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- Kaur-Kahlon, G., et al.
(författare)
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Response of a coastal tropical pelagic microbial community to changed salinity and temperature
- 2016
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Ingår i: Aquatic Microbial Ecology. - 0948-3055 .- 1616-1564. ; 77:1, s. 37-50
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Tidskriftsartikel (refereegranskat)abstract
- Studies on the responses of tropical microbial communities to changing hydrographic conditions are presently poorly represented. We present here the results from a mesocosm experiment conducted in southwest (SW) coastal India to investigate how changes in temperature and salinity may affect a coastal tropic microbial community. The onset of algal and bacterial blooms, the maximum production and biomass, and the interrelation between phytoplankton and bacteria were studied in replicated mesocosms. The treatments were set up featuring ambient conditions (28 °C, 35 PSU), hyposalinity (31 PSU), warming (31 °C) and a double manipulated treatment with warming and hyposalinity (31 °C, 31 PSU). The hyposaline treatment had the most considerable influence manifested as significantly lower primary production, and the most dissimilar microphytoplankton species community. The increased temperature acted as a catalyst in the double manipulated treatment and higher primary production was maintained. We investigated the dynamics of the microbial community with a structural equation model approach, and found a significant interrelation between phytoplankton biomass and bacterial abundance. Using this methodology, it became evident that temperature and salinity changes, individually and together, mediate direct and indirect effects that influence different compartments of the microbial loop. In the face of climate change, we suggest that in relatively nutrient replete tropical coastal zones, salinity and temperature changes will affect nutrient assimilation with subsequent significant effects on the quantity of microbial biomass and production.
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| 2. |
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Bioblitz i Arkelstorp 16-17 augusti 2019
- 2020
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- Arkelstorpsviken är den nordvästra delen av Oppmannasjön, som ären av Skånes största sjöar. Idén att genomföra en så kallad Bioblitzvid Arkelstorpsviken föddes under ett styrelsemöte i projektet "En viki Sjöriket Skåne" som är ett samarbete mellan Oppmanna Vånga Bygderåd och Högskolan Kristianstad. Projektets främsta syfte är att hitta en lösning på den kraftiga övergödningen i Arkelstorpsviken. Detta är ett ”Leader”-finansierat projekt, vilket innebär att stommen i projektetär lokal förankring. Det fanns röster i byn som kände att man gav området onödigt dåligt rykte genom att ständigt lyfta fram problemen med vattenstatusen i sjön. Under ett styrelsemöte 30 sep 2018 föddes iden att genom en Bioblitz lyfta fram de positiva värdena i och kring sjön. Den naturliga samarbetspartnern för detta projekt var forskningsmiljön MABH (Man & Biosphere Health) vid Högskolan Kristianstad,vars medlemmar tillsammans besitter en mycket bred biologisk kunskap.Med MABH i ryggen var alltså kompetensen säkrad för att genomföra en Bioblitz. Inbjudningar skickades ut till lokala naturorganisationerför att hitta ännu fler experter som kunde hjälpa till med särskilda artgrupper. Samtidigt jobbade man aktivt lokalt med att försöka engagera intresserad allmänhet. Inbjudningar och direktreklam skickades ut till samtliga hushåll med postadress Arkelstorp. I ett försök att synas genom mediebruset anordnades en tävling, som gick ut på att gissa antalet arter (taxa) som hittades under Bioblitzen. Två lokala företag ställde upp och första priset för den vuxna individ som gissade närmstvar en 3-rätters måltid på Bäckaskogs Slott. De yngre tävlande kunde vinna en kanotutflykt med familjen på Ivögården.
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| 5. |
- Asplund, Maria E., et al.
(författare)
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Water column dynamics of Vibrio in relation to phytoplankton community composition and environmental conditions in a tropical coastal area
- 2011
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Ingår i: Environmental Microbiology. - 1462-2912 .- 1462-2920. ; 13:10, s. 2738-2751
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Tidskriftsartikel (refereegranskat)abstract
- Vibrio abundance generally displays seasonal patterns. In temperate coastal areas, temperature and salinity influence Vibrio growth, whereas in tropical areas this pattern is not obvious. The present study assessed the dynamics of Vibrio in the Arabian Sea, 1-2 km off Mangalore on the south-west coast of India, during temporally separated periods. The two sampling periods were signified by oligotrophic conditions, and stable temperatures and salinity. Vibrio abundance was estimated by culture-independent techniques in relation to phytoplankton community composition and environmental variables. The results showed that the Vibrio density during December 2007 was 10- to 100-fold higher compared with the February-March 2008 period. High Vibrio abundance in December coincided with a diatom-dominated phytoplankton assemblage. A partial least squares (PLS) regression model indicated that diatom biomass was the primary predictor variable. Low nutrient levels suggested high water column turnover rate, which bacteria compensated for by using organic molecules leaking from phytoplankton. The abundance of potential Vibrio predators was low during both sampling periods; therefore it is suggested that resource supply from primary producers is more important than top-down control by predators.
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| 7. |
- Axelsson, Carolina, 1967-, et al.
