| 1. |
|
|
| 2. |
- Ghasemi, J., et al.
(författare)
-
Spectroscopic characterization of thiazole orange-3 DNA interaction
- 2008
-
Ingår i: Applied Biochemistry and Biotechnology. - : Springer Science and Business Media LLC. - 0273-2289 .- 1559-0291. ; 149:1, s. 9-22
-
Tidskriftsartikel (refereegranskat)abstract
- The interaction of a new derivative of thiazole orange (TO-3) with calf thymus DNA (ctDNA) has been investigated by fluorescence and absorption spectroscopy. When TO-3 binds to ctDNA, absorption bands exhibit significant hypochromicity at low base pair/dye ratio (BP/D ratio), and high BP/D show hyperchromicity with red shift. The spectral changes are attributed to the different species formed between TO-3 and ctDNA in the titration course of the dye molecule with DNA. Multivariate curve resolutions-alternating least squares (MCR-ALS) is applied to the absorption measurements recorded to recover the concentration profiles and the pure spectra of the DNA/TO-3 complexes involved in the process. The binding constant and size of the binding site have been determined spectrophotometrically using the McGhee von Hippel equation. MCR-ALS has been used to reveal the precise concentration profiles of all detectable species formed between ctDNA and TO-3 and their pure spectral profiles. © 2008 Humana Press Inc.
|
|
| 3. |
|
|
| 4. |
- Ma, Li-Jun, et al.
(författare)
-
Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a whole-genome duplication.
- 2009
-
Ingår i: PLoS genetics. - : Public Library of Science (PLoS). - 1553-7404. ; 5:7, s. e1000549-
-
Tidskriftsartikel (refereegranskat)abstract
- Rhizopus oryzae is the primary cause of mucormycosis, an emerging, life-threatening infection characterized by rapid angioinvasive growth with an overall mortality rate that exceeds 50%. As a representative of the paraphyletic basal group of the fungal kingdom called "zygomycetes," R. oryzae is also used as a model to study fungal evolution. Here we report the genome sequence of R. oryzae strain 99-880, isolated from a fatal case of mucormycosis. The highly repetitive 45.3 Mb genome assembly contains abundant transposable elements (TEs), comprising approximately 20% of the genome. We predicted 13,895 protein-coding genes not overlapping TEs, many of which are paralogous gene pairs. The order and genomic arrangement of the duplicated gene pairs and their common phylogenetic origin provide evidence for an ancestral whole-genome duplication (WGD) event. The WGD resulted in the duplication of nearly all subunits of the protein complexes associated with respiratory electron transport chains, the V-ATPase, and the ubiquitin-proteasome systems. The WGD, together with recent gene duplications, resulted in the expansion of multiple gene families related to cell growth and signal transduction, as well as secreted aspartic protease and subtilase protein families, which are known fungal virulence factors. The duplication of the ergosterol biosynthetic pathway, especially the major azole target, lanosterol 14alpha-demethylase (ERG11), could contribute to the variable responses of R. oryzae to different azole drugs, including voriconazole and posaconazole. Expanded families of cell-wall synthesis enzymes, essential for fungal cell integrity but absent in mammalian hosts, reveal potential targets for novel and R. oryzae-specific diagnostic and therapeutic treatments.
|
|
| 5. |
- van den Dool, Annemieke, et al.
(författare)
-
The future is distributed: a vision of sustainable economies
- 2009
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- “The Future is distributed: a vision of sustainable economies” is a collection of case studies on distributed economies, a concept describing sustainable alternatives to the existing business models. The authors of this publication are international Masters students of the Environmental Sciences, Policy and Management Programme at the International Institute for Industrial Environmental Economics at Lund University in Sweden. The aim of their work is to demonstrate that local, small-scale, community-based economies are not just part of the theory, but have already been implemented in various sectors and geographical settings.
|
|
