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- Frisk, Henrik, et al.
(författare)
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Acts of Creation : Introduction
- 2015
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Ingår i: Acts of Creation : Thoughts on artistic research supervision - Thoughts on artistic research supervision. - 9789187483165 ; , s. 7-18
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Betancourt, Lazaro Hiram, et al.
(författare)
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The human melanoma proteome atlas-Defining the molecular pathology
- 2021
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Ingår i: Clinical and Translational Medicine. - : Wiley. - 2001-1326. ; 11:7, s. 1-20
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Tidskriftsartikel (refereegranskat)abstract
- The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in-depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectrum of diverse, surgically isolated melanoma tumors originating from multiple body locations. More than 15,500 proteoforms were identified by mass spectrometry, from which chromosomal and subcellular localization was annotated within both primary and metastatic melanoma. The data generated by global proteomic experiments covered 72% of the proteins identified in the recently reported high stringency blueprint of the human proteome. This study contributes to the NIH Cancer Moonshot initiative combining detailed histopathological presentation with the molecular characterization for 505 melanoma tumor samples, localized in 26 organs from 232 patients.
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| 5. |
- Fehniger, Thomas E, et al.
(författare)
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Exploring the context of the lung proteome within the airway mucosa following allergen challenge.
- 2004
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 3:2, s. 307-320
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Forskningsöversikt (refereegranskat)abstract
- The lung proteome is a dynamic collection of specialized proteins related to pulmonary function. Many cells of different derivations, activation states, and levels of maturity contribute to the changing environment, which produces the lung proteome. Inflammatory cells reacting to environmental challenge, for example from allergens, produce and secrete proteins which have profound effects on both resident and nonresident cells located in airways, alveoli, and the vascular tree which provides blood cells to the parenchyma alveolar bed for gas exchange. In an experimental model of allergic airway inflammation, we have compared control and allergen challenged lung compartments to determine global protein expression patterns using 2D-gel electrophoresis and subsequent spot identification by MS/MS mass spectrometry. We have then specifically isolated the epithelial mucosal layer, which lines conducting airways, from control and allergen challenged lungs, using laser capture technology and performed proteome identification on these selected cell samples. A central component of our investigations has been to contextually relate the histological features of the dynamic pulmonary environment to the changes in protein expression observed following challenge. Our results provide new information of the complexity of the submucosa/epithelium interface and the mechanisms behind the transformation of airway epithelium from normal steady states to functionally activated states.
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| 6. |
- Lindberg, Claes, et al.
(författare)
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Liquid chromatography-tandem mass spectrometry approach for quantification of mucins from sputum using C-13,N-15-labeled peptides as internal standards
- 2013
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 1096-0309 .- 0003-2697. ; 434:1, s. 84-92
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Tidskriftsartikel (refereegranskat)abstract
- Mucins are of great interest owing their involvement in physiological and pathological processes in the airways. A method which allows accurate quantification of such proteins in sputum samples may be helpful for research in this field. A liquid chromatographic single reaction monitoring (SRM) method was developed for the quantification of two mucins, MUC5AC and MUC5B, in induced sputum samples. Sample preparation for the assay included solubilization, reduction and alkylation prior to tryptic digestion. Solid phase extraction using C(18) sorbent was used for sample clean up prior to the LC-MS/MS analysis. A cysteine containing peptide was selected for quantification of MUC5AC protein, whereas a non-cysteine peptide was used for the quantification of MUC5B protein. Stable isotope-labeled synthetic peptides were used as internal standards and linear calibration curves were constructed in the range of 0.3 - 40 pmol/L. Both mucins could be determined with a precision of 6-19% and an accuracy of 98-114%. The method is transferable to robotics and is suitable to be run in a 96-well format.
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| 7. |
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| 8. |
- Lindberg-Sand, Åsa, et al.
