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- Båge, Renee, et al.
(författare)
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Characterization of coagulase negative staphylococci from cases of subclinical mastitis in dairy cattle in Kampala, Uganda
- 2014
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Ingår i: Irish Veterinary Journal. - : Springer Science and Business Media LLC. - 2046-0481. ; 67
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Tidskriftsartikel (refereegranskat)abstract
- Background: Coagulase negative staphylococci (CNS) are the most common pathogens leading to subclinical mastitis (SCM) in dairy cattle in Uganda. Coagulase negative staphylococci can vary between bacterial species in how they cause disease. The aim of the study was to characterize CNS, from cows with SCM in Uganda, at the species level. Findings: Quarter milk samples (n = 166) were collected from 78 animals with SCM. Bacteriological analyses were carried out at Makerere University, Kampala, Uganda and at the National Veterinary Institute (SVA), Uppsala, Sweden. The most common pathogens found in milk samples from cows with SCM were CNS (31.7%). Two species of CNS were found, S. epidermidis (85%) and S. haemolyticus (15%). Of the CNS isolates, 16/20 (80%) were positive for β-lactamase production (β+). Conclusions: In milk samples from cows with SCM caused by CNS, S. epidermidis was most prevalent, followed by S. haemolyticus
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| 5. |
- Ikwap, Kokas, et al.
(författare)
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Characterization of pig production in Gulu and Soroti districts in northern and eastern Uganda
- 2014
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Ingår i: Livestock Research For Rural Development. - 0121-3784. ; 26
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Tidskriftsartikel (refereegranskat)abstract
- Owing to loss of cattle and goats during the recent civil unrest, pig farming has become popular in northern and eastern Uganda as a quick mitigation to poverty. This study was carried out to describe the characteristics of pig production in these regions. Using a cross-sectional design, data were collected from 96 households (51 from Gulu district, northern Uganda and 45 from Soroti district, eastern Uganda) raising pigs with suckling and weaned piglets. The households were selected using the snowballing method. The households were predominantly headed by adult men, of which 97% had attended at least primary education. The mean numbers of suckling, weaned, growing and adult pigs per household in Gulu and Soroti were 8 and 7.3, 4.8 and 5.3, 2.3 and 2.6 and 3.1 and 3.1, respectively, with no significant difference (p > 0.05) between the districts. Overall, the majority of households (64%, n=96) kept local breeds of pigs. The most common management method was tethering, as practiced by 67% of the study households. Home-made feeds were the most common (88%, n=96) and the wives/women provided much of the labour (60%, n=96). However, in only 23% of the households, women owned and made decisions on the pigs. Male children also owned and made decisions on pigs in 8% of the households. Natural breeding of sows with a shared boar was the most common practice (88%, n=96). The litter size at birth was 6 to 10 piglets in 78% (n= 96) of the households. Based on the clinical signs reported by the households, 38% and 23% of the herds experienced problems with diarrhoea and respiratory diseases, respectively. In total, 39% of the households were receiving professional veterinary care, when pigs fall sick. In conclusion, pig production around regional urban centers in northern and eastern Uganda is largely smallholder, practiced by farmers who have attended at least primary education, tether their pigs, depend on labour provided largely by housewives and there is inadequate veterinary care. The findings of this study point at a need for increased involvement of women in decision making in pig farming, increased pig veterinary care and investigation of the causes of diseases such as diarrhoea in order to support this major livelihood resource for the poor in northern and eastern Uganda, especially women and children.
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| 7. |
- Ikwap, Kokas, et al.
