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- Bengtsson, Bengt Å., et al.
(författare)
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Fjärilar
- 2010. - 1
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Ingår i: Rödlistade arter i Sverige 2010. - Uppsala : Artdatabanken i samarbete med Naturvårdsverket. - 9789188506351 ; , s. 361-392
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Bergstrand, Jan, et al.
(författare)
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Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Protein contents in platelets are frequently changed upon tumor development and metastasis. However, how cancer cells can influence protein-selective redistribution and release within platelets, thereby promoting tumor development, remains largely elusive. With fluorescence-based super-resolution stimulated emission depletion (STED) imaging we reveal how specific proteins, implicated in tumor progression and metastasis, re-distribute within platelets, when subject to soluble activators (thrombin, adenosine-diphosphate and thromboxaneA2), and when incubated with cancer (MCF-7, MDA-MB-231, EFO21) or non-cancer cells (184A1, MCF10A). Upon cancer cell incubation, the cell-adhesion protein P-selectin was found to re-distribute into circular nano-structures, consistent with accumulation into the membrane of protein-storing alpha-granules within the platelets. These changes were to a significantly lesser extent, if at all, found in platelets incubated with normal cells, or in platelets subject to soluble platelet activators. From these patterns, we developed a classification procedure, whereby platelets exposed to cancer cells, to non-cancer cells, soluble activators as well as non-activated platelets all could be identified in an automatic, objective manner. We demonstrate that STED imaging, in contrast to electron and confocal microscopy, has the necessary spatial resolution and labelling efficiency to identify protein distribution patterns in platelets and can resolve how they specifically change upon different activations. Combined with image analyses of specific protein distribution patterns within the platelets, STED imaging can thus have a role in future platelet-based cancer diagnostics and therapeutic monitoring. The presented approach can also bring further clarity into fundamental mechanisms for cancer cell-platelet interactions, and into non-contact cell-to-cell interactions in general.
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| 5. |
- Bergstrand, Jan, et al.
(författare)
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Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells
- 2019
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Ingår i: Nanoscale. - : ROYAL SOC CHEMISTRY. - 2040-3364 .- 2040-3372. ; 11:20, s. 10023-10033
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Tidskriftsartikel (refereegranskat)abstract
- Protein contents in platelets are frequently changed upon tumor development and metastasis. However, how cancer cells can influence protein-selective redistribution and release within platelets, thereby promoting tumor development, remains largely elusive. With fluorescence-based super-resolution stimulated emission depletion (STED) imaging we reveal how specific proteins, implicated in tumor progression and metastasis, re-distribute within platelets, when subject to soluble activators (thrombin, adenosine diphosphate and thromboxane A2), and when incubated with cancer (MCF-7, MDA-MB-231, EFO21) or non-cancer cells (184A1, MCF10A). Upon cancer cell incubation, the cell-adhesion protein P-selectin was found to re-distribute into circular nano-structures, consistent with accumulation into the membrane of protein-storing alpha-granules within the platelets. These changes were to a significantly lesser extent, if at all, found in platelets incubated with normal cells, or in platelets subject to soluble platelet activators. From these patterns, we developed a classification procedure, whereby platelets exposed to cancer cells, to non-cancer cells, soluble activators, as well as non-activated platelets all could be identified in an automatic, objective manner. We demonstrate that STED imaging, in contrast to electron and confocal microscopy, has the necessary spatial resolution and labelling efficiency to identify protein distribution patterns in platelets and can resolve how they specifically change upon different activations. Combined with image analyses of specific protein distribution patterns within the platelets, STED imaging can thus have a role in future platelet-based cancer diagnostics and therapeutic monitoring. The presented approach can also bring further clarity into fundamental mechanisms for cancer cell-platelet interactions, and into non-contact cell-to-cell interactions in general.
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| 6. |
- Bostner, Josefine, et al.
(författare)
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S6 kinase signaling: tamoxifen response and prognostic indication in two breast cancer cohorts
- 2015
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Ingår i: Endocrine-Related Cancer. - : BioScientifica. - 1351-0088 .- 1479-6821. ; 22:3, s. 331-343
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Tidskriftsartikel (refereegranskat)abstract
- Detection of signals in the mammalian target of rapamycin (mTOR) and the estrogen receptor (ER) pathways may be a future clinical tool for the prediction of adjuvant treatment response in primary breast cancer. Using immunohistological staining, we investigated the value of the mTOR targets p70-S6 kinase (S6K) 1 and 2 as biomarkers for tamoxifen benefit in two independent clinical trials comparing adjuvant tamoxifen with no tamoxifen or 5 years versus 2 years of tamoxifen treatment. In addition, the prognostic value of the S6Ks was evaluated. We found that S6K1 correlated with proliferation, HER2 status, and cytoplasmic AKT activity, whereas high protein expression levels of S6K2 and phosphorylated (p) S6K were more common in ER-positive, and low-proliferative tumors with pAKT-s473 localized to the nucelus. Nuclear accumulation of S6K1 was indicative of a reduced tamoxifen effect (hazard ratio (HR): 1.07, 95% CI: 0.53-2.81, P=0.84), compared with a significant benefit from tamoxifen treatment in patients without tumor S6K1 nuclear accumulation (HR: 0.42, 95% CI: 0.29-0.62, Pless than0.00001). Also S6K1 and S6K2 activation, indicated by pS6K-t389 expression, was associated with low benefit from tamoxifen (HR: 0.97, 95% CI: 0.50-1.87, P=0.92). In addition, high protein expression of S6K1, independent of localization, predicted worse prognosis in a multivariate analysis, P=0.00041 (cytoplasm), P=0.016 (nucleus). In conclusion, the mTOR-activated kinases S6K1 and S6K2 interfere with proliferation and response to tamoxifen. Monitoring their activity and intracellular localization may provide biomarkers for breast cancer treatment, allowing the identification of a group of patients less likely to benefit from tamoxifen and thus in need of an alternative or additional targeted treatment.
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| 7. |
- Carlén, Lina M, et al.
(författare)
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Proteome analysis of skin distinguishes acute guttate from chronic plaque psoriasis.
- 2005
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Ingår i: Journal of Investigative Dermatology. - 0022-202X .- 1523-1747. ; 124:1
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Tidskriftsartikel (refereegranskat)abstract
- Psoriasis is a disease with considerable heterogeneity in clinical presentation. This is the first study using two-dimensional gel electrophoresis to compare global protein expression patterns in lesional and non-lesional skin from subjects with acute guttate psoriasis associated with streptococcal throat infection and chronic plaque psoriasis. Samples from experimentally induced contact eczema and normal skin from healthy controls were also included. Proteins with statistically significant differences in expression were used in hierarchical cluster analyses resulting in separation of the different samples into groups. Chronic plaque and guttate psoriasis samples were distinctly separated, indicating that they represent discrete phenotypes at the protein expression level. Interestingly, there was a trend in which guttate psoriasis lesions clustered closer to eczema than to chronic plaque psoriasis lesions, indicating that the duration of the inflammatory reaction may affect clustering. Several of the differentially expressed proteins were identified by mass spectrometry.
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| 9. |
- Edvinsson, Rodney, 1971-, et al.
(författare)
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Sveriges tidiga formella penningväsende
- 2015
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Ingår i: Historisk Tidskrift. - 0345-469X .- 2002-4827. ; 135:3, s. 377-400
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Tidskriftsartikel (refereegranskat)abstract
- Föreliggande uppsats analyserar framväxten av ett formellt penningväsende i Sverige 995–1525. Ett av de tidigaste tecknen på monetarisering inträffade under andra halvan av 1200-talet och innebar att betalningar i bevarade källor huvudsakligen anges i mynt som räknats i stället för att vägas. Skillnaden mellan ett mynts nominella värde och dess värde som silver utgjordes av slagskatt. Myntförsämringen under 1300- och 1400-talen motverkades av att silvrets köpkraft ökade kraftigt.
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| 10. |
- Edvinsson, Rodney, 1971-, et al.
(författare)
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Swedish Payment Systems 995–1534
- 2010
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Ingår i: Exchange Rates, Prices, and Wages, 1277-2008. - Stockholm : Ekerlids förlag. - 9789170921247 - 9789189612440 ; , s. 67-132
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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