| 1. |
- Lundstig, Annika, et al.
(författare)
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No detection of SV40 DNA in mesothelioma tissues from a high incidence area in Sweden.
- 2007
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Ingår i: Anticancer research. - 1791-7530 .- 0250-7005. ; 27:6B, s. 4159-4161
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Tidskriftsartikel (refereegranskat)abstract
- Simian virus 40 (SV40), a polyoma virus of the rhesus macaque was discovered in 1960 as a contaminant of human polio vaccines produced in monkey cells. A number of studies have reported the detection of SV40 nucleotide sequences in human tumors, mainly mesotheliomas, but the reports have not been consistent. The presence of SV40 in 26 consecutive cases of malignant mesothelioma of biphasic type was investigated using a SV40 quantitative real time polymerase chain reaction (PCR) with a sensitivity of 10 copies of viral DNA per sample. All the samples were also tested for amplifiability using a real-time PCR for the beta-globin gene. Eighteen tumors were amplifiable, but none contained SV40 DNA. The results do not support an association between mesothelioma and SV40.
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| 2. |
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| 3. |
- Lind, Alexander, et al.
(författare)
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First trimester enterovirus IgM and beta cell autoantibodies in mothers to children affected by type 1 diabetes autoimmunity before 7 years of age
- 2018
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Ingår i: Journal of Reproductive Immunology. - : Elsevier BV. - 0165-0378. ; 127, s. 1-6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Autoimmune (type 1) diabetes (T1D) is a frequent chronic disease in children and adolescents globally. Gestational enterovirus (EV) infections have been associated with an increased risk for T1D in the offspring. We test the hypothesis that EV infections during the first trimester were associated with beta cell autoantibodies in mothers of children who developed islet autoantibodies before 7 years of age. Materials and methods: Local registries were used to identify mothers to children born 2000–2007 who developed either beta cell autoantibodies or T1D during follow up. Serum samples from the first trimester were located in the Biobank. A total of 448 index mothers were identified and compared to 891 matched control mothers. EV-IgM was determined in a capture enzyme immunoassay. Beta cell autoantibodies were analyzed in standard radio binding assays. Results: The frequency of EV-IgM in index mothers was 20% (89/448), which did not differ from the control mothers 20% (175/891) (p = 0.922). Index mothers had multiple beta cell autoantibodies more often than control mothers (p = 0.037). Beta cell autoantibodies were increased during the November–April winter months in index compared to control mothers (p = 0.022). The observed difference was possibly explained by the months of February-April (p = 0.014). Concomitant EV-IgM and beta cell autoantibodies tended to be more common among index compared to control mothers (p = 0.039). Conclusion: EV-IgM during the first trimester may be associated with beta cell autoantibodies in mothers to children who developed either beta cell autoantibodies or T1D before 7 years of age.
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| 4. |
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| 5. |
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| 6. |
- Lundstig, Annika, et al.
(författare)
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Neutralizing Ljungan virus antibodies in children with newly diagnosed type 1 diabetes
- 2021
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Ingår i: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 102:5
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Tidskriftsartikel (refereegranskat)abstract
- Ljungan virus (LV), a Parechovirus of the Picornavirus family, first isolated from a bank vole at the Ljungan river in Sweden, has been implicated in the risk for autoimmune type 1 diabetes. An assay for neutralizing Ljungan virus antibodies (NLVA) was developed using the original 87-012 LV isolate. The goal was to determine NLVA titres in incident 0-18 years old newly diagnosed type 1 diabetes patients (n=67) and school children controls (n=292) from Jämtland county in Sweden. NLVA were found in 41 of 67 (61 %) patients compared to 127 of 292 (44 %) controls (P=0.009). In the type 1 diabetes patients, NLVA titres were associated with autoantibodies to glutamic acid decarboxylase (GADA) (P=0.023), but not to autoantibodies against insulin (IAA) or islet antigen-2 (IA-2A). The NLVA assay should prove useful for further investigations to determine levels of LV antibodies in patients and future studies to determine a possible role of LV in autoimmune type 1 diabetes.
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| 7. |
- Lundstig, Annika, et al.