(författare)
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Prevalence of adhesin and toxin genes in E-coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda
- 2016
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Ingår i: BMC Microbiology. - : Springer Science and Business Media LLC. - 1471-2180. ; 16
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Tidskriftsartikel (refereegranskat)abstract
- Background: Enterotoxigenic E. coli (ETEC) significantly contribute to diarrhea in piglets and weaners. The smallholder pig producers in Uganda identified diarrhea as one of the major problems especially in piglets. The aim of this study was to; i) characterize the virulence factors of E. coli strains isolated from diarrheic and non-diarrheic suckling piglets and weaners from smallholder herds in northern and eastern Uganda and ii) identify and describe the post-mortem picture of ETEC infection in severely diarrheic piglets. Rectal swab samples were collected from 83 piglets and weaners in 20 herds and isolated E. coli were characterized by PCR, serotyping and hemolysis.Results: The E. coli strains carried genes for the heat stable toxins STa, STb and EAST1 and adhesins F4 and AIDA-I. The genes for the heat labile toxin LT and adhesins F5, F6, F18 and F41 were not detected in any of the E. coli isolates. Where the serogroup could be identified, E. coli isolates from the same diarrheic pig belonged to the same serogroup. The prevalence of EAST1, STb, Stx2e, STa, AIDA-I, and F4 in the E. coli isolates from suckling piglets and weaners (diarrheic and non-diarrheic combined) was 29, 26.5, 2.4, 1.2, 16, and 8.4 %, respectively. However the prevalence of F4 and AIDA-I in E. coli from diarrheic suckling piglets alone was 22.2 and 20 %, respectively. There was no significant difference in the prevalence of the individual virulence factors in E. coli from the diarrheic and non-diarrheic pigs (p > 0.05). The main ETEC strains isolated from diarrheic and non-diarrheic pigs included F4/STb/EAST1 (7.2 %), F4/STb (1.2 %), AIDA/STb/EAST1 (8 %) and AIDA/STb (8 %). At post-mortem, two diarrheic suckling piglets carrying ETEC showed intact intestinal villi, enterocytes and brush border but with a layer of cells attached to the brush border, suggestive of ETEC infections.Conclusion: This study has shown that the F4 fimbriae is the most predominant in E. coli from diarrheic piglets in the study area and therefore an F4-based vaccine should be considered one of the preventive measures for controlling ETEC infections in the piglets in northern and eastern Uganda.
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| 8. |
- Ikwap, Kokas, et al.
(författare)
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Salmonella species in piglets and weaners from Uganda: Prevalence, antimicrobial resistance and herd-level risk factors
- 2014
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Ingår i: Preventive Veterinary Medicine. - : Elsevier BV. - 0167-5877 .- 1873-1716. ; 115, s. 39-47
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Tidskriftsartikel (refereegranskat)abstract
- Non-typhoidal salmonellosis is of concern in humans in sub-Saharan Africa, and this is partly due to the high number of immunocompromised persons. Pork and pork products could be among the sources of these non-typhi Salmonella spp. The aim of this study was to identify Salmonella spp. in piglets and weaners in northern and eastern Uganda, characterize their antimicrobial resistance patterns and determine herd-level risk factors. Fecal samples were collected from 465 piglets and weaners from 93 herds (49 and 44 from northern and eastern Uganda, respectively). In addition, information about the herd management and potential risk factors were collected. The fecal samples were cultured for the identification of Salmonella spp. The Salmonella spp. confirmed by serotyping were further characterized by determination of minimum inhibitory concentration (MIC) to 12 antimicrobials by broth microdilution. At individual level, the total prevalence of Salmonella spp. was 12% (12.2% in northern and 11.9% in eastern Uganda). At herd level, the total prevalence was 39% (43% in northern and 34% in eastern Uganda). From 56 samples with Salmonella spp., 20 serovars were identified including two serovars identified only by their antigenic formulae. The predominant serovars were S. Zanzibar, S. Heidelberg, S. Infantis, S. Typhimurium, S. Stanleyville, S. Aberdeen and S. Kampala. In total, 57% of the 53 Salmonella spp. analyzed, originating from 27% of the herds, were resistant to at least one antimicrobial agent. The majority of drug-resistant isolates (60%) were from northern Uganda. Eight multidrug-resistant (MDR) isolates were from northern Uganda and three MDR isolates were from eastern Uganda. Increased prevalence of Salmonella spp. was associated with feeding the young and adults separately as compared to feeding the young and adults together (p = 0.043, OR = 4.3; 95% CI 1.1, 17.38). Protective factors were "intensive" method of keeping the pigs versus "tethering and roaming" (p = 0.016, OR = 0.11; 95% CI 0.02, 0.64), "intensive" method versus "semi-intensive" method (p = 0.048, OR = 0.12; 95% CI 0.01, 0.96) and cleaning feeders after every two days versus daily (p = 0.017, OR = 0.18; 95% CI 0.05, 0.72). This study has revealed a high prevalence of infection of piglets and weaners with diverse non-typhi Salmonella serovars and highlights the potential role of pork and pork products as sources of these organisms for humans. In addition, this study has identified protective factors that could be promoted to control Salmonella spp. and in antimicrobial resistance reduction programs in rural pigs from Uganda. (C) 2014 Elsevier B.V. All rights reserved.
