| 1. |
- Kowalik, S, et al.
(författare)
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Influence of alpha-ketoglutarate on bone mineral density of the femur in piglets
- 2005
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Ingår i: Bulletin of the Veterinary Institute in Puławy. - 0042-4870. ; 49:3, s. 343-348
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to determine the influence of daily oral administration of alpha-ketoglutarate (AKG) on bone mineral density of the femur and concentration of 17-beta-oestradiol in blood plasma during 70 d of postnatal life in piglets. All the animals were kept under standard rearing conditions. AKG was administered orally from the 1(st) d of life, while the control piglets were treated in the same way and time with physiological saline. The experimental and control groups were assigned to 6 age subgroups: 3, 14, 21, 35, 56 and 70 d of life. The animals from both groups were euthanised, then bone samples were collected and frozen at -25 degrees C until further analyses. Using dual-energy x-ray absorptiometry (DEXA method) bone mineral density of the femora was estimated. Additionally, 17-beta-oestradiol concentration in blood plasma was assayed using RIA-test. The obtained results indicate positive influence of enteral AKG administration on bone mineral density of the femur in piglets. Moreover, AKG increased the level of 17-beta-oestradiol in blood plasma in post-weaned piglets.
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| 2. |
- Sliwa, E, et al.
(författare)
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Effect of alpha-ketoglutarate given to pregnant sows on the development of the humerus and femur in newborns
- 2005
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Ingår i: Bulletin of the Veterinary Institute in Puławy. - 0042-4870. ; 49:1, s. 117-120
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Tidskriftsartikel (refereegranskat)abstract
- Alpha-ketoglutarate (AKG) was given to pregnant sows every day starting from the 91(st) d of pregnancy to farrowing. At the same time control pregnant sows were treated in the same way with physiological saline (PhS). Newborn piglets from the experimental (AKG) and control (PhS) mothers were euthanised in 1 h after their birth, and the femora and humeri were isolated and frozen at -25 degrees C until further analyses. Using quantitative computed tomography (QCT) method, volumetric bone density of the cortical and trabecular bone compartments were estimated. Moreover, geometrical and mechanical parameters of the bones and the level of osteocalcin and alkaline phosphatase activity in serum were determined. It was found that the administration of AKG to pregnant sows during 24 d before parturition decreased bone density in their newborns.
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| 3. |
- Sliwa, E, et al.
(författare)
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Influence of dexamethasone and alpha-ketoglutarate administration to pregnant sows on the level of some hormones in the blood plasma of newborn un-suckled piglets
- 2005
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 61:12, s. 1405-1407
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to establish the influence of dexamethasone and alpha-ketoglutarate administration to pregnant sows on the prenatal programming of the growth hormone level, cortisol, insulin and IGF-I in the blood serum of newborn piglets assessed just after their birth and prior to suckling. The foetal development is dependent on and determined by hormones such as glucocorticoids as well as nutrient supply through the sow, especially from the digestive tract. All these factors determine pre- and neonatal growth. The experiment was conducted over the final 24 days of pregnancy in sows treated i.m. with dexamethasone at a dose of 3 mg per sow every second day or alpha-ketoglutarate administered orally daily at a dose of 0.4 g/kg b.w. The blood samples were collected from the subclavian vein of un-suckled piglets immediately following their birth and were centrifuged straight away and the serum stored at -25 degrees C until further analysis. Hormone concentrations were determined using ELISA kit. The obtained results indicate that maternal administration of dexamethasone increased the level of cortisol while alpha-ketoglutarate increased the levels of cortisol and IGF I whereas simultaneous administration of dexamethasone and AKG significantly increased the growth hormone. These results of separate or simultaneous maternal administration of dexamethasone and alpha-ketogluta rate during the last 24 days of pregnancy indicated the activation of different mechanisms and different prenatal effects on the levels of cortisol, growth hormone and IGF I in serum assessed immediately following the birth of the piglets.
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| 4. |
- Sliwa, E, et al.
