| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Zhang, Lin, et al.
(författare)
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Outage Probability Analysis and Optimization in Downlink NOMA Systems with Cooperative Full-duplex Relaying
- 2017
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Ingår i: 2017 IEEE 86th Vehicular Technology Conference (VTC-FALL). - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 1-5
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Konferensbidrag (refereegranskat)abstract
- We study a downlink non-orthogonal multiple access (NOMA) system with cooperative full-duplex relaying, where the near user in terms of the base station (BS) is enabled to act as a full-duplex relay for the far user. In particular, we first derive the outage probability with closed-form expressions when the power allocations at the BS and relay (or the near user) are fixed. Then, we analytically obtain the optimal power allocations with closed-form expressions at the BS and relay to minimize the outage probability. Numerical results validate the correctness of the theoretical analysis and demonstrate the advantages of the proposed algorithms over the state of arts.
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| 3. |
- Zhang, Lin, et al.
(författare)
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Performance Analysis and Optimization in Downlink NOMA Systems With Cooperative Full-Duplex Relaying
- 2017
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Ingår i: IEEE Journal on Selected Areas in Communications. - : IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC. - 0733-8716 .- 1558-0008. ; 35:10, s. 2398-2412
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Tidskriftsartikel (refereegranskat)abstract
- We study a downlink non-orthogonal multiple access system with cooperative full-duplex relaying, where the near user in terms of the base station (BS) is enabled to act as a full-duplex relay for the far user. In particular, we first derive the outage probability and ergodic sum rate with closed-form expressions when the power allocations at the BS and relay (or the near user) are fixed. Then, we analytically obtain the optimal power allocations with closed-form expressions at the BS and relay to minimize the outage probability. Furthermore, by taking the fairness between the near user and far user into account, we characterize the optimal power allocations with closed-form expressions at the BS and relay to maximize the minimum achievable rate of users. Simulation results validate the correctness of the theoretical analysis and demonstrate the advantages of the proposed algorithms over the state of the art.
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