(författare)
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Neutralizing Ljungan virus antibodies in children with newly diagnosed type 1 diabetes
- 2021
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Ingår i: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 102:5
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Tidskriftsartikel (refereegranskat)abstract
- Ljungan virus (LV), a Parechovirus of the Picornavirus family, first isolated from a bank vole at the Ljungan river in Sweden, has been implicated in the risk for autoimmune type 1 diabetes. An assay for neutralizing Ljungan virus antibodies (NLVA) was developed using the original 87-012 LV isolate. The goal was to determine NLVA titres in incident 0-18 years old newly diagnosed type 1 diabetes patients (n=67) and school children controls (n=292) from Jämtland county in Sweden. NLVA were found in 41 of 67 (61 %) patients compared to 127 of 292 (44 %) controls (P=0.009). In the type 1 diabetes patients, NLVA titres were associated with autoantibodies to glutamic acid decarboxylase (GADA) (P=0.023), but not to autoantibodies against insulin (IAA) or islet antigen-2 (IA-2A). The NLVA assay should prove useful for further investigations to determine levels of LV antibodies in patients and future studies to determine a possible role of LV in autoimmune type 1 diabetes.
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| 8. |
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| 9. |
- Lundstig, Annika
(författare)
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Polyomavirus infections in humans
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The human polyomaviruses BKV and JCV are endemic and infect > 70% of population worldwide. Primary infections occur during childhood and are largely subclinical. Following primary infection, both viruses persist as latent infections in the kidneys and B lymphocytes. Under conditions of severe immunosuppression like leukaemia, organ transplantation and AIDS, the viruses can reactivate and cause diseases. BKV is mainly related to urinary tract diseases and JCV is the causative agent of progressive multifocal leukoencephalopathy (PML). The human polyomaviruses have oncogenic potential and a possible association of with human cancer has been reported. JCV has been detected in certain brain tumours, in particular oligoastrocytoma and BKV has been detected in a variety of tumours, including neuroblastoma. Simian virus 40 (SV40), of rhesus monkey origin was accidentally introduced to humans through contaminated polio vaccine. Several studies have detected SV40 sequences in human tumours, mainly mesothelioma, osteosarcoma, ependymomas and choroid plexus tumours. The aim of the thesis was to study the infections of polyomaviruses in humans and their role in human cancers. We have established a VLP-based EIA BKV, JCV and SV40. Sera from Swedish children showed that BKV and JCV increased by age. Seropositivity was generally stable over time in serial samples. Analysis of maternal sera using both serology and DNA detection, found no evidence for association between BKV or JCV infection during pregnancy and an increased risk of developing neuroblastoma in the child. Sera from 386 cases of colorectal cancer and controls were investigated for JCV and BKV IgG seropositivity. The serologic assay used was validated within the study and found to have very high sensitivity for detecting subjects with polyoma virus shedding. Our study found no evidence for association between infection with the human polyomaviruses and excess risk for colorectal cancer. A low prevalence (7.6%) of SV40-specific antibodies was detected in the Nordic population. None of the SV40-seropositive samples contained detectable SV40 DNA. The investigation of 28 malignant mesothelioma tissues from deceased patients in Sweden found no detectable SV40 DNA. In summary, modern assays to detect polyomavirus antibodies and DNA has been established. We have now knowledge of the presence and age-specific prevalence of BKV, JCV and SV40 in Nordic countries. We did not confirm any association between infection with polyomaviruses and cancer.
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| 10. |
- Lundstig, Annika, et al.
(författare)
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Prevalence and stability of human serum antibodies to simian virus 40 VP1 virus-like particles.
- 2005
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Ingår i: Journal of General Virology. - : Microbiology Society. - 1465-2099 .- 0022-1317. ; 86:Pt 6, s. 1703-1708
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Tidskriftsartikel (refereegranskat)abstract
- Possible human infection with simian virus 40 (SV40) has been of great concern ever since SV40 was discovered in polio vaccines. Human populations are SV40-seropositive, but because of serological cross-reactivity between SV40 and the human polyomaviruses BK virus (BKV) and JC virus (JCV), it is debatable whether these antibodies are specific. An SV40-specific serological assay was established, based on purified virus-like particles (VLPs), where the SV40 VLPs were blocked with hyperimmune sera to BKV and JCV. Competition with SV40 hyperimmune sera was used as a confirmatory test. Among 288 Swedish children of between 1 and 13 years of age, 7·6 % had SV40-specific antibodies. SV40 seroprevalence reached a peak of 14 % at 7–9 years of age. Among 100 control patients with benign tumours, 9 % were SV40-seropositive. However, SV40 DNA was not detectable in corresponding buffy-coat samples. In serial samples taken up to 5 years apart from 141 Finnish women participating in the population-based serological screening for congenital infections, only two of 141 women were SV40-seropositive in both samples. Six women seroconverted and eight women had a loss of antibodies over time. None of the SV40-seropositive samples contained detectable SV40 DNA. In conclusion, there is a low prevalence of SV40-specific antibodies in the Nordic population. The SV40 antibodies appear to have a low stability over time and their origin is not clear.
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