| 2. |
- Gross, Sean M., et al.
(författare)
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A multi-omic analysis of MCF10A cells provides a resource for integrative assessment of ligand-mediated molecular and phenotypic responses
- 2022
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Ingår i: Communications Biology. - : Springer Nature. - 2399-3642. ; 5:1
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Tidskriftsartikel (refereegranskat)abstract
- The phenotype of a cell and its underlying molecular state is strongly influenced by extracellular signals, including growth factors, hormones, and extracellular matrix proteins. While these signals are normally tightly controlled, their dysregulation leads to phenotypic and molecular states associated with diverse diseases. To develop a detailed understanding of the linkage between molecular and phenotypic changes, we generated a comprehensive dataset that catalogs the transcriptional, proteomic, epigenomic and phenotypic responses of MCF10A mammary epithelial cells after exposure to the ligands EGF, HGF, OSM, IFNG, TGFB and BMP2. Systematic assessment of the molecular and cellular phenotypes induced by these ligands comprise the LINCS Microenvironment (ME) perturbation dataset, which has been curated and made publicly available for community-wide analysis and development of novel computational methods ( synapse.org/LINCS_MCF10A ). In illustrative analyses, we demonstrate how this dataset can be used to discover functionally related molecular features linked to specific cellular phenotypes. Beyond these analyses, this dataset will serve as a resource for the broader scientific community to mine for biological insights, to compare signals carried across distinct molecular modalities, and to develop new computational methods for integrative data analysis.
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| 3. |
- Mereu, R.F., et al.
(författare)
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The Seismicity of the Western Lake Ontario Area : Results from the Southern Ontario Seismic Network (SOSN), 1992-2001
- 2002
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Ingår i: Seismological Research Letters. - : Seismological Society of America (SSA). - 0895-0695 .- 1938-2057. ; 73:4, s. 534-551
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Tidskriftsartikel (refereegranskat)abstract
- The Southern Ontario Seismic Network (SOSN) consists of eleven three-component short-period seismic stations, located mainly in the Toronto-Hamilton-Niagara area of Ontario, Canada. The network has been in operation by the University of Western Ontario (UWO) for Ontario Power Generation (OPG) since 1991 with the purpose of obtaining information on the seismicity and seismic hazards of a region of southern Ontario in which a number of nuclear power stations are located. Over the past decade, an average of more than ten local earthquakes per year in the western Lake Ontario area was detected by the SOSN. Most of the events were in the 2–3 magnitude (MN) range. The largest events during this time took place in the surrounding regions—Pymatuning, northwestern Pennsylvania (285 km southwest from Toronto, just south of Lake Erie, 25 September 1998, MN 5.4), northern Ontario/Quebec border (325 km north of Toronto, 1 January 2000, MN 5.2), Ashtabula, Ohio (262 km southwest of Toronto, 26 January 2001, MN 4.4), and Au Sable Forks, New York (436 km east of Toronto, 20 April 2002, MN 5.1). The largest earthquake (MN 3.8) in the western Lake Ontario region during the past ten years occurred on 26 November 1999 in Lake Ontario, 16 km southeast of the town of Pickering, which lies just east of Toronto. The estimated location uncertainty (±2 km) is significantly better than that which was possible before 1991. The focal depths, though poorly constrained for most events, are shown to lie in the 3–15 km range, well within the Grenvillian rocks of the Precambrian Shield. The new seismicity map shows that a definite pattern is emerging in the SOSN data set in Lake Ontario, one which is significantly different from the past historical earthquake patterns obtained when the instrumental coverage was poor. Most events occur in scattered clusters in the western part of Lake Ontario and the northwestern corner of New York State. The area of seismicity does not extend significantly to the north of western Lake Ontario and appears to end to the west rather abruptly along a 30 km small fault line running from south of Hamilton in a north-northeasterly direction to Burlington, Ontario. Although the area of seismicity coincides with a region of linear magnetic anomaly trends (suggesting a strong structural fabric in the basement rocks), the correlation of seismicity of the new SOSN data set with magnetic lineaments is still unclear. The cause of the seismicity is speculated to be related to water flows along various fissures below the lake. It is known from induced seismicity studies of reservoirs that the presence of fluids can cause earthquakes by changing the pore pressure and reducing the friction along any faults which may be present. From seismic reflection studies, dipping structures and shear zones have been imaged to extend southeastward under Lake Ontario. This may explain why most of the earthquakes are occurring under the lake or southeast of the lake.
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