(författare)
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Contextual and conceptual ambiguities in supervision of artistic research : experiences from Konstnärliga forskarskolan
- 2015
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Ingår i: Acts of Creation : thoughts on artistic research supervision. - 9789187483165 ; , s. 189-220
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Bokkapitel (refereegranskat)abstract
- To accept the nomination to become the supervisor of a PhD candidate is not only to step into a specific, challenging and potentially rewarding relationship, but also to enter a new zone of uncertainty: what kind of contribution to this relationship is expected from me? In what ways is this new professional task framed and supported by the institutional context to which I belong? What do I gain from becoming a supervisor? In this chapter we draw together our experiences of spending many hundreds of hours listening to and discussing with supervisors of PhD candidates from a wide variety of research domains. We use our experiences from ten years of arranging pedagogical support for supervisors in the form of courses and seminars, and use what we have learned in these meeting places as material to describe how we perceive the cultural and contextual conditions for supervising PhD candidates both in general and in the emerging field of artistic research. The aim is therefore not to describe or evaluate our pedagogical efforts, but to use what we have encountered and reflected on in seminars with supervisors over the years. Another aim is to shed some light on the cultural and contextual conditions for supervising artistic research by comparing and contrasting the situation of the supervisors in this relatively new field with the situation in other fields of research. A more personal aim is to try to disentangle some of the persistent puzzlement that followed us through the years with Konstnärliga forskarskolan (KFS). Why was it that over and over again we were troubled by a feeling that the supervisors, compared to our expectations, were not regarded as being crucially important for the development of artistic research?In this chapter we will first discuss the importance of supervisors for doctoral education and for the regeneration and development of research, both in general and for new fields, with continuous references to artistic research, as it was mirrored to us through KFS. We will then continue along a road where we look at a number of contextual conditions (such as the learning trajectories of artists) and practiced concepts (such as interdisciplinarity and practice-based research), where we have experienced intriguing differences in artistic research when compared to other research environments. Finally, we offer two scenarios staging the institutional and cultural conditions for supervising artistic research in two quite different ways. They are constructed as ideal types and as a tool to disentangle some of the ambiguities supervisors have to face in the real settings where they meet their PhD candidates, and where everything is mixed up, as in life itself. Finally, we end our chapter by pointing out a ghost scenario, which we hope will disappear with the continued development of artistic research.
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| 10. |
- Malmström, Johan, et al.
(författare)
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Transforming growth factor-beta 1 specifically induce proteins involved in the myofibroblast contractile apparatus
- 2004
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Ingår i: Molecular & Cellular Proteomics. - 1535-9484. ; 3:5, s. 466-477
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Tidskriftsartikel (refereegranskat)abstract
- Transforming growth factor-beta(1) (TGF-beta(1)) induces alpha-smooth muscle actin (alpha-SMA) and collagen synthesis in fibroblast both in vivo and in vitro and plays a significant role in tissue repair and the development of fibrosis. During these processes the fibroblasts differentiate into activated fibroblasts (so called myofibroblasts), characterized by increased alpha-SMA expression. Because TGF-beta(1) is considered the main inducer of the myofibroblast phenotype and cytoskeletal changes accompany this differentiation, the main objective of this investigation was to study how TGF-beta(1) alters protein expression of cytoskeletal-associated proteins. Metabolic labeling of cell cultures by [(35)S]methionine, followed by protein separation on two-dimensional gel electrophoresis, displayed approximately 2500 proteins in the pI interval of 3-10. Treatment of TGF-beta(1) led to specific spot pattern changes that were identified by mass spectrometry and represent specific induction of several members of the contractile apparatus such as calgizzarin, cofilin, and profilin. These proteins have not previously been shown to be regulated by TGF-beta(1), and the functional role of these proteins is to participate in the depolymerization and stabilization of the microfilaments. These results show that TGF-beta(1) induces not only alpha-SMA but a whole set of actin-associated proteins that may contribute to the increased contractile properties of the myofibroblast. These proteins accompany the induced expression of alpha-SMA and may participate in the formation of stress fibers, cell contractility, and cell spreading characterizing the myofibroblasts phenotype.
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