosynthesis in cell wall formation and explores potential approaches to elucidate the mechanism of this association.
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