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| 9. |
- Mbabazi Kanyima, Benon, et al.
(författare)
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Husbandry Factors and the Resumption of Luteal Activity in Open and Zero-Grazed Dairy Cows in Urban and Peri-Urban Kampala, Uganda
- 2014
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Ingår i: Reproduction in Domestic Animals. - : Wiley. - 0936-6768 .- 1439-0531. ; 49, s. 673-678
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Tidskriftsartikel (refereegranskat)abstract
- The study investigated the influence of selected husbandry factors on interval to resumption of post-partum cyclicity among dairy cows in urban and peri-urban Kampala. A prospective study of 85 day post-partum period of 59 dairy cows in open (n = 38) and zero grazing (n = 21) systems was conducted on 24 farms. Cows of parity 1-6 were recruited starting 15-30 days post-partum. Progesterone (P4) content in milk taken at 10-12 day intervals was analysed using ELISA. The cow P4 profiles were classified into 'normal' (< 56 days), 'delayed' (> 56 days), 'ceased' or 'prolonged' (if started < 56 days but with abnormal P4 displays) resumption of luteal activity and tested for association with husbandry and cow factors. Of the 59 cows, luteal activity in 81.4% resumed normally and in 18.6%, delayed. Only 23.7% maintained regular luteal activity, while the others had ceased (10.2%), prolonged (37.3%) or unclear luteal activity (20.3%). There were no differences between open and zero-grazed cows. Milk production was higher (p < 0.05) in zero than open grazing, in urban than peri-urban and in cows fed on brew waste (p < 0.001) compared with mill products and banana peels. Results suggest that luteal activity resumes normally in a majority of cows, although only a minority experienced continued normal cyclicity once ovulation had occurred, in the two farming systems irrespective of feed supplements or water, and that supplementing with brew waste is beneficial for milk production.
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| 10. |
- Mbabazi Kanyima, Benon, et al.
(författare)
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Managerial practices and factors influencing reproductive performance of dairy cows in urban/peri-urban areas of Kampala and Gulu, Uganda
- 2015
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Ingår i: Acta Veterinaria Scandinavica. - : Springer Science and Business Media LLC. - 0044-605X .- 1751-0147. ; 57
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Tidskriftsartikel (refereegranskat)abstract
- Background: Urban/peri-urban dairy production and sales has evolved as an adjustment to cope with food security and economic needs for urban dwellers in low-income countries and created an opportunity to transform from subsistence rural lifestyles of dairy farming to commercial engagement in towns. However, urban/peri-urban dairy farms differ in challenges from rural dairy farms and reproduction is important and critical for assuring sustainable economic output in both environments. Here we recorded for the first time differences between two geographically and economically different cities corresponding to different settings within the same country in managerial factors influencing reproductive performance in urban/peri-urban dairy cowherds.Results: The urban/peri-urban dairy farmers in the capital Kampala were more often male (P = 0.002) and commercialized (P = 0.0025), more experienced (P = 0.0001) and practiced zero-grazing more often (P = 0.05) than in the regional municipality Gulu. Also, the milk production per herd and cow (P = 0.0005) and calving rate were (P = 0.0001) higher in Kampala and artificial insemination was more commonly (P = 0.002) used than in Gulu. There was no difference in abortion nor neonatal mortality rate between the two locations. Overall, calving rates were higher (P = 0.0003) in smaller (= 3 dairy cows) and open grazing (P = 0.003) herds. Abortion rates were higher among dairy herds practicing late (= 5 months) (P = 0.003) calf weaning and in herds with commercial purposes (P = 0.0001). Neonatal calf mortality was lower (P = 0.01) in small herds.Conclusion: The study showed significant differences between Kampala and Gulu in reproductive performance and related husbandry factors for cows in the urban/peri-urban dairy farming systems. For several reproductive performance traits we found associations with husbandry and production traits, which should be taken into account when providing advice to the urban and peri-urban dairy farmers in the tropics.
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