| 1. |
- Widén, Cecilia, et al.
(författare)
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Erythrocyte antioxidant protection of rose hips (Rosa spp.)
- 2012
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Ingår i: Oxidative Medicine and Cellular Longevity. - : Landes Bioscience / Hindawi Publishing Corporation. - 1942-0900 .- 1942-0994. ; , s. 621579-
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Tidskriftsartikel (refereegranskat)abstract
- Rose hips are popular in health promoting products as the fruits contain high content of bioactive compounds. The aim of this study was to investigate whether health benefits are attributable to ascorbic acid, phenols, or other rose-hip-derived compounds. Freeze-dried powder of rose hips was preextracted with metaphosphoric acid and the sample was then sequentially eluted on a C18 column. The degree of amelioration of oxidative damage was determined in an erythrocyte in vitro bioassay by comparing the effects of a reducing agent on erythrocytes alone or on erythrocytes pretreated with berry extracts. The maximum protection against oxidative stress, 59.4±4.0% (mean ± standard deviation), was achieved when incubating the cells with the first eluted meta-phosphoric extract. Removal of ascorbic acid from this extract increased the protection against oxidative stress to 67.9±1.9%. The protection from the 20% and 100% methanol extracts was 20.8±8.2% and 5.0±3.2%, respectively. Antioxidant uptake was confirmed by measurement of catechin by HPLC-ESI-MS in the 20% methanol extract. The fact that all sequentially eluted extracts studied contributed to protective effects on the erythrocytes indicates that rose hips contain a promising level of clinically relevant antioxidant protection.
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| 2. |
- Widén, Cecilia, et al.
(författare)
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Protection of human erythrocytes against oxidative stress by berries
- 2012
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Ingår i: Journal of Berry Research. - : IOS Press. - 1878-5093 .- 1878-5123. ; 2:3
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Tidskriftsartikel (refereegranskat)abstract
- Berries contain several bioactive compounds that can protect against oxidative stress. In this study we evaluated the protective effect of different sequential extracts (ethyl acetate, ethanol and water) of seven berry species: bilberry (Vaccinium myrtillus), blackcurrant (Ribes nigrum), elderberry (Sambucus nigra), lingonberry (Vaccinium vitis-idaea), rose hips (Rosa sp.), sea buckthorn (Hippohae rhamnoides) and strawberry (Fragaria × ananassa). The protective effect was tested on human erythrocytes and the antioxidant capacity was also evaluated in vitro by the FRAP assay. In the erythrocyte assay all sea buckthorn extracts were superior in antioxidant effect to other berry extracts. The ethyl acetate extract of bilberries, and the ethanol and water extracts of blackcurrants, also protected the erythrocytes from oxidation. In contrast, water extracts of rose hips, bilberries and strawberries had a pro-oxidant effect on erythrocytes. The water extract of rose hips was superior to the other berry extracts in the FRAP assay. Thus, the results of the erythrocyte assay did not correlate with the results of the FRAP assay, but provided additional insights into the potential protective effects of berry extracts against oxidative stress.
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| 3. |
- Ahmadi Afzadi, Masoud, et al.
(författare)
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Biochemical contents of apple peel and flesh affect level of partial resistance to blue mold
- 2015
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Ingår i: Postharvest Biology and Technology. - : Elsevier BV. - 0925-5214. ; 110, s. 173-182
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Tidskriftsartikel (refereegranskat)abstract
- Apple fruit contains a wide range of chemical compounds that may contribute to resistance against blue mold caused by Penicillium expansum. In the present study, contents of total titratable acidity, malic acid, total phenols and 10 individual phenolic compounds were quantified in peel and flesh fractions of both control and blue mold-inoculated fruits of 24 apple cultivars. In addition to the significant variation among cultivars in terms of all quantified compounds, correlation analysis revealed a significant impact of total phenols and individual phenols like flavonols and procyanidins B2 in the peel fraction, on blue mold resistance in the inoculated fruits. Multivariate analyses on data for chemical compounds in peel tissue of inoculated fruits, could also separate resistant and susceptible cultivars. These findings can be useful in breeding programs since higher levels of phenolic compounds may indicate better resistance in apple cultivars. (C) 2015 Elsevier B.V. All rights reserved.
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| 4. |
- Ahmadi Afzadi, Masoud, et al.
(författare)
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Genetics of resistance to blue mould in apple: inoculation-based screening, transcriptomics and biochemistry
- 2016
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Ingår i: Acta Horticulturae. - 0567-7572 .- 2406-6168. ; 1127, s. 55-60
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Konferensbidrag (refereegranskat)abstract
- Blue mould caused by Penicillium expansum is a major contributor to loss of marketable fruit during storage. Damage is also aggravated by the prohibition of postharvest fungicides in many countries, and in organic production everywhere. Robust data on levels of resistance to this disease are crucial for further research and for breeding of improved cultivars. Inoculation of fruit with fungal spores and estimation of the diameter of resulting lesions after cold storage demonstrated significant variation among cultivars. In addition, cultivars with high firmness at harvest and little softening, i.e., loss of firmness during storage, had less susceptibility, as also did late-ripening cultivars. In another part of the study, a possible association between lesion diameter and chemical contents in fruit flesh and fruit peel of 23 cultivars was investigated. Content of different quercetins and procyanidin B2 was positively associated with the level of tolerance. Samples taken after inoculation showed stronger association with the chemical contents than did samples of control fruit, suggesting that disease development had triggered a defence reaction. In a third part of the study, gene regulation in apple fruit after inoculation was evaluated in two relatively resistant and two relatively susceptible cultivars using an AryANE chip covering 60K apple transcripts. Validation of the most highly up-and down-regulated genes was undertaken using qPCR. Some genes related to disease resistance, biosynthesis of flavonoids and to cell-wall structure were identified as most likely to be responsible for differences in susceptibility to blue mould. These results will be used to search for candidate genes that can be used in marker-assisted selection in apple breeding.
