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1.	Ades, M., et al. (author) Global Climate : in State of the climate in 2019 2020 In: Bulletin of The American Meteorological Society - (BAMS). - : American Meteorological Society. - 0003-0007 .- 1520-0477. ; 101:8, s. S17-S127 Journal article (peer-reviewed)
2.	Ades, M., et al. (author) GLOBAL CLIMATE 2020 In: BULLETIN OF THE AMERICAN METEOROLOGICAL SOCIETY. - 0003-0007 .- 1520-0477. ; 101:8 Journal article (peer-reviewed)
3.	Xiao, Zhiqiang, et al. (author) Systematic Engineering of Saccharomyces cerevisiae Chassis for Efficient Flavonoid-7-O-Disaccharide Biosynthesis 2023 In: ACS Synthetic Biology. - 2161-5063. ; 12:9, s. 2740-2749 Journal article (peer-reviewed)abstract Flavonoids are an essential class of secondary metabolites found in plants and possess various nutritional, medicinal, and agricultural properties. However, the poor water solubility of flavonoid aglycones limits their potential applications. To overcome this issue, glycosylation is a promising approach for improving water solubility and bioavailability. In this study, we constructed a flavonoid-7-O-disaccharide biosynthetic pathway with flavonoid aglycones as substrates in Saccharomyces cerevisiae. Subsequently, through metabolic engineering and promoter strategies, we constructed a UDP-rhamnose regeneration system and optimized the UDP-glucose (UDPG) synthetic pathway. The optimized strain produced up to 131.3 mg/L eriocitrin. After this, the chassis cells were applied to other flavonoids, with substrates such as (2S)-naringenin, (2S)-hesperetin, diosmetin, and (2S)-eriodictyol, which resulted in the synthesis of 179.9 mg/L naringin, 276.6 mg/L hesperidin, 249.0 mg/L neohesperidin, 30.4 mg/L diosmin, and 100.7 mg/L neoeriocitrin. To the best of our knowledge, this is the first report on the biosynthesis of flavonoid-7-O-disaccharide.

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Type of publication: journal article (3)

Type of content: peer-reviewed (3)

Author/Editor: Rozanov, A. (2); Scanlon, T. (2); Sharma, S. (2); Allan, Rob (2); Becker, Andreas (2); Benedetti, Angela (2); show more...; Berry, David I. (2); Bosilovich, Michael ... (2); Boucher, Olivier (2); Christiansen, Hanne ... (2); Christy, John R. (2); Chung, E. S. (2); Coldewey-Egbers, Mel ... (2); Cooper, Owen R. (2); Davis, Sean M. (2); De Eyto, Elvira (2); De Jeu, Richard A.M. (2); Degasperi, Curtis L. (2); Degenstein, Doug (2); Di Girolamo, Larry (2); Dokulil, Martin T. (2); Donat, Markus G. (2); Dorigo, Wouter A. (2); Phillips, C. (2); Anderson, J (2); Gobron, N. (2); Kaiser, J. W. (2); Kratz, D. P. (2); Sawaengphokhai, P. (2); Willett, K. M. (2); Kelly, S. (2); Ades, M. (2); Adler, R. (2); Allan, R. P. (2); Arguez, Anthony (2); Arosio, C. (2); Augustine, J. A. (2); Barichivich, J. (2); Barnes, J. (2); Beck, H. E. (2); Bellouin, Nicolas (2); Blenkinsop, Stephen (2); Bock, Olivier (2); Buehler, S. A. (2); Carrea, Laura (2); Chouza, F. (2); Compo, Gil P. (2); Covey, Curt (2); Crotwell, A. (2); VanderSat, B. V. (2); show less...

University: University of Gothenburg (1); Uppsala University (1); Chalmers University of Technology (1)

Language: English (3)

Research subject (UKÄ/SCB): Natural sciences (2); Agricultural Sciences (1)

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