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- Benrick, Anna, 1979, et al.
(författare)
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Electroacupuncture mimics exercise-induced changes in skeletal muscle gene expression in polycystic ovary syndrome women.
- 2020
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Ingår i: The Journal of clinical endocrinology and metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 105:6, s. 2027-2041
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Tidskriftsartikel (refereegranskat)abstract
- Autonomic nervous system activation mediates the increase in whole-body glucose uptake in response to electroacupuncture but the mechanisms are largely unknown.To identify the molecular mechanisms underlying electroacupuncture-induced glucose uptake in skeletal muscle in insulin-resistant overweight/obese women with and without polycystic ovary syndrome (PCOS).Participants: In a case-control study, skeletal muscle biopsies were collected from 15 women with PCOS and 14 controls before and after electroacupuncture. Gene expression and methylation was analyzed using Illumina BeadChips arrays.A single bout of electroacupuncture restores metabolic and transcriptional alterations and induces epigenetic changes in skeletal muscle. Transcriptomic analysis revealed 180 unique genes (q<0.05) whose expression was changed by electroacupuncture, with 95% of the changes towards a healthier phenotype. We identified DNA methylation changes at 304 unique sites (q<0.20), and these changes correlated with altered expression of 101 genes (p<0.05). Among the 50 most upregulated genes in response to electroacupuncture, 38% were also upregulated in response to exercise. We identified a subset of genes that were selectively altered by electroacupuncture in women with PCOS. For example, MSX1 and SRNX1 were decreased in muscle tissue of women with PCOS and were increased by electroacupuncture and exercise. siRNA-mediated silencing of these two genes in cultured myotubes decreased glycogen synthesis, supporting a role for these genes in glucose homeostasis.Our findings provide evidence that electroacupuncture normalizes gene expression in skeletal muscle in a manner similar to acute exercise. Electroacupuncture might therefore be a useful way of assisting those who have difficulties performing exercise.
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| 2. |
- Benrick, Anna, 1979-, et al.
(författare)
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Electroacupuncture mimics exercise-induced changes in skeletal muscle gene expression in women with polycystic ovary syndrome
- 2020
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - : Oxford University Press. - 0021-972X .- 1945-7197. ; 105:6, s. 2027-2041
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Tidskriftsartikel (refereegranskat)abstract
- ContextAutonomic nervous system activation mediates the increase in whole-body glucose uptake in response to electroacupuncture but the mechanisms are largely unknown.ObjectiveTo identify the molecular mechanisms underlying electroacupuncture-induced glucose uptake in skeletal muscle in insulin-resistant overweight/obese women with and without polycystic ovary syndrome (PCOS).Design/ParticipantsIn a case-control study, skeletal muscle biopsies were collected from 15 women with PCOS and 14 controls before and after electroacupuncture. Gene expression and methylation was analyzed using Illumina BeadChips arrays.ResultsA single bout of electroacupuncture restores metabolic and transcriptional alterations and induces epigenetic changes in skeletal muscle. Transcriptomic analysis revealed 180 unique genes (q < 0.05) whose expression was changed by electroacupuncture, with 95% of the changes towards a healthier phenotype. We identified DNA methylation changes at 304 unique sites (q < 0.20), and these changes correlated with altered expression of 101 genes (P < 0.05). Among the 50 most upregulated genes in response to electroacupuncture, 38% were also upregulated in response to exercise. We identified a subset of genes that were selectively altered by electroacupuncture in women with PCOS. For example, MSX1 and SRNX1 were decreased in muscle tissue of women with PCOS and were increased by electroacupuncture and exercise. siRNA-mediated silencing of these 2 genes in cultured myotubes decreased glycogen synthesis, supporting a role for these genes in glucose homeostasis.ConclusionOur findings provide evidence that electroacupuncture normalizes gene expression in skeletal muscle in a manner similar to acute exercise. Electroacupuncture might therefore be a useful way of assisting those who have difficulties performing exercise.
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| 3. |
- Bjerg, Anders, et al.
(författare)
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Shorter time to clinical decision in work-related asthma using a digital tool
- 2020
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Ingår i: ERJ open research. - Lausanne, Switzerland : European Respiratory Society (ERS). - 2312-0541. ; 6:3
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- PEF curves are a useful but cumbersome tool in diagnosing work-related asthma. Using a digital spirometer and smartphone app, time to clinical decision could be shortened by 6-7 weeks. Physician's time spent analysing PEF data is also shortened.