(författare)
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Influence of dexamethasone and alpha-ketoglutarate on growth and development of the skeletal system of pigs
- 2005
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 61:5, s. 526-531
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to establish the influence of dexamethasone and/or alpha-ketoglutarate (AKG) on the growth, mineralization, geometric and mechanical properties of ribs as a methodological model. The experiment was conducted during the final 24 days of prenatal life and first 14 days of neonatal life. The volumetric bone density of the trabecular and cortical bone was determined separately using the quantitative computed tomography (QCT) method and Somatom AR. T - Siemens apparatus. The mechanical properties were determined using the three-point bending test according to the method by Ferretti et al. The obtained results indicate that maternal administration of dexamethasone increased volumetric mineral density, although the mechanical parameters had lower values. Prenatal administration of AKG decreased all the geometric and mechanical parameters of the investigated ribs. The results obtained during the administration of the compound in neonatal life were the opposite of the prenatal results. Dexamethasone and AKG given together had the same influence on mineralization and other properties AND increasing their values during prenatal and neonatal life. This model of administrating dexamethasone and AKG maternally during prenatal life with the intention of provoking structural growth of the skeleton may be useful in further elucidating some biological and pharmacological factors on bone formation in prenatal life.
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| 5. |
- Tatara, MR, et al.
(författare)
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Effect of alpha-ketoglutarate (AKG) on mineralisation, morphology and mechanical endurance of femur and tibia in turkey
- 2004
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Ingår i: Bulletin of the Veterinary Institute in Puławy. - 0042-4870. ; 48:3, s. 305-309
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to determine the influence of alpha-ketoglutarate (AKG) on volumetric bone density, geometrical and mechanical properties of femur and tibia in farm turkeys. All animals were kept under standard rearing conditions with constant access to water and appropriate feed, supplied in accordance with the stage of production cycle. Bone samples were collected 14 weeks after beginning of the experiment. To the first group belonged control turkeys. The second group of animals consisted of turkeys that were administered AKG from the 22(nd) to 110(th) d of life. Using Quantitative Computed Tomography (QCT) method, volumetric bone density of femur and tibia were estimated for trabecular and cortical bone. Both the mechanical and geometrical properties of femur and tibia were assessed according to Ferretti's method. The obtained results indicate positive influence of enteral AKG administration on skeletal system quality in turkeys.
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| 6. |
- Tatara, MR, et al.
(författare)
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Effects of alpha-ketoglutarate on bone homeostasis and plasma amino acids in turkeys
- 2005
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Ingår i: Poultry Science. - 1525-3171. ; 84:10, s. 1604-1609
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Tidskriftsartikel (refereegranskat)abstract
- The objective of the study was to evaluate the effect of denervation and alpha-ketoglutarate (AKG) administration on the development of osteopenia in the turkey radius. At 22 d of age, all turkeys were subjected to neurectomy of the right radius. Control turkeys were given a saline solution into the crop each day for 97 d. Experimental turkeys were given 0.4 g of AKG/kg of BW into the crop each day. After 98 d, BW was not affected by the AKG treatment. Volumetric bone mineral density of the radius was measured by quantitative computed tomography. Mechanical properties were tested using a 3-point bending test. Cross-sectional area, second moment of inertia, and mean relative wall thickness were measured as well. An-Lino acid concentrations were assessed with the use of ion-exchange chromatography. Denervation had a negative effect on all bone characteristics that were measured except bone length. The AKG had a positive effect on all bone characteristics except bone length. Plasma concentrations of proline and leucine were increased by AKG, whereas concentrations of taurine and glutamine were decreased. The turkey radius appears to be a good model for studying osteopenia because its development can be affected by treatments such as denervation and AKG administration.
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| 7. |
- Tatara, MR, et al.
(författare)
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Influence of alpha-ketoglutarate on cortical bone density, geometrical properties and mechanical endurance of the humerus in turkeys
- 2004
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Ingår i: Bulletin of the Veterinary Institute in Puławy. - 0042-4870. ; 48:4, s. 461-465
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to estimate an influence of alpha-ketoglutarate (AKG) on volumetric bone density and geometrical and mechanical properties of the humerus in farm turkeys. All birds were kept under standard rearing conditions with constant access to water and appropriate feed, supplied in accordance with the stage of production cycle. Bone samples were collected 14 weeks after beginning of the experiment. To the first group belonged the control turkeys. The second group of animals consisted of turkeys that were treated with AKG from the 22(nd) to 110(th) d of life. Using Quantitative Computed Tomography (QCT) method, volumetric bone density of the cortical bone in the humerus was estimated. Both the mechanical and geometrical properties of the bone were assessed according to Ferretti method. The obtained results showed anabolic influence of enteral AKG administration on cortical bone density, geometrical properties and mechanical endurance of the humerus. Moreover, the humerus in turkey may be utilised as a model of cortical bone for further investigations of factors conditioning skeletal homeostasis.
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