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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Li, Shu, et al.
(författare)
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Comparative efficacy and safety of urate-lowering therapy for the treatment of hyperuricemia : a systematic review and network meta-analysis
- 2016
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- The prevalence of hyperuricemia and gout has been increasing, but the comparative effectiveness and safety of different treatments remain uncertain. We aimed to compare the effectiveness and safety of different treatments for hyperuricemia using network meta-analysis methodology. We systematically reviewed fifteen randomized controlled trials (involving 7,246 patients through January 2016) that compared the effects of different urate-lowering drugs (allopurinol, benzbromarone, febuxostat, pegloticase and probenecid) on hyperuricemia. Drug efficacy and safety, as outcomes, were measured by whether the target level of serum urate acid was achieved and whether any adverse events occurred, respectively. We derived pooled effect sizes expressed as odds ratios (ORs) and 95% confidence intervals (CIs). The efficacy and safety of the drugs were ranked by cumulative ranking probabilities. Our findings show that febuxostat, benzbromarone, probenecid, pegloticase, and allopurinol were all highly effective at reducing the risk of hyperuricemia compared to placebo. Febuxostat had the best efficacy and safety compared to the other drugs. Furthermore, febuxostat 120 mg QD was more effective at achieving urate-lowering targets (OR: 0.17, 95% CI: 0.12-0.24) and safer (OR: 0.72, 95% CI: 0.56-0.91) than allopurinol.
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| 4. |
- Attané, Isabelle, et al.
(författare)
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Being a single man in rural China
- 2018
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Ingår i: Population and Societies. - 0184-7783. ; :557, s. 1-4
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Tidskriftsartikel (refereegranskat)abstract
- Those who are single in rural China are almost exclusively men. The presence of excess men in the population combines with internal migration and women's quest for upward social mobility through marriage to explain this phenomenon. The DefiChine survey sheds light on the situation of single men in three rural districts of Shaanxi. One of its findings is that, although the shortage of women does create competition between men looking for a wife, the social and economic dimensions of male singlehood cannot be ignored.
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| 5. |
- Brownstein, Catherine A., et al.
(författare)
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An international effort towards developing standards for best practices in analysis, interpretation and reporting of clinical genome sequencing results in the CLARITY Challenge
- 2014
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Ingår i: Genome Biology. - : Springer Science and Business Media LLC. - 1465-6906 .- 1474-760X. ; 15:3, s. R53-
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Tidskriftsartikel (refereegranskat)abstract
- Background: There is tremendous potential for genome sequencing to improve clinical diagnosis and care once it becomes routinely accessible, but this will require formalizing research methods into clinical best practices in the areas of sequence data generation, analysis, interpretation and reporting. The CLARITY Challenge was designed to spur convergence in methods for diagnosing genetic disease starting from clinical case history and genome sequencing data. DNA samples were obtained from three families with heritable genetic disorders and genomic sequence data were donated by sequencing platform vendors. The challenge was to analyze and interpret these data with the goals of identifying disease-causing variants and reporting the findings in a clinically useful format. Participating contestant groups were solicited broadly, and an independent panel of judges evaluated their performance. Results: A total of 30 international groups were engaged. The entries reveal a general convergence of practices on most elements of the analysis and interpretation process. However, even given this commonality of approach, only two groups identified the consensus candidate variants in all disease cases, demonstrating a need for consistent fine-tuning of the generally accepted methods. There was greater diversity of the final clinical report content and in the patient consenting process, demonstrating that these areas require additional exploration and standardization. Conclusions: The CLARITY Challenge provides a comprehensive assessment of current practices for using genome sequencing to diagnose and report genetic diseases. There is remarkable convergence in bioinformatic techniques, but medical interpretation and reporting are areas that require further development by many groups.
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| 6. |
- Li, Weidong, et al.
(författare)
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Intelligent Optimisation for Integrated Process Planning and Scheduling
- 2011
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Ingår i: Multi-objective Evolutionary Optimisation for Product Design and Manufacturing. - London : Springer London. - 9780857296177 - 9780857296528 ; , s. 305-324
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Bokkapitel (refereegranskat)abstract
- Traditionally, process planning and scheduling were performed sequentially, where scheduling was executed after process plans had been generated. Considering the fact that the two functions are usually complementary, it is necessary to integrate them more tightly so that the performance of a manufacturing system can be improved greatly. In this chapter, a multi-agent-based framework has been developed to facilitate the integration of the two functions. In the framework, the two functions are carried out simultaneously, and an optimization agent based on evolutionary algorithms is used to manage the interactions and communications between agents to enable proper decisions to be made. To verify the feasibility and performance of the proposed approach, experimental studies conducted to compare this approach and some previous works are presented. The experimental results show the proposed approach has achieved significant improvement.
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| 8. |
- Lu, Xin, et al.
(författare)
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A Generic Digital Twin Framework for Collaborative Supply Chain Development
- 2022
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Ingår i: 2022 5th International Conference on Computing and Big Data (ICCBD 2022). - : IEEE. - 9781665457163 - 9781665457156 - 9781665457170 ; , s. 177-181
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Konferensbidrag (refereegranskat)abstract
- Current Supply Chains (SCs) are complex and diverse along with fragile to SC disruptions. This leads urgently needs to develop an intelligent, transparent, collaborative and resilient SC system to cope with unexpected SC disruptions. Digital twin (DT) is one of the most promising solutions to develop smart SCs that has been extensively studied recent years. However, SCDT paradigm is still at an early stage. This paper presents a generic and modularized five layers DT framework to provide a flexible and collaborative solution, which can be compatible with different DT systems in various SCs. The feasibility of the proposed framework is validated through a practical implementation in a distributed eyewear industry.
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| 9. |
- Zhang, Liang, et al.
(författare)
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Deep Learning for Additive Screening in Perovskite Light-Emitting Diodes
- 2022
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Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 61:37
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Tidskriftsartikel (refereegranskat)abstract
- Additive engineering with organic molecules is of critical importance for achieving high-performance perovskite optoelectronic devices. However, experimentally finding suitable additives is costly and time consuming, while conventional machine learning (ML) is difficult to predict accurately due to the limited experimental data available in this relatively new field. Here, we demonstrate a deep learning method that can predict the effectiveness of additives in perovskite light-emitting diodes (PeLEDs) with a high accuracy up to 96 % by using a small dataset of 132 molecules. This model can maximize the information of the molecules and significantly mitigate the duplicated problem that usually happened with previous models in ML for molecular screening. Very high efficiency PeLEDs with a peak external quantum efficiency up to 22.7 % can be achieved by using the predicated additive. Our work opens a new avenue for further boosting the performance of perovskite optoelectronic devices.
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| 10. |
- Zhang, Wei, et al.
(författare)
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Thermal detection of surface plasmons on gold nanohole arrays
- 2012
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Ingår i: Chinese Science Bulletin. - : Springer Science and Business Media LLC. - 1001-6538 .- 1861-9541. ; 57:1, s. 68-71
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Tidskriftsartikel (refereegranskat)abstract
- We used a thin-film thermocouple to detect the thermal effect of surface plasmons excited in Au nanohole array structures. We found that the thermal electromotive force of Au film with periodic nanohole structures is three times greater than that of a bare Au film for 785-nm laser excitation at a given power. This effect is caused by the resonant excitation of localized surface plasmons in the nanoholes. In addition, we found that the thermal electromotive force (EMF) of the Au film with dumbbell-like nanohole arrays depends strongly on the incident polarization. The thermal EMF is the greatest when the excitation light is polarized perpendicular to the long axis of the dumbbell.
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