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| 8. |
- Forsberg, Jonas, 1982-
(författare)
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Energy transition in transportation : Applying TIMES-based energy system optimisation models to sub-national levels
- 2021
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Transportation is embedded in the fabric of society and a key enabler of socio-economic development, but it is also a major source of carbon dioxide (CO2) and local air pollution (AP). Cities collectively account for around three quarters of total energy-related CO2 emissions, and the negative health impacts from local APs are most felt in dense urban environments. Thus, transitioning away from current fossil fuel regime in urban transportation is necessary to address both global and local challenges. Mathematical models as energy system optimisation models (ESOMs) are commonly applied to explore contrasting energy futures and to provide insights on how the energy system (or specific sub-sectors) may evolve under different conditions. However, ‘typical’ national level models are not fully adapted to capture the characteristics of local (city) transportation, and previous city-level ESOM based analyses have focused on decarbonisation of local energy systems, thus omitting other local policy considerations as e.g. air quality, and several studies excluded transportation altogether. In this thesis, a generic city-level ESOM framework (TIMES-City) was further adapted and used to provide policy-relevant insights on the anticipated energy transition of the local transport sector. The underlying work rests on a systems analysis approach, building on careful consideration of the overall system performance and boundaries, understanding of specific system characteristics, and challenges and opportunities facing local ‘system managers’; this has implications for model representation and for quantitative and qualitative modelling assumptions. Further, availability and quality of local transport, energy and emission data needed to calibrate models poses significant challenges, and considerable effort was also put on producing projections for future transport demand (a key model input), using lessons and input data from traditional transport demand models. These considerations were addressed in Paper I. The model was then applied to two different cases (in Sweden) to explore potential conflicts and co-benefits between ambitious climate targets and deep cuts in APs (Paper II), and to assess the roles of local and regional governments in CO2 mitigation when also considering ambitious national-scale policies (Paper III). The results of Paper II indicate that substituting fossil fuels for biofuels in conventional vehicles is the least-cost decarbonisation pathway, however this produces only minor or even negative benefits to air quality. While, zero-emission vehicles cut all local tail-pipe emissions, but their total impact on climate change mitigation is determined by upstream impacts from the conversion and distribution of energy carriers. Thus, ensuring low levels of total CO2 and APs from transportation calls for re-coupling of the local and global responsibilities and motivations into comprehensive mitigation strategies. The results of Paper III indicate that current Swedish national mitigation measures will drive down CO2 emissions in transportation considerably, but biofuel availability and BEV (battery electric vehicles) costs are critical for the rate and extent of the transition, while locally and regionally determined measures to enable shifts (from car) to active travelling, public transportation and home-based work have a much more limited direct impact. Nonetheless, these measures, along with city investments in BEVs and charging infrastructure which pave the way also for residents and local businesses, can help to reduce overall energy intensity of the transport sector, thus slowing down growth in fuel demand and contribute to reaching ambitious climate targets with limited renewable resources (as e.g. biofuels). The two studies (Papers II and III) illustrate the usefulness of applying comprehensive ESOMs also at sub-national levels, providing insights on both global and local sustainability implications as well as deeper understanding of the roles of local and regional decision-makers in enabling and supporting low-carbon transitions in transportation.
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| 9. |
- Forsberg, Jonas, et al.
(författare)
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Exploring local CO2 mitigation strategies in transportation under ambitious national policies – A participatory energy system modelling approach
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Sweden has adopted ambitious climate targets which require deep cuts in CO2 emissions from transportation. To achieve this, it has introduced policies to incentivise the electrification of road vehicles and progressive blending-in of biofuels. Many local governments have committed to do their share, but the impact of local strategies remains unclear. Here, locally rooted mitigation strategies are explored by applying a novel approach to participatory energy-system modelling via multiple researcher–practitioner interactions stretching over two years. A comprehensive Energy System Optimisation Model (ESOM) was used to assess the impact of local mitigation strategies employed in 15 municipalities, under a set of existing ambitious national policies. The results show that, while local strategies can help reduce energy-related CO2 emissions, the direct impact on such emissions is reduced under ambitious national policies. Still, local strategies to increase public transport and active travelling, and facilitate remote working also have local benefits as improved air quality and reduced traffic noise. Local policymakers should put stronger emphasis on such benefits. Finally, the long-term participatory approach also helped to build practitioners’ trust in the modelling process and created an environment for mutual learning and deeper understanding of climate mitigation options. These benefits justify the resource-intensive process.
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