(författare)
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Optimization of several parameters in order to reduce time in antibiotic susceptibility testing in a clinical laboratory
- 2017
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background - When sepsis or bacteraemia is suspected, patient blood samples are cultivated in blood culture bottles and then further incubated for identification of the organism and antimicrobial susceptibility testing. These methods are slow, identifying causative pathogens in a couple of hours, and antibiotic susceptibility results within 18-36 hours.Here we present optimization of several parameters in order to evaluate if the MBT ASTRA™ method can be a rapid tool, used for routine antibiotics susceptibility testing, in a clinical laboratory.Methods – MALDI-TOF MS measurements were performed with a Microflex LT/SH bench-top mass spectrometer (Bruker) with standard settings. The resulting spectra were uploaded in the MBT-ASTRA™ software, which normalizes the peaks and determines the AUC and RG values for each setup.Results - The bacterial preparation steps generated a new protocol, which reduced time with 30-60 minutes.The antibiotics susceptibility test was optimized for 90 minutes incubation time. 200 µl McFarland 0.5 bacterial suspension in broth were incubated in broth at 37°C, with and without 32 µg/ml Cefotaxime, 16 µg/ml Meropenem and 4 µg/ml Ciprofloxacin.The suspensions were transferred to 0.45 µm pore size filter membraned 96 well plate. They were centrifuged; washed; fixated and eluted; put on a MALDI-target, and covered by matrix solution. All could be automated with robot, which reduced time with 60 minutes.Conclusion – Rapid susceptibility testing becomes more requested with the increase of resistance bacteria causing infections. Our study can be a valuable tool for clinical laboratories striving for reduction in time handling of antibiotic susceptibility testing.
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| 8. |
- Collin, Betty, 1976, et al.
(författare)
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Characteristics of potentially pathogenic vibrios from subtropical Mozambique compared with isolates from tropical India and boreal Sweden
- 2013
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Ingår i: Fems Microbiology Ecology. - : Oxford University Press (OUP). - 0168-6496 .- 1574-6941. ; 83:2, s. 255-264
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Tidskriftsartikel (refereegranskat)abstract
- Reported outbreaks of Vibrio parahaemolyticus have increased worldwide, particularly in regions of high seafood consumption. In Mozambique, seafood constitutes an important food resource and diarrheal diseases are common among its inhabitants. Edible clams were collected in Maputo Bay during both the dry and rainy seasons, with the results showing the number of viable counts of vibrios in clams to peak during the latter. Vibrio parahaemolyticus was the predominant species identified among the isolated strains. Although only one of 109 total strains carried the tdh virulence gene, 69% of isolates showed evidence of hemolytic capacity when subjected to a functional test. Similar virulence patterns and biochemical properties were found in strains isolated from Indian and Swedish marine waters. Antibiotic resistance was, however, more pronounced in strains isolated from these latter two environments.
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| 9. |
- Collin, Betty, 1976-, et al.
(författare)
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Faecal contaminants in edible bivalves from Maputo Bay, Mozambique : seasonal distribution, pathogenesis and antibiotic resistance
- 2008
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Ingår i: Open Nutrition Journal. - 1874-2882. ; 2, s. 86-93
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Tidskriftsartikel (refereegranskat)abstract
- In Maputo, Mozambique marine bivalves considerably contribute to the diet of the population. This study aimed to investigate seasonal distribution of Escherichia coli and Salmonella in clams from Maputo Bay, and examine their pathogenesis and antibiotic-resistant patterns. Standard multiple tube method revealed that the concentration of coliforms in all samples exceeded the limit for direct consumption, according to EU standards. Thirty-eight percent of the samples contained >60,000 MPN per 100 gram flesh. The occurrence of E. coli did not differ significantly due to season, while Salmonella was present in 100% of the samples during the rainy period and only in 30% during the dry. Multiplex polymerase chain reaction showed that 45% of E. coli isolates were positive for the virulent indicator gene fimA. The Salmonella isolates were identified as S. enterica serovar Typhimurium. Among other isolated coliformic Enterobacteriaceae, Shigella sp. (specie), which in low doses can cause severe gastrointestinal infections, was identified. Antimicrobial susceptibility, recorded by the disk diffusion method, showed resistance to the most commonly used antibiotics. This high levels of faecal contaminants in the clams points out the need for risk assessment and sanitary improvements.
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| 10. |
- Collin, Betty, 1976, et al.
(författare)
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Faecal Contaminants in Edible Bivalves from Maputo Bay, Mozambique
- 2008
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Ingår i: The Open Nutrition Journal. - 1874-2882. ; 2, s. 86-93
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Tidskriftsartikel (refereegranskat)abstract
- In Maputo, Mozambique marine bivalves considerably contribute to the diet of the population. This study aimed to investigate seasonal distribution of Escherichia coli and Salmonella in clams from Maputo Bay, and examine their pathogenesis and antibiotic-resistant patterns. Standard multiple tube method revealed that the concentration of coliforms in all samples exceeded the limit for direct consumption, according to EU standards. Thirty-eight percent of the samples contained >60,000 MPN per 100 gram flesh. The occurrence of E. coli did not differ significantly due to season, while Salmonella was present in 100% of the samples during the rainy period and only in 30% during the dry. Multiplex polymerase chain reaction showed that 45% of E. coli isolates were positive for the virulent indicator gene fimA. The Salmonella isolates were identified as S. enterica serovar Typhimurium. Among other isolated coliformic Enterobacteriaceae, Shigella sp. (specie), which in low doses can cause severe gastrointestinal infections, was identified. Antimicrobial susceptibility, recorded by the disk diffusion method, showed resistance to the most commonly used antibiotics. This high levels of faecal contaminants in the clams points out the need for risk assessment and sanitary improvements.
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