| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Chen, Yangyang, et al.
(författare)
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Wood-derived scaffolds decorating with nickel cobalt phosphate nanosheets and carbon nanotubes used as monolithic electrodes for assembling high-performance asymmetric supercapacitor
- 2023
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Ingår i: Chemical Engineering Journal. - : Elsevier BV. - 1385-8947 .- 1873-3212. ; 454
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Tidskriftsartikel (refereegranskat)abstract
- Lightweight carbonized wood (CW) loaded with pseudocapacitive materials has demonstrated excellent energy density. However, the direct loading of active materials usually results in poor rate performance and cycling stability. Herein, we fabricated a CW electrode with high loading of active materials and conductivity through chemical vapor deposition (CVD) and electrodeposition to sequentially incorporate carbon nanotubes (CNTs) and nickel-cobalt phosphate (NiCo-P) nanosheets. This integrated NiCo-P/CNT/CW electrode exhibited a promising areal capacitance of 11.2F cm-2 at a current density of 10 mA cm-2, and a notable capacitance retention rate of 86.6 % at 60 mA cm-2. The asymmetric supercapacitor (ASC) device assembled with the prepared electrode as anode and the self-activated carbonized wood (SCW) electrode as cathode delivers outstanding energy density of 5.74 mWh cm-3 (12.1 Wh kg -1) at power density of 18.75 mW cm-3 (39.5 W kg -1) while maintaining a high capacitance retention of 92.4 % after 10,000 charge-discharge cycles. This work provides an advanced approach for constructing supercapacitors with remarkable energy density and rate performance from the natural wood derived electrodes.
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| 3. |
- Hou, Yuanyuan, et al.
(författare)
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Comparative study of pressure-induced polymerization of C60 nanorods and single crystals
- 2007
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Ingår i: Journal of Physics Condensed Matter. - Bristol : Institute of Physics. - 0953-8984 .- 1361-648X. ; 19:42, s. 425207-
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, we report a comparative study of pressure-induced polymerization in C60 nanorods and bulk single crystals, treated simultaneously under various pressures and temperatures in the same experiment. For both materials, orthorhombic, tetragonal and rhombohedral phases have been produced under high pressure and high temperature. The structures have been identified and compared between the two sample types by Raman and photoluminescence spectroscopy. There are differences between the Raman and photoluminescence spectra from the two types of materials for all polymeric phases, but especially for the tetragonal phase. From the comparison between nanorods and bulk samples, we tentatively assign photoluminescence peaks for various polymeric phases.
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| 4. |
- Hou, Yuanyuan, et al.
(författare)
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Photoluminescence properties of high-pressure-polymerized C60 nanorods in the orthorhombic and tetragonal phases.
- 2006
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Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 89:18, s. 181925-
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Tidskriftsartikel (refereegranskat)abstract
- C60nanorods in two polymeric phases have been synthesized under differenthigh pressure and high temperature conditions. Orthorhombic and tetragonal phaseshave been identified from Raman spectra. The rod shape canbe kept under quasihydrostatic pressure. The photoluminescence intensity of thepolymeric C60 nanorods has been greatly enhanced compared with thatof pristine C60 nanorods. The main fluorescence band shifted from730 nm in the unpolymeric phase to 748 nm and near infrared780 nm in the orthorhombic and tetragonal phases, respectively. The enhancedphotoluminescence with tunable frequency for different polymeric C60 nanorods suggestspotential applications in luminescent nanomaterials.
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| 5. |
- Hou, Yuanyuan, et al.
(författare)
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Pressure-induced polymerization of nano- and submicrometer C60 rods into a rhombohedral phase
- 2006
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Ingår i: Chemical Physics Letters. - : Elsevier BV. - 0009-2614. ; 423:1-3, s. 215-219
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Tidskriftsartikel (refereegranskat)abstract
- C-60 rods with nano- and submicrometer dimensions were polymerized at 4.5 GPa and 973 K. Raman spectroscopy showed that the material transformed into a rhombohedral polymeric structure under high pressure and high temperature (HPHT) conditions. The HPHT polymerized samples keep the original rod shape. The position dependence of photoluminescence (PL) spectra have been studied on the rhombohedral polymer C60 rods at room temperature. The intensity of the 1.74 eV peak from the rhombohedral phase varies systematically between the edge of and the middle of the rod. Our results indicate that the 1.74 eV peak originates from surface defects.
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| 6. |
- Hu, Xiangzhao, et al.
(författare)
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Boosting Industrial-Level CO2 Electroreduction of N-Doped Carbon Nanofibers with Confined Tin-Nitrogen Active Sites via Accelerating Proton Transport Kinetics
- 2023
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Ingår i: Advanced Functional Materials. - : John Wiley and Sons Inc. - 1616-301X .- 1616-3028. ; 33:4
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Tidskriftsartikel (refereegranskat)abstract
- The development of highly efficient robust electrocatalysts with low overpotential and industrial-level current density is of great significance for CO2 electroreduction (CO2ER), however the low proton transport rate during the CO2ER remains a challenge. Herein, a porous N-doped carbon nanofiber confined with tin-nitrogen sites (Sn/NCNFs) catalyst is developed, which is prepared through an integrated electrospinning and pyrolysis strategy. The optimized Sn/NCNFs catalyst exhibits an outstanding CO2ER activity with the maximum CO FE of 96.5%, low onset potential of −0.3 V, and small Tafel slope of 68.8 mV dec−1. In a flow cell, an industrial-level CO partial current density of 100.6 mA cm−2 is achieved. In situ spectroscopic analysis unveil the isolated Sn-N site acted as active center for accelerating water dissociation and subsequent proton transport process, thus promoting the formation of intermediate *COOH in the rate-determining step for CO2ER. Theoretical calculations validate pyrrolic N atom adjacent to the Sn-N active species assisted reducing the energy barrier for *COOH formation, thus boosting the CO2ER kinetics. A Zn-CO2 battery is designed with the cathode of Sn/NCNFs, which delivers a maximum power density of 1.38 mW cm−2 and long-term stability.
