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Sökning: WFRF:(Del Barga I.)

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1.
  • Del Barga, I., et al. (författare)
  • Effects of algal extracts (Polysiphonia fucoides) on rainbow trout (Oncorhynchus mykiss): A biomarker approach
  • 2006
  • Ingår i: Marine Environmental Research. - : Elsevier BV. - 0141-1136. ; 62:Supplement 1
  • Tidskriftsartikel (refereegranskat)abstract
    • The genotoxicity of algal extracts (Polysiphonia fucoides) was investigated in erythrocytes of rainbow trout (Oncorhynchus mykiss). Trout were exposed to 0.5% of the algal extract for 7 days. Comet assay (alkaline and neutral versions) and Micronucleus test were used to assess DNA damage, and Diffusion Assay to detect apoptotic cells. EROD activities and oxidative stress parameters in rainbow trout liver were also measured. A significant induction of DNA single strand breaks comparable to the ones induced by the in vivo exposure to 20 mg/kg B[a]P was observed at the end of the treatment, while increases of double strand breaks and apoptotic cells were not observed. The absence of activation of antioxidant responses seems to underline a mechanism of action of the genotoxic algal extract which does not involve oxidative stress.
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2.
  • Frenzilli, G., et al. (författare)
  • DNA damage in eelpout (Zoarces viviparus) from Göteborg harbour
  • 2004
  • Ingår i: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. - : Elsevier BV. - 0027-5107. ; 552:1-2, s. 187-195
  • Tidskriftsartikel (refereegranskat)abstract
    • The relationship between DNA damage and the exposure of marine organisms to environmental contaminants was examined in the Göteborg harbour area. This research is part of a wider ecotoxicological study planned to evaluate the biological impact of chemical contamination in the River Göta estuary, following a bunker oil (10–100 tonnes) spill occurred in June 2003. Here we present data on the DNA strand breaks derived using the comet assay and the presence of apoptotic cells using the diffusion assay in nucleated erythrocytes of the eelpout (Zoarces viviparus) from the study area and at a clean reference site. Polycyclic aromatic hydrocarbon metabolites were also analyzed in the bile of exposed fish. The results showed a high level of damaged DNA, paralleled by a peak in bile PAH metabolites, in fish from the most impacted site, 3 weeks after the oil spill. A significant recovery was observed in specimens from the spill site, 5 months later, but not in fish caught in the middle part of Göteborg harbour, which is chronically subjected to heavy chemical pollution. The levels of apoptic cells did not show any marked variations, but a significant recovery was observed in fish from the oil impacted site 5 months after the spill
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  • Resultat 1-2 av 2
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tidskriftsartikel (2)
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refereegranskat (2)
Författare/redaktör
Förlin, Lars, 1950 (2)
Sturve, Joachim, 196 ... (2)
Frenzilli, G. (2)
Del Barga, I. (2)
Nigro, M. (2)
Scarcelli, V. (1)
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Asplund, L (1)
Scarelli, V (1)
Malmvarn, A (1)
Bolognesi, C. (1)
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Göteborgs universitet (2)
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Engelska (2)
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