| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Wang, Yaling, et al.
(författare)
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Enhanced performance and stability of inverted planar perovskite solar cells by incorporating 1,6-diaminohexane dihydrochloride additive
- 2018
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Ingår i: Solar Energy Materials and Solar Cells. - : ELSEVIER SCIENCE BV. - 0927-0248 .- 1879-3398. ; 188, s. 140-148
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Tidskriftsartikel (refereegranskat)abstract
- Herein, 1,6-Diaminohexane Dihydrochloride (1,6-DD) is introduced into perovskite precursors to fabricate the inverted planar perovskite solar cells. By regulating the concentration of 1,6-DD additive, the average power conversion efficiency (PCE) of perovskite solar cells is enhanced by 20%. The champion device achieves a relatively high PCE of 17% and an excellent fill factor of 80.1%. The PCE of the large-area (1 cm(2)) device also reaches to 13.68%. After exposure to the air for 16 days, the device with 1,6-DD additive still retains above 90% of the initial efficiency, exhibiting good stability. We demonstrate that a small amount of 1,6-DD affects the crystallization dynamic, yielding ideal perovskite film with enhanced crystallinity and enlarged grain size. The two terminal -NH3+ groups passivates the vacancy defects at the perovskite crystal surface, suppressing charge recombination and facilitating charge transportation effectively. Meanwhile, adjacent crystal surfaces are linked through the hexane alkyl chain of 1,6-DD molecule, which enhances the interaction between perovskite grains and anchors the microstructure of perovskite to some degree. Hydrophobic hexane alkyl chains also increase the moisture resistance of perovskite film. Thus, an easy and effective way is provided for fabricating efficient and stable perovskite solar cells.
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| 3. |
- Xie, Yuan, et al.
(författare)
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Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma
- 2023
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Ingår i: Neuro-Oncology. - : Oxford University Press. - 1522-8517 .- 1523-5866. ; 25:6, s. 1073-1084
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Tidskriftsartikel (refereegranskat)abstract
- Background: Systemic delivery of anti-tumor therapeutic agents to brain tumors is thwarted by the blood-brain barrier (BBB), an organotypic specialization of brain endothelial cells (ECs). A failure of pharmacological compounds to cross BBB is one culprit for the dismal prognosis of glioblastoma (GBM) patients. Identification of novel vascular targets to overcome the challenges posed by the BBB in tumors for GBM treatment is urgently needed.Methods: Temozolomide (TMZ) delivery was investigated in CT2A and PDGFB-driven RCAS/tv-a orthotopic glioma models. Transcriptome analysis was performed on ECs from murine gliomas. Mfsd2a deficient, Cav1 deficient, and Mfsd2a EC-specific inducible mice were developed to study the underlying molecular mechanisms.Results: We demonstrated that inhibiting Wnt signaling by LGK974 could increase TMZ delivery and sensitize glioma to chemotherapy in both murine glioma models. Transcriptome analysis of ECs from murine gliomas revealed that Wnt signaling inhibition enhanced vascular transcytosis as indicated by the upregulation of PLVAP and downregulation of MFSD2A. Mfsd2a deficiency in mice enhances TMZ delivery in tumors, whereas constitutive expression of Mfsd2a in ECs suppresses the enhanced TMZ delivery induced by Wnt pathway inhibition in murine glioma. In addition, Wnt signaling inhibition enhanced caveolin-1 (Cav1)-positive caveolae-mediated transcytosis in tumor ECs. Moreover, Wnt signaling inhibitor or Mfsd2a deficiency fails to enhance TMZ penetration in tumors from Cav1-deficient mice.Conclusions: These results demonstrated that Wnt signaling regulates MFSD2A-dependent TMZ delivery through a caveolae-mediated EC transcytosis pathway. Our findings identify Wnt signaling as a promising therapeutic target to improve drug delivery for GBM treatment.
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| 4. |
- Wang, Yaling, et al.
(författare)
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Enhanced performance and stability in polymer photovoltaic cells using lithium benzoate as cathode interfacial layer
- 2011
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Ingår i: SOLAR ENERGY MATERIALS AND SOLAR CELLS. - : Elsevier Science B.V., Amsterdam.. - 0927-0248. ; 95:4, s. 1243-1247
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Tidskriftsartikel (refereegranskat)abstract
- We report the enhanced performance and stability of polymer solar cells based on regioregular poly(3-hexylthiophene)(P3HT) and methanofullerene [6,6]-phenyl C-61-butyric acid methyl ester (PCBM) blend using lithium benzoate (C6H5COOLi) as cathode buffer layer between the active layer and the Al cathode. The effects of the C6H5COOLi thickness on the performance of polymer solar cell are also investigated. Under 100 mW/cm(2) white light illumination, the device with 1 nm thick C6H5COOLi as cathode buffer layer exhibits power conversion efficiency (PCE) as high as 3.41 +/- 0.07% and the device stability is greatly extended. Compared to the solar cell with LiF/AI cathode, the PCE is increased ca. 9.4%. Introduction of C6H5COOLi buffer layer effectively increases the shunt resistance and improves the photo-generated charge collection. The improved performance may attribute to the dissociation of semi-conducting C6H5COOLi upon deposition to liberate Li with a low work function, which reduces the interface resistance of the active layer and the cathode and enhances the interior electric field that may result in efficient charge transportion. In addition, the C6H5COOLi layer may serve as an effective oxygen and moisture diffusion barrier for the organic solar cells. Therefore. C6H5COOLi is a promising candidate as an interlayer to improve the efficiency of electron collection and to reduce the ambience influence on the stability of polymer solar cells.
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