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| 5. |
- Andersson, Staffan, et al.
(författare)
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Carotenoid content and composition in rose hips (Rosa spp.) during ripening, determination of suitable maturity marker and implications for health promoting food products
- 2011
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Ingår i: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 128, s. 689-696
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Tidskriftsartikel (refereegranskat)abstract
- Carotenoids are important antioxidants and valuable bioactive compounds contributing to the health benefits of different foods, and rose hips are known for high bioactive content. This study investigated the content of carotenoids and chlorophylls in hips from four rose species during the ripening period. Carotenoid content varied greatly between harvesting times, although species and year, and interactions between factors, also played an important role. Some carotenoids were identified only in hips of specific rose species. Some of the investigated compounds decreased (chlorophyll a) or increased (lycopene) consistently over the ripening period and could therefore be useful as maturity markers. An optimal content of total or specific carotenoids, e.g. for food products containing rose hips, might be obtained by proper choice of harvesting time and rose species. (C) 2011 Elsevier Ltd. All rights reserved.
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| 6. |
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| 7. |
- Andersson, Staffan, et al.
(författare)
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Effect of storage time and temperature on stability of bioactive compounds in aseptically packed beverages prepared from rose hips and sea buckthorn berries
- 2015
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Ingår i: Agricultural and Food Science. - 1795-1895 .- 1459-6067. ; 24:4, s. 273-288
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Tidskriftsartikel (refereegranskat)abstract
- Rose hips and sea buckthorn berries contain high amounts of beneficial bioactive compounds. In this study the content and stability of natural vitamins and antioxidants were investigated in two fruit beverages made from rose hip powder, sea buckthorn berry puree and white grape juice concentrate, and wheat germ oil in one of the beverages. Beverages were formulated, pasteurised and filled aseptically in Tetra Brik packages and stored up to 35 days at 4 degrees C or 22 degrees C. Samples were analysed for the content of ascorbic acid, carotenoids, major phenolics, tocochromanols, total phenols and total antioxidant capacity as ferric reducing ability. The largest changes occurred during the first 5 days of storage, thereafter mainly ascorbic acid decreased. In the wheat germ oil supplemented beverage, alpha-tocopherol was more rapidly degraded. Negative correlations between the decrease of some carotenoids and tocochromanols, and the increase of some phenolics, suggest relationships of redox reactions specific to the amount of oil supplemented. From the present study we can conclude that additions of oil to beverages should be carefully selected to avoid oxidations of bioactive compounds. Furthermore, packaging of the beverages should be carried out at higher temperatures and thereafter it should be stored at low temperatures (4 degrees C), which decreased oxidation of all bioactive compounds except ascorbic acid.
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| 8. |
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| 9. |
- Andersson, Staffan, et al.
(författare)
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Tocopherols in rose hips (Rosa spp.) during ripening
- 2012
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Ingår i: Journal of the Science of Food and Agriculture. - : Wiley. - 0022-5142 .- 1097-0010. ; 92, s. 2116-2121
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Rose hips are used as a food ingredient and in health products. They are rich in various bioactive compounds such as carotenoids and vitamin C, but data on their vitamin E content (tocopherols and tocotrienols) are limited. In this study, four different species ofRosa were analysed for tocopherol and tocotrienol content during ripening in three different years. RESULTS: Only a- and ?-tocopherol were found in the fleshy parts of the rose hips, and the tocopherol content and vitamin E activity varied depending on date of harvesting, species and year. The amount of vitamin E activity differed between species of Rosa and years, whereas the changes during ripening were relatively small. CONCLUSION: The choice of species must be considered if tocopherol content is to be optimised when rose hips are used as a food ingredient. Copyright (C) 2012 Society of Chemical Industry
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| 10. |
- Burri, Stina C.M., et al.
(författare)
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Antioxidant capacity and major phenol compounds of horticultural plant materials not usually used
- 2017
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Ingår i: Journal of Functional Foods. - : Elsevier BV. - 1756-4646. ; 38, s. 119-127
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Tidskriftsartikel (refereegranskat)abstract
- Horticultural plant materials not usually used from onion, carrot, beetroot, sea buckthorn, black and red currants as well as a wastewater powder from olive oil production were analyzed for total phenols content (FC), ferric reducing ability of plasma (FRAP), radical scavenging capacity (ABTS), and for major phenolic compounds by HPLC-MS. Antioxidant capacity and phenol content varied significantly between species and cultivars, with extracts of sea buckthorn leaves being superior. In different species, different phenolic compounds were closely associated with FRAP, ABTS and FC. For instance, hydrolysable tannins were major antioxidants in sea buckthorn whereas quercetin was the major antioxidant in onion peel and skin. This study shows that horticultural plant materials usually left in the field or waste materials from processing may have high antioxidant properties, and that extracts of these materials therefore could be of potential interest for development of antioxidant food additives.
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