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| 7. |
- Lensink, Marc F., et al.
(författare)
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Impact of AlphaFold on structure prediction of protein complexes: The CASP15-CAPRI experiment
- 2023
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Ingår i: Proteins. - : WILEY. - 0887-3585 .- 1097-0134.
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Tidskriftsartikel (refereegranskat)abstract
- We present the results for CAPRI Round 54, the 5th joint CASP-CAPRI protein assembly prediction challenge. The Round offered 37 targets, including 14 homodimers, 3 homo-trimers, 13 heterodimers including 3 antibody-antigen complexes, and 7 large assemblies. On average similar to 70 CASP and CAPRI predictor groups, including more than 20 automatics servers, submitted models for each target. A total of 21 941 models submitted by these groups and by 15 CAPRI scorer groups were evaluated using the CAPRI model quality measures and the DockQ score consolidating these measures. The prediction performance was quantified by a weighted score based on the number of models of acceptable quality or higher submitted by each group among their five best models. Results show substantial progress achieved across a significant fraction of the 60+ participating groups. High-quality models were produced for about 40% of the targets compared to 8% two years earlier. This remarkable improvement is due to the wide use of the AlphaFold2 and AlphaFold2-Multimer software and the confidence metrics they provide. Notably, expanded sampling of candidate solutions by manipulating these deep learning inference engines, enriching multiple sequence alignments, or integration of advanced modeling tools, enabled top performing groups to exceed the performance of a standard AlphaFold2-Multimer version used as a yard stick. This notwithstanding, performance remained poor for complexes with antibodies and nanobodies, where evolutionary relationships between the binding partners are lacking, and for complexes featuring conformational flexibility, clearly indicating that the prediction of protein complexes remains a challenging problem.
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| 8. |
- Liu, Bingbing, et al.
(författare)
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High pressure and high temperature induced polymeric C60 nanocrystal
- 2008
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Ingår i: Diamond and related materials, vol. 17, issue 4-5. - Amsterdam : Elsevier B.V.. ; , s. 620-623
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Konferensbidrag (refereegranskat)abstract
- In this paper, C60 nanosheets with polymeric phases have been obtained under various high pressures and high temperatures, including orthorhombic and tetragonal polymeric phases. The structures have been identified and compared with those of nanorods by photoluminescence and Raman spectroscopies. The main fluorescence band shifted from 1.70 eV in the monomeric phase to near infrared in the polymeric phase when pressure and temperature were increased. The difference of photoluminescence and Raman spectra between nanosheets and nanorods samples treated under the same conditions is probably caused by different polymerization degree in these samples because of different shapes.
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| 9. |
- Liu, Bingbing, et al.
(författare)
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High pressure and high temperature induced polymeric C60 nanorods and their photoluminiscence properties.
- 2007
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Ingår i: Materials Research at High Pressure. - Warrendale : Maerials Research Society. - 9781558999442 ; , s. 99-103
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Konferensbidrag (refereegranskat)abstract
- We report that C60 nanorods of three polymeric phases have been synthesized under high pressure and high temperature and that the photoluminescence properties of these polymeric nanorods also have been studied. C60 nanorods were fabricated by a facile solution evaporation method. Raman spectra indicate that this material transformed into orthorhombic, tetragonal and rhombohedral polymeric phases under various high pressure and high temperature conditions. SEM measurements show that the shape of the nanorods can be kept under quasi-hydrostatic pressure condition. The photoluminescence intensity of the polymeric C60 nanorods has been enhanced compared with that of the pristine C60 nanorods. The main fluorescence band shifted from 1.70 eV in the monomeric phase to near infrared in the polymeric phase when pressure and temperature were increased. The enhanced fluorescence with adjustable frequency for different polymeric C60 nanorods makes possible potential application in luminescent nanomaterials.
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| 10. |
- Liu, Bingbing, et al.
(författare)
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High-pressure induced polymerization of C60 nanorods
- 2005
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Ingår i: Proceedings of the Joint 20th AIRAPT and 43rd EHPRG Conf. on High Pressure Science and Technology, Karlsruhe 2005. - 3923704496 ; , s. Nanomat-O133
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- C60 nanorods, about 200 nm in diameter and several micrometers in length and with a rectangular cross section, have been synthesized by a simple solution-growth method. Raman spectroscopy and TEM indicate that the C60 nanorods have a fcc crystalline structure similar to that of pristine C60. Nanorods were polymerized at high temperature and high pressure below 2 GPa. Two different polymeric structures, orthorhombic and tetragonal as determined by Raman spectroscopy, have been obtained under different high temperature and high pressure conditions. The high-pressure induced polymeric samples keep the original nanometer scale